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Method for determining content of sex pheromone in tobacco beetle sex pheromone lure

A technology for tobacco beetles and pheromones, applied in the field of detection and analysis, can solve the problems of inability to accurately measure the content of sex pheromones in lure tobacco beetles, complex and diverse components, etc., and achieve the effects of high accuracy, good repeatability, and high sensitivity

Active Publication Date: 2020-07-10
CHINA TOBACCO FUJIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content and stability of tobacco beetle sex pheromones in tobacco beetle pheromone lures have an important impact on the effectiveness of traps, so it is necessary to accurately know the content of tobacco beetle sex pheromones in tobacco beetle sex pheromone lures, However, the components in tobacco beetle sex pheromone lures are complex and diverse, and general detection methods (such as GC-MS) cannot accurately determine the content of tobacco beetle sex pheromones in lures

Method used

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  • Method for determining content of sex pheromone in tobacco beetle sex pheromone lure
  • Method for determining content of sex pheromone in tobacco beetle sex pheromone lure
  • Method for determining content of sex pheromone in tobacco beetle sex pheromone lure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of a series of standard solutions:

[0045] Add accurately weighed (7S)-(one)-4,6-dimethyl-7-hydroxyl-3-nonanone standard 25 mg (accurate to 0.1 mg) into a 10 mL volumetric flask, add 0.1% (mass) concentration) sodium borohydride in ethanol to obtain (7S)-(one)-4,6-dimethyl-7-hydroxyl-3-nonanone with a concentration of 2500 μg / mL grade 1 standard solution. Pipette 5mL, 2mL, 1mL, and 0.2mL of grade 1 standard solutions into 10mL volumetric flasks, and dilute to volume with ethanol containing 0.1% (mass concentration) sodium borohydride to prepare grade 2-5 standard solutions.

[0046] (2) Pre-processing:

[0047] Put a newly opened tobacco beetle pheromone lure 1 into a 20mL headspace bottle, add 1ml of ethanol containing 0.1% (mass concentration) sodium borohydride to submerge the lure to obtain a sample, and seal it for later use.

[0048] (3) Detection:

[0049] The series of standard solutions and the samples obtained in step (2) were sent to the g...

Embodiment 2

[0060] Measure the content of (7S)-(one)-4,6-dimethyl-7-hydroxyl-3-nonanone in the tobacco beetle sex pheromone lure 2-13 according to the method in embodiment 1, wherein, lure Cores 2-6 are newly opened, and the rest are lures placed for a period of time after unsealing. The results are shown in Table 1.

[0061] Table 1 Measurement results

[0062] Content of (7S)-(one)-4,6-dimethyl-7-hydroxy-3-nonanone (μg / piece) Lure 2 550.50 lure 3 554.64 lure 4 515.39 Lure 5 635.96 Lure 6 302.46 Lure 7 54.48 Lure 8 39.43 Lure 9 36.62 lure 10 36.29 Lure 11 36.19 lure 12 36.08 Lure 13 36.45

[0063] It can be known from Table 1 that the content of sex pheromone in the newly opened lure is relatively high; after opening for a period of time, the content of sex pheromone in the lure decreases significantly, which seriously affects the effectiveness of the lure. Therefore, it is necessary to measure and m...

Embodiment 3

[0064] Repeatability, rate of recovery, limit of detection and limit of quantification of embodiment 3 method

[0065] Take the fifth-grade standard solution in Example 1 and repeat the sample injection test ten times according to the method in Example 1, and use three times and ten times of the deviation as the detection limit and quantification limit respectively, as shown in Table 2.

[0066] Add 500 μ g (7S)-(one)-4,6-dimethyl-7-hydroxyl-3-nonanone standard substance on the lure 1 basis of embodiment 1, obtain the spiked sample, then add the spiked sample according to Steps (2)-(4) in Example 1 were pre-treated, detected and data processed, the recovery rate was calculated, and the experiment was repeated six times in parallel. The results are shown in Table 2.

[0067] Repeatability, rate of recovery, limit of detection and limit of quantitation results of the method of the present invention in table 2

[0068]

[0069] As can be seen from Table 2, the relative stan...

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Abstract

The invention belongs to the field of detection and analysis, and particularly relates to a method for determining content of sex pheromone in a tobacco beetle sex pheromone lure. The method comprisesthe following steps: (1) immersing the tobacco beetle sex pheromone lure with a solvent to obtain a mixture; wherein the solvent is ethanol containing sodium borohydride, and the mass of sodium borohydride accounts for 0.05%-1% of the mass of the solvent; and (2) introducing the mixture into a gas chromatograph-triple quadrupole mass spectrometer through a headspace sample injector with a trap, and then carrying out detection to obtain a detection result. The invention also discloses specific operating conditions of the headspace sample injector with the trap. The method for determining the content of the tobacco beetle sex pheromone in the tobacco beetle sex pheromone lure and the tobacco beetle sex pheromone trap is high in repeatability, high in sensitivity, low in detection limit andhigh in accuracy.

Description

technical field [0001] The invention belongs to the field of detection and analysis, and in particular relates to a method for measuring the content of sex pheromone in tobacco beetle sex pheromone lures. Background technique [0002] Tobacco beetle is one of the main pests in tobacco storage warehouses in my country. Tobacco beetles have a wide distribution, a large number of reproduction, and serious damage. They not only eat tobacco leaves to form holes, which affect the tobacco silk production rate, but also excrete a large amount of feces and leave insect corpses, resulting in the deterioration of tobacco leaf flavor and quality. Tobacco beetles have caused serious losses to the tobacco industry. According to rough estimates, the direct economic losses caused by tobacco beetles in the country amount to more than 1 billion yuan every year. [0003] At present, tobacco beetle control mainly uses tobacco beetle pheromone traps to monitor the distribution and density of to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 黄惠贞张建平刘秀彩谢卫李巧灵张鼎方刘泽春梁晖林艳黄华发周培琛黄朝章许寒春
Owner CHINA TOBACCO FUJIAN IND
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