Method for analyzing distribution and transportation routes of flavonoid compounds in exocarpium citri grandis
A technology for analyzing tangerine and chemical compounds, which is applied in the field of analyzing the distribution and transportation pathways of flavonoids in tangerine red, can solve the problems of inability to analyze distribution and transportation pathways, cell shrinkage and deformation, and low concentration of flavonoids, and achieves rapid and accurate histochemistry. The effect of localization, complete cellular framework structure, and precise histochemical localization
Inactive Publication Date: 2020-07-24
GUANGDONG UNIV OF PETROCHEMICAL TECH
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Problems solved by technology
[0003] The commonly used flavonoid staining method is to stain the tissue section with 5% NaOH solution, and it will turn yellow if it contains flavonoids. However, due to the nature of flavonoids that are easily soluble in alkaline solutions, flavonoids dissolve and diffuse in plant tissues, affecting localization. accuracy, making it impossible to resolve its distribution and transport routes
[0004] Freehand sections that have not been dried, directly add staining agent. On the one hand, due to the high water content of the tissue, the concentration of flavonoids is low; Poor; if the freehand section evaporates naturally or heats to evaporate the water, the result is that the cells shrink and deform, and the flavonoids are displaced in the tissue, and the result is that they cannot be located
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Embodiment 1
[0023] 1. Freehand slicing step: Pick a young leaf from the shoot with 5 leaves, cut off a small stem section on the shoot, carefully insert them into fresh potato cubes and fix them, and slice them by hand with a double-sided blade. The young fruit grown for 15 days was vertically and longitudinally cut into four pieces, and one of them was sliced by hand to obtain slices of leaves, stems and fruits.
[0024] 2. Freeze-drying and slicing steps:
[0025] (1) Select uniform and transparent slices of leaves, stems and fruits, place them on glass slides, and cover with coverslips;
[0026] (2) Pre-cool the sections covered with coverslips in the cold trap of the freeze dryer for 3 hours, and set the temperature to -40°C;
[0027] (3) Move the slices to the drying oven, and vacuumize when the temperature is lower than -40°C;
[0028] (3) When the pressure reaches 10Pa, turn on the heating system, the heating temperature is 45°C, the vacuum degree in the drying oven is controll...
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The invention discloses a method for analyzing distribution and transportation paths of flavonoid compounds in exocarpium citri grandis. The method comprises a free-hand slicing step, a freeze-dryingslicing step, a dyeing step by using a magnesium acetate solution and a observation step by using a fluorescence microscope. The method is simple to operate, is complete in cell framework structure, is free of deformation, is accurate in histochemical positioning, and has application significance in researching flavone metabolic pathways in plants and production and quality control of exocarpium citri grandis medicinal materials.
Description
technical field [0001] The invention belongs to the field of phytochemical components, and in particular relates to a method for analyzing the distribution and transport route of flavonoids in Huajuhong. Background technique [0002] Huajuhong is a precious traditional Chinese medicine for resolving phlegm and relieving cough. At present, it has been included in the prescription medicine for the diagnosis and treatment of new coronary pneumonia. The main medicinal ingredients of Huajuhong are flavonoids. Flavonoids have the functions of scavenging free radicals, anti-inflammation, and preventing cardiovascular diseases. They have physiological functions such as anti-aging and lowering blood lipids. They have broad applications in the fields of health care products, medicine, and food. prospect. Although there are many studies on the identification and content of flavonoids in different organs and different parts of Huajuhong, there is a lack of research on the distribution ...
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Login to View More IPC IPC(8): G01N1/28G01N1/30G01N21/64
CPCG01N1/28G01N1/30G01N21/6402G01N21/6486
Inventor 韩寒冰黄新敏何勤勤马超刘杰凤
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH