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Preparation method of bifida ferment lysate

A fermentation product, bifid yeast technology, which is applied in the dissolution of microorganisms, skin care preparations, pharmaceutical formulations, etc., can solve the problem of reducing the activity of free radical scavenging substances, so as to improve the free radical scavenging rate, improve the preparation efficiency, and ensure The effect of scavenging free radical substance activity

Inactive Publication Date: 2020-07-17
济南碧蒙萱生物技术有限公司
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Problems solved by technology

[0004] In order to overcome the above-mentioned technical problems, the present invention provides a method for preparing a bifid yeast fermentation product lysate, which can solve the problem of reduced activity of free radical scavenging substances in the production process of bifid yeast fermentation product lysate

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  • Preparation method of bifida ferment lysate
  • Preparation method of bifida ferment lysate
  • Preparation method of bifida ferment lysate

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Embodiment Construction

[0016] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples.

[0017] This embodiment provides a method for preparing a bifid yeast fermentation product lysate, comprising the following steps:

[0018] S1: Boil 2% soybeans and 1% caramel malt in purified water for 2 hours, cool to room temperature, filter through a 800-mesh sieve, add 6% glucose, 1% yeast powder, 0.2% dipotassium hydrogen phosphate, and sterilized at 115°C for 20 minutes;

[0019] S2: After sterilization, cool to room temperature, inoculate with 3% bifida yeast liquid, and ferment at 37°C for 48 hours;

[0020] S3: The end point of fermentation is that the pH value of the fermentation broth is less than 4.0, if the pH value does not reach below 4.0 within 48 hours, continue to ferment;

[0021] S4: After reaching the end point of the fermentation, the fermentation li...

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Abstract

The invention discloses a preparation method of bifida ferment lysate, and relates to the technical field of related production of microbial cosmetic raw materials. The preparation method comprises the following steps: boiling 1-4% by mass of soybeans and 1-2% by mass of charred malt in purified water for 2 hours, performing cooling to room temperature, performing filtering by using an 800-mesh sieve, adding 5-10% by mass of glucose, 0.1-1% by mass of yeast extract powder and 0.1-0.5% by mass of dipotassium hydrogen phosphate, and performing sterilizing at 115 DEG C for 20 minutes; performingcooling to room temperature, inoculating 1.5-3.5% by mass of a bifida bacterium solution, and performing fermentation at 37 DEG C for 48 hours; centrifuging fermentation liquor to collect thalli afterfermentation is ended, and adding 0.2-2 times of sterile ultrapure water for redissolution; adding 0.1%-1.0% by mass of a lysozyme; adding 5% -20% by mass of trehalose as a protective agent, and performing thermal cracking at 80-100 DEG C; and performing cooling, and then performing normal temperature preservation. Therefore, the invention can solve the problem that activity of free radical scavenging substances is reduced in a production process of the bifida ferment lysate.

Description

technical field [0001] The invention relates to the technical field of production related to microbial cosmetic raw materials, in particular to a method for preparing a bifid yeast fermentation product lysate. Background technique [0002] Bifid yeast is one of the human intestinal flora, with strong intestinal adhesion and strong ability to colonize the intestinal tract, and has the functions of activating macrophages, stimulating the release of immune factors, maintaining the balance of intestinal flora, anti-tumor, and promoting Element absorption, control of endotoxin production and other important physiological health functions, so the application value of bifid yeast is extremely high. Bifida yeast fermentation product lysate is a widely used natural active cosmetic raw material. The raw material is cultured, inactivated and decomposed to obtain metabolites, cytoplasmic fragments, cell wall components and polysaccharide complexes; the raw material has strong It has an...

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Application Information

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IPC IPC(8): C12N1/16C12N1/06A61K8/9728A61Q19/08
CPCC12N1/16C12N1/063A61K8/9728A61Q19/08
Inventor 汤传忠潘信磊刘毅
Owner 济南碧蒙萱生物技术有限公司
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