Highland barley beta-glucan and purification process thereof

Abstract

The invention discloses highland barley beta-glucan and a purification process thereof. The purification process comprises the steps of macroporous resin decoloration, agarose gel column chromatography molecular sieve purification, drying and the like. According to the invention, a new highland barley beta-glucan is separated from highland barley; the beta-glucan is subjected to structural identification by adopting a plurality of methods taking high performance gel chromatography-differential-laser scattering detection (HPSEC-dRI-LS) and a nuclear magnetic resonance carbon spectrum (13C-NMR)as main methods; through an enzyme inhibition experiment, the hypoglycemic activity of the highland barley beta-glucan which is purified by the method and has a definite structure and sequence is verified, and a foundation is laid for developing functional foods for regulating blood sugar.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a highland barley beta-glucan and a purification process thereof. Background technique [0002] Highland barley, barley, is one of the staple foods of people in the Qinghai-Tibet Plateau, as well as livestock feed and industrial raw material crops. According to testing, the content of β-glucan in highland barley is relatively high, reaching 4%-8%. The β-glucan is a linear polysaccharide in which glucopyranose is linked by β-(1→3) or β-(1→4) glycosidic bonds. [0003] In recent years, highland barley β-glucan has received great attention in the field of food science, and many important physiological functions and physiological activities of β-glucan have been gradually recognized. It has been reported that highland barley β-glucan can be used as a regulator of innate immunity and acquired immunity, with good hypoglycemic and hypolipidemic functions, and also shows good application pro...

AI Technical Summary

Problems solved by technology

Due to the limited research on the structure and activity of highland barley β-glucan, its application is limited. Most of them only grind highland barley to make pasta, noodle cakes and other common foods

Although highland barley β-glucan extracts have been found to have hypoglycemic activity, the mechanism of hypoglycemic effect has not been clarified due to the difficulty in purification of polysaccharides with uniform molecular weight and complex structure identification, which hinders their development as functional foods for preventing and treating diabetes and even new drug process

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