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Preparation method and application of caulerpa lentillifera extract

A sea grape and extract technology is applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. , the effect of simple extraction and separation operation

Pending Publication Date: 2020-07-17
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sea grapes are extracted with organic solvents. Adding them to cosmetic formulas may cause allergies. Even if only a small amount of organic solvents remains, the extracts will cause harm to the skin and even the human body

Method used

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  • Preparation method and application of caulerpa lentillifera extract
  • Preparation method and application of caulerpa lentillifera extract
  • Preparation method and application of caulerpa lentillifera extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 sea grape extract

[0028] (a) Take by weighing 10kg of cleaned sea grapes, wash its surface with deionized water and place it in a colloid mill, after the colloid mill adds deionized water to grind it slightly, add deionized water with a mass ratio of 4 to sea grapes; add sea grapes Grape weight 0.03% sodium D-ascorbate, add sea grape weight 0.06% mixture of cellulase and pectinase, wherein the mixture of cellulase and pectinase is diluted with deionized water at a mass ratio of 1:50 ;Stir and extract for 120min at a reaction temperature of 40°C and a stirring speed of 50r / min;

[0029] (b) Heat up to 70°C and stir for 30 minutes to inactivate the enzyme; stop heating, cool down to 20°C in water circulation, use 60-mesh gauze for coarse filtration; take the filtrate, use a filter plate with a filter plate pore size of 1 μm to obtain a light green liquid;

[0030] (c) Use a filter plate with a filter plate aperture of 0.1 μm to filter, t...

Embodiment 2

[0032] Embodiment 2: the preparation of sea grape extract

[0033] (a) Take 10 kg of cleaned sea grapes, wash its surface with deionized water and place it in a colloid mill, and after the colloid mill adds deionized water to grind it slightly, add deionized water with a mass ratio of sea grapes of 20; add sea grapes Grape weight 0.03% sodium D-ascorbate, add sea grape weight 0.15% pectinase, pectinase and deionized water are diluted in a mass ratio of 1:60; at a reaction temperature of 60°C, a stirring speed of 100r / Min speed stirring extraction 60min;

[0034] (b) Heat up to 100°C and stir for 15 minutes to inactivate the enzyme; stop heating, cool down to 50°C in water circulation, and use 150-mesh gauze for coarse filtration; take the filtrate and use a filter plate with a filter plate pore size of 8 μm to obtain a light green liquid;

[0035] (c) Use a filter plate with a filter plate aperture of 1 μm to filter to obtain a translucent light green liquid; measure the pH ...

Embodiment 3

[0037] Example 3: Preparation of sea grape extract.

[0038] (a) Take 10kg of cleaned sea grapes, clean its surface with deionized water and place it in a colloid mill, after the colloid mill adds deionized water to grind it slightly, add deionized water with a mass ratio of sea grapes that is 13; add sea grapes 0.03% D-sodium ascorbate by grape weight, add 0.08% cellulase by sea grape weight, wherein cellulase and deionized water are diluted by the mass ratio of 1:70; / min speed stirring extraction 80min;

[0039] (b) Heat up to 80°C and stir for 25 minutes to inactivate the enzyme; stop heating, cool down to 40°C in water circulation, and use 100-mesh gauze for coarse filtration; take the filtrate and use a filter plate with a filter plate pore size of 5 μm to obtain a light green liquid;

[0040] (c) Use a filter plate with a filter plate pore size of 0.2 μm to obtain a translucent light green liquid; measure the pH value, solid content, and electrical conductivity, and th...

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Abstract

The invention provides a preparation method of a caulerpa lentillifera extract. The preparation method is characterized by comprising the following steps: (a) cleaning caulerpa lentilliferas with deionized water, then performing crushing, adding deionized water and an antioxidant, performing heating and stirring for extracting; (b) performing cooling, rough filtering and fine filtering; (c) performing suction filtration, and sterilizing the filtrate for antisepsis; and (d) performing precipitating with alcohol, and performing freeze-drying to obtain the caulerpa lentillifera extract. Comparedwith the prior art, the method for preparing the caulerpa lentillifera extract has the advantages of simple and convenient extraction and separation operation, high extraction rate of effective activecomponents and high purity of finally prepared products. According to the invention, water is directly used as a solvent, and under enzymolysis action of pectinase or cellulase, the extraction rate of the caulerpa lentillifera and the dissolution rate of effective components are greatly improved and the content of effective components with moisture retention and anti-aging effects is improved.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a preparation method of sea grape extract. In addition, the present invention also relates to the moisturizing and anti-aging effect of the sea grape extract extracted by the method, and specifically relates to the application of the sea grape extract in cosmetics. Background technique [0002] Anti-aging has always been the long-term focus of the cosmetics industry. Delaying skin aging and making skin more supple is one of the goals most people pursue. Environmental factors accelerate the aging of our skin to a certain extent. At the same time, with the improvement of economic level and people's higher pursuit of beauty, anti-aging cosmetics are flourishing. The basic elements of healthy skin are smooth, full and elastic. With the increase of age, the skin gradually becomes dry, wrinkled and loose, showing an aging appearance. The pathological mechanism of this aging appearance is mai...

Claims

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Application Information

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IPC IPC(8): A61K8/9722A61Q19/00A61Q19/08
CPCA61K8/9722A61Q19/00A61Q19/08A61K2800/805
Inventor 王领李萌月原勤轩张琳琳刘佳伟
Owner QILU UNIV OF TECH
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