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Method for preparing 1, 3-propanediol by microbial fermentation method

A microbial fermentation method and propylene glycol technology, applied in the field of 1,3-propanediol preparation, can solve the problems of low 1,3-propanediol content and low utilization rate of glycerin

Inactive Publication Date: 2020-07-07
尹玉琴
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the above process, in addition to 1,3-propanediol, there will be acetic acid, ethanol, 2,3-butanediol, lactic acid and other by-products in the fermentation broth, resulting in low utilization rate of glycerin and 1,3-propanediol content in the fermentation product. Low

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  • Method for preparing 1, 3-propanediol by microbial fermentation method

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Experimental program
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Embodiment 1~3

[0020] (1) Strains: Klebsiella pneumoniae ( Klebsiella pneumoniae )

[0021] (2) Medium:

[0022] Seed activation medium: LB medium, 10g / L peptone, 5.0g / L yeast powder, 10g / L NaCl, pH 7.0.

[0023] Seed medium: 5g / L yeast powder, 10g / L glucose, 5g / L peptone, 5g / L NaCl, 6g / L K 2 HPO 4 ·3H 2 O, 3g / L of KH 2 PO 4 , 2g / L NH 4 Cl, 1.5g / L Na 2 SO 4 , 0.5g / L of MgSO 4 ·7H 2 O;

[0024] Fermentation medium: 5g / L yeast powder, 6g / L K 2 HPO 4 ·3H 2 O, 3g / L of KH 2 PO 4 , 3g / L NH 4 Cl, 1.5g / L Na 2 SO 4 , 1.5g / L of MgSO 4 ·7H 2 O, 40mg / L FeCl 3 ·6H 2 O, 75mg / L of CoCl 2 ·6H 2 O, vitamin B12 of 60mg / L, NiCl of 40mg / L 2 And 40g / L of glycerin.

[0025] (3) Fermentation process

[0026] Klebsiella pneumoniae was inserted into the seed activation medium for activation, and cultured at 37°C for 24 hours;

[0027] Inoculate the activated strains into the seed medium, and cultivate them at 37°C and 100rpm for 12h, so that the concentration of the strains reaches OD 6...

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Abstract

The invention provides a method for preparing 1, 3-propanediol by a microbial fermentation method. The method comprises the following steps of taking glycerol as a substrate, performing anaerobic or micro-aerobic fermentation on microorganism in a fermentation culture medium to obtain 1, 3-propanediol; and in the fermentation medium and / or in a fermentation process, adding calcium lactobionate andvitamin C, wherein the mass ratio of calcium lactobionate to vitamin C is 1 to (0.1-0.5). Experimental results show that the glycerol is used as the substrate for fermentation to prepare 1, 3-propanediol, and calcium lactobionate and vitamin C are added in the fermentation process, so that the yield of 1, 3-propanediol can be greatly increased, and the production of a by-product 2, 3-butanediol is reduced.

Description

technical field [0001] The invention relates to the technical field of 1,3-propanediol preparation, in particular to a method for preparing 1,3-propanediol by a microbial culture and fermentation method. Background technique [0002] 1,3-propanediol (1,3-propanediol, 1,3-PDO), the molecular formula is HOCH 2 -CH 2 -CH 2 OH is the basic raw material of many polymers, which can be used in cosmetics, liquid detergents, antifreeze, clothing, interior decoration materials, engineering polymers and many other fields. [0003] The synthesis methods of 1,3-propanediol are divided into two categories: chemical method and biological fermentation method. Among them, chemical methods mainly include acrolein hydration hydrogenation method and ethylene oxide carbonylation method. Compared with the chemical method, the biological fermentation method has the characteristics of mild conditions, cheap and easy-to-obtain raw materials, and low cost, and has become a 1,3-propanediol product...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12N1/20C12R1/22C12R1/01C12R1/145
CPCC12N1/20C12P7/18
Inventor 尹玉琴
Owner 尹玉琴