Impatiens balsamina glycan, extraction method thereof and application of impatiens balsamina glycan in preparation of anti-SARS-CoV-2 drugs

An extraction method and a technique for impatiens, applied in the field of medicine

Active Publication Date: 2020-07-28
爱礼康生物医药(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report that the glycans of impatiens can be used for anti-SARS-CoV-2

Method used

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  • Impatiens balsamina glycan, extraction method thereof and application of impatiens balsamina glycan in preparation of anti-SARS-CoV-2 drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: a kind of extraction method of impatiens glycan, comprises the following steps:

[0024] Step 1, pulverizing the dried Impatiens plant to obtain Impatiens powder;

[0025] Step 2: Add 20 times the mass of deionized water to the impatiens powder to obtain a mixed solution, heat the mixed solution to 58°C, and then add cellulase, amylase and proteolytic enzyme for enzymatic hydrolysis for 1.5 hours to obtain enzymatic hydrolysis Solution; wherein, the addition of cellulase, amylase and proteolytic enzyme is respectively 3.2%, 1.2%, 0.5% of the quality of Impatiens powder;

[0026] Step 3, heating the enzymolysis solution to 105°C and keeping it for 1.2h, then cooling and filtering to obtain the filtrate;

[0027] Step 4, heating and concentrating the filtrate to obtain a concentrated solution, putting the concentrated solution into a dialysis bag, and dialysis treating the concentrated solution in the dialysis bag with running water for 2 days to obtain a d...

Embodiment 2

[0030] Embodiment 2: a kind of extracting method of impatiens glycan, comprises the following steps:

[0031] Step 1, pulverizing 1 kg of dried Impatiens plants to obtain Impatiens powder;

[0032] Step 2: Add 22 times the mass of deionized water to the impatiens powder to obtain a mixed solution, heat the mixed solution to 50°C, and then add cellulase, amylase and proteolytic enzyme for enzymatic hydrolysis for 1.2 hours to obtain enzymatic hydrolysis Solution; wherein, the addition of cellulase, amylase and proteolytic enzyme is respectively 3%, 1.3%, 0.4% of the quality of Impatiens powder;

[0033] Step 3, heating the enzymolysis solution to 110°C and keeping it for 1 hour, then cooling and filtering to obtain the filtrate;

[0034] Step 4, heating and concentrating the filtrate to obtain a concentrated solution, putting the concentrated solution into a dialysis bag, and dialyzing the concentrated solution in the dialysis bag for 3 days by running water to obtain a dialys...

Embodiment 3

[0037] Embodiment 3: a kind of extraction method of impatiens glycan, comprises the following steps:

[0038] Step 1, pulverizing the dried Impatiens plant to obtain Impatiens powder;

[0039] Step 2: Add 18 times the mass of deionized water to the impatiens powder to obtain a mixed solution, heat the mixed solution to 60°C, and then add cellulase, amylase and proteolytic enzyme for enzymatic hydrolysis for 1 hour to obtain an enzymatic hydrolysis solution Wherein, the addition amount of cellulase, amylase and proteolytic enzyme is respectively 2.8%, 1%, 0.6% of the mass of Impatiens powder;

[0040] Step 3, heating the enzymolysis solution to 100°C and keeping it for 1.1h, then cooling and filtering to obtain the filtrate;

[0041] Step 4, heat and concentrate the filtrate to obtain a concentrated solution, put the concentrated solution into a dialysis bag, and dialyze the concentrated solution in the dialysis bag by running water for 2.5 days to obtain a dialysis solution; ...

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Abstract

The invention discloses an extraction method of impatiens balsamina glycan and an application of the impatiens balsamina glycan in preparation of an anti-SARS-CoV-2 drug, and belongs to the technicalfield of medicines. The extraction method comprises the following steps 1, crushing dried impatiens balsamina plants to obtain impatiens balsamina powder, 2, adding deionized water of which the mass is 18-22 times that of the impatiens balsamina powder into the impatiens balsamina powder to obtain a mixed solution, heating the mixed solution, and adding cellulase, amylase and proteolytic enzyme for enzymolysis for 1-1.5 hours to obtain an enzymolysis solution, 3, heating the enzymolysis solution to 100-110 DEG C, keeping the temperature for 1-1.2 hours, and then cooling and filtering to obtaina filtrate, 4, heating and concentrating the filtrate to obtain a concentrated solution, and then dialyzing for 2-3 days to obtain a dialysate, 5, heating, concentrating and centrifuging the dialysate, removing precipitates, adding 95% ethanol with the volume 5-6 times that of the supernatant, standing overnight, and carrying out alcohol precipitation to obtain an alcohol precipitation solution,and 6, carrying out centrifugal treatment on the alcohol precipitation solution to obtain precipitates, and alternately washing the precipitates with absolute ethyl alcohol and acetone for 3-5 times to obtain the washed precipitates, namely impatiens balsamina glycan.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an impatiens glycan, an extraction method and its application in preparing anti-SARS-CoV-2 medicines. Background technique [0002] 2019 Novel Coronavirus, also known as SARS-CoV-2. Common signs of human infection with SARS-CoV-2 include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. Currently, there is no specific treatment for the disease caused by the novel coronavirus. [0003] The new coronavirus pneumonia virus SARS-Cov-2 is a typical coronavirus 2-5, and the diameter of the virus particle is about 60-220nm. There are three kinds of glycoproteins on the membrane surface: spike glycoprotein (S, Spike Protein), small envelope glycoprotein (E, Envelope Protein) and membrane glycoprotein (M, Membrane Protein). Among th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/14
CPCC08B37/0003C08B37/00A61K31/715A61P31/14
Inventor 丁侃陈霞孙成新姚晓东王秋雨张艳丽
Owner 爱礼康生物医药(苏州)有限公司
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