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Preparation method and application of colorimetric array sensor adopting zirconium-based metal organic framework and single-stranded DNA modified gold nanoparticles

A gold nanoparticle and array sensor technology, applied in the field of analytical chemistry, can solve the problem that the detection system cannot perform multiple analyzes at the same time, and achieve the effects of low production cost, easy operation, and stable reaction system

Active Publication Date: 2020-07-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many assay systems cannot be multiplexed simultaneously due to the limitations of specific binding interactions between proteins and receptors

Method used

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  • Preparation method and application of colorimetric array sensor adopting zirconium-based metal organic framework and single-stranded DNA modified gold nanoparticles
  • Preparation method and application of colorimetric array sensor adopting zirconium-based metal organic framework and single-stranded DNA modified gold nanoparticles
  • Preparation method and application of colorimetric array sensor adopting zirconium-based metal organic framework and single-stranded DNA modified gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: Detection of various proteins using a colorimetric array sensor interacting with zirconium-ion metal-organic frameworks and single-stranded DNA-modified gold nanoparticles

[0039] Step 1: Preparation of zirconium ion metal-organic framework: ZrCl 4 (120 mg), aminoterephthalic acid (110 mg) and benzoic acid (1.9 g) were dissolved in 10 mL of DMF and transferred to a Teflon liner. After ultrasonic treatment for 3 minutes, put it in 120°C and let it stand for 24 hours to react. The resulting product was obtained by centrifugation and washed repeatedly three times with fresh DMF and methanol (8,000 rpm, 5 minutes). Finally, to remove the residual organic solvent, the nanoparticles were collected after drying overnight at 65 °C.

[0040] Step 1: Preparation of gold nanoparticles: When the chloroauric acid solution (50 mL, 1 mM) was heated to boiling, trisodium citrate solution (5 mL, 38.8 mM) was quickly added under vigorous stirring. Continue to heat the mixed ...

Embodiment 2

[0044] Example 2: Detection of a variety of semen samples using a colorimetric array sensor interacting with zirconium-ion metal-organic frameworks and single-stranded DNA-modified gold nanoparticles

[0045] Step 1: Preparation of zirconium ion metal-organic framework: ZrCl 4 (120 mg), aminoterephthalic acid (110 mg) and benzoic acid (1.9 g) were dissolved in 10 mL of DMF and transferred to a Teflon liner. After ultrasonic treatment for 3 minutes, put it in 120°C and let it stand for 24 hours to react. The resulting product was obtained by centrifugation and washed repeatedly three times with fresh DMF and methanol (8,000 rpm, 5 minutes). Finally, to remove the residual organic solvent, the nanoparticles were collected after drying overnight at 65 °C.

[0046] Step 1: Preparation of gold nanoparticles: When the chloroauric acid solution (50 mL, 1 mM) was heated to boiling, trisodium citrate solution (5 mL, 38.8 mM) was quickly added under vigorous stirring. Continue to hea...

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Abstract

The invention relates to a preparation method and application of a colorimetric array sensor adopting a zirconium-based metal organic framework and single-stranded DNA modified gold nanoparticles. Sixdifferent single-stranded DNA sequences are designed to modify the surfaces of gold nanoparticles, so that unique sensor recognition elements are formed. Through different intermolecular forces, thenovel material, namely, the zirconium-based metal organic framework can be combined with or precipitate the gold nanoparticles modified by the single-stranded DNA sequences, after some protein or semen samples are added, the binding force can be accelerated or slowed down, the aggregation behavior is influenced, and thus a detectable signal is generated. Therefore, the recognition elements have different interactions when binding with different targets, and by combined analysis of the colorimetric response of each recognition element, different targets can be detected obviously. The colorimetric array sensor can be simply and efficiently used for colorimetric array analysis of various proteins and male seminal fluid, and a detection basis is provided for analysis of male infertility.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a preparation method and application of a colorimetric sensor of zirconium ion metal-organic framework and single-stranded DNA modified gold nanoparticles [0002] Background of the invention [0003] Male infertility is a social disease that plagues male compatriots. Rapid and accurate identification of sperm motility is the key to diagnosis and formulation of targeted treatment strategies. In addition to known factors, there are still 30-45% of patients whose semen parameters are abnormal and the cause has not been found out. These abnormal cases are collectively referred to as idiopathic male infertility. Thresholds for normal ranges have not been clearly defined due to the lack of available data on the most direct samples of semen variables. Moreover, because of the complexity and diversity of samples, it brings many limitations to the detection. Detection of the proteome...

Claims

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Application Information

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IPC IPC(8): G01N21/31C08G83/00
CPCG01N21/31C08G83/008
Inventor 李根喜孙召伟吴帅马洁桦潘艳红韩祎巍
Owner NANJING UNIV
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