Double-block DNA modified up-conversion nanoparticle and preparation method and application thereof
A nanoparticle and diblock technology, applied in the field of nanomaterials, can solve the problems of cumbersome modification process, limit the wide application, weaken the luminescence of nanoparticles, etc., and achieve simple modification process, broad application prospects, good stability and monodispersity Effect
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[0032] The present invention also provides the preparation method of the double-block DNA modified up-conversion nanoparticles, comprising the following steps: 1) cleaning the up-conversion nanoparticles with hydrochloric acid to obtain surface-free up-conversion nanoparticles; The upconverting nanoparticles without ligands on the surface are mixed with the diblock DNA solution to obtain a mixed solution; 3) adding 10×TB-Na + buffer solution, so that the final concentration of NaCl in the system is 0.08-0.12mol / L; and then incubate to obtain double-block DNA modified up-conversion nanoparticles.
[0033] In the present invention, upconverting nanoparticles are washed with hydrochloric acid to obtain upconverting nanoparticles without ligands on the surface. In the present invention, the concentration of the hydrochloric acid is preferably 0.08-0.12 mol / L, more preferably 0.1 mol / L; the number of cleanings is preferably 1-3 times, more preferably 2 times. In the present inventio...
Embodiment 1
[0041] Surface hexagonal NaYF 4 18% Yb, 2% Er upconversion nanoparticles DNA modification and the assembly of DNA modified upconversion nanoparticles and gold nanoparticles (5nm, DNA-AuNPs)
[0042] Hexagonal NaYF 4 : 18%Yb, 2%Er up-conversion nanoparticles preparation: Weigh 0.8mmol anhydrous YCl 3 , 0.18 mmol anhydrous YbCl 3 and 0.02 mmol anhydrous ErCl 3 , Measure 7.5mL oleic acid (OA) and 17.5mL octadecene (ODE) into a 50mL three-necked round bottom flask together. Open the reflux condenser and quickly inject nitrogen gas, place the round-bottomed flask in a heating mantle, heat to 150°C, stir at constant temperature for 30 min, and then cool to room temperature. Take off the reflux condenser, add 2.5mmol NaOH and 5.5mmol NH in the round bottom flask 4 F methanol solution, heated to 100°C and then stirred at constant temperature for 30min. Thereafter, the reactant was heated up to 300° C., reacted at constant temperature for 1 h, and finally the reactant was cooled ...
Embodiment 2
[0061] Hexagonal NaYF 4 : Surface DNA modification of 20% Yb, 5% Tm upconversion nanoparticles and assembly of DNA modified upconversion nanoparticles with gold nanoparticles (5nm, DNA-AuNPs)
[0062] Synthesis of hexagonal NaYF 4 : 20% Yb, 5% Tm up-conversion nanoparticles: Weigh 0.75mmol anhydrous YCl 3 , 0.2 mmol anhydrous YbCl 3 and 0.05 mmol anhydrous TmCl 3 , Measure 7.5mL oleic acid (OA) and 17.5mL octadecene (ODE) into a 50mL three-necked round bottom flask together. Open the reflux condenser and quickly inject nitrogen gas, place the round bottom flask in a heating mantle, heat to 150°C, stir at constant temperature for 30 min, and then cool to room temperature. After removing the reflux condenser, add 2.5mmol NaOH and 5.5mmol NH 4 The methanol solution of F was heated to 100°C and then stirred at constant temperature for 30 minutes. Thereafter, the reactant was heated up to 300° C., reacted at constant temperature for 1.5 h, and finally the reactant was cooled ...
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