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Doxorubicin nanoparticle and its preparation method and application, sonodynamic combined chemotherapy drug for tumor treatment

A nanoparticle, doxorubicin technology, applied in antitumor drugs, drug combinations, drug delivery, etc., can solve the problems of multi-resistant doxorubicin, high cost and difficulty in clinical application, and achieve overcoming drug resistance. , the effect of enhancing efficacy and attenuating tumor treatment, reducing preparation cost

Active Publication Date: 2022-06-21
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] One of the purposes of the present invention is to provide a doxorubicin nanoparticle to solve the problem of multi-drug resistance to doxorubicin in the existing combined gene therapy and oxygen supply therapy, and the technical problems of high cost and difficulty in clinical application

Method used

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  • Doxorubicin nanoparticle and its preparation method and application, sonodynamic combined chemotherapy drug for tumor treatment
  • Doxorubicin nanoparticle and its preparation method and application, sonodynamic combined chemotherapy drug for tumor treatment
  • Doxorubicin nanoparticle and its preparation method and application, sonodynamic combined chemotherapy drug for tumor treatment

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preparation example Construction

[0047] According to a second aspect of the present invention, the present invention provides a method for preparing doxorubicin nanoparticles, comprising the steps of: mixing doxorubicin and a nanoparticle carrier uniformly, so that the doxorubicin is loaded on the nanoparticle carrier , to obtain doxorubicin nanoparticles.

[0048] In the present invention, doxorubicin is loaded on the nanoparticle carrier through chemical bonds or encapsulation, wherein the chemical bonds include covalent bonds, ionic bonds and metal bonds.

[0049] The preparation method of the doxorubicin nanoparticle provided by the invention is simple in process and low in raw materials, and can significantly reduce the preparation cost of the doxorubicin nanoparticle.

[0050] In a preferred embodiment of the present invention, the nanoparticle carrier is a polyamino acid nanoparticle. Selecting polyamino acid nanoparticles as the doxorubicin carrier has the following beneficial effects:

[0051] (1) ...

Embodiment 1

[0084] This embodiment provides a kind of doxorubicin nanoparticle, which is prepared according to the following steps:

[0085] (1) PBS solution of sodium polyglutamate (PGA), aqueous solution of EDC, aqueous solution of NHS, PBS solution of cystamine dihydrochloride (Cys) and PBS solution of doxorubicin were prepared respectively, wherein the PBS solution of PGA The concentration of the solution is 1 mg / mL, and the pH value is 5.5; the concentration of EDC in water is 5.2 mg / mL, the concentration of NHS in water is 3.2 mg / mL, the concentration of Cys in PBS is 1 mg / mL, and the concentration of DOX in PBS is 1 mg / mL. The concentration is 0.5mg / mL, and the pH value is 7.4;

[0086] (2) Take 1 mL of the PBS solution of PGA, and quickly add 0.5 mL of EDC aqueous solution and 0.5 mL of NHS aqueous solution dropwise to the solution and stir at room temperature for 15 min;

[0087](3) adding 1 mL DOX in PBS solution and 1 mL Cys in PBS solution dropwise to the solution obtained in...

Embodiment 2

[0090] This example provides a doxorubicin nanoparticle, which is different from Example 1 in that in step (1), the concentration of the prepared PGA in PBS solution is 0.5 mg / mL, and the remaining steps are the same as the implementation of Example 1 is the same and will not be repeated here.

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Abstract

The invention provides a doxorubicin nanoparticle, its preparation method and application, and a drug for treating tumors with sonodynamics combined with chemotherapy, and relates to the technical field of nanomedicine. The doxorubicin nanoparticle includes adriamycin and a nanoparticle carrier. The doxorubicin is loaded on the nanoparticle carrier, which alleviates the technical problems of high cost and difficulty in clinical application to solve the multidrug resistance of doxorubicin by combined gene therapy and oxygen supply therapy. The doxorubicin nanoparticle provided by the present invention can not only improve the biocompatibility and cell phagocytosis efficiency of doxorubicin by loading doxorubicin on the nanoparticle carrier, but also can generate reactive oxygen species under the action of ultrasound, inhibit The efflux of doxorubicin by tumor cells overcomes the drug resistance of tumor cells and realizes anti-tumor therapy with synergistic and attenuated toxicity. It has broad application prospects in the fields of biomedicine, disease diagnosis and sonodynamic combined chemotherapy .

Description

technical field [0001] The invention relates to the field of nano-drugs, in particular to a doxorubicin nano-particle, a preparation method and application thereof, and a drug for treating tumors in combination with sound dynamics and chemotherapy. Background technique [0002] Doxorubicin is a broad-spectrum antitumor drug that can inhibit the synthesis of RNA and DNA, and has a killing effect on various tumors and tumor cells in various growth cycles. However, doxorubicin has strong toxic and side effects on normal cells, and is prone to multidrug resistance to tumor cells, which limits the application of doxorubicin in tumor therapy. [0003] The rapid development of nanotechnology provides new ideas for solving the problems faced in the process of doxorubicin chemotherapy. The nano-drug loading system can improve the bioavailability and biocompatibility of doxorubicin, and regulate the circulation and distribution in the body. At present, the combination of gene therap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K31/704A61K41/00A61K47/69A61P35/00
CPCA61K9/5146A61K47/6935A61K31/704A61P35/00A61K41/0033A61K2300/00
Inventor 蔡林涛梁锐晶张升平陈华清马爱青尹婷
Owner SHENZHEN INST OF ADVANCED TECH
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