An immunomodulatory microsphere preparation targeting tumor-associated macrophages and its preparation method and application

Abstract

The invention discloses an immunoregulation microsphere preparation targeting tumor-associated macrophages (TAMs) as well as a preparation method and application of the immunoregulation microsphere preparation. The microsphere preparation is a double-targeting preparation binding macrophage targeting peptide and B cell lymphoma 6 (Bcl6) factor inhibiting peptide; and the microsphere preparation isprepared as follows: the Bcl6 factor inhibiting peptide is supported on a liposome to obtain a Bcl6 factor inhibiting peptide-liposome solution; and then, the Bcl6 factor inhibiting peptide-liposomesolution is bound with the macrophage targeting peptide. The macrophage targeting peptide and an intracellular signal molecule inhibitor are bound for the first time and supported on nano microspheresto prepare the immune preparation directionally transferred to a tumor microenvironment. Besides, the preparation is found to be able to significantly reduce the proportion and number of macrophagesin lung cancer tumor and reverse stem cell-like phenotypes of the macrophages for the first time, and the preparation can significantly inhibit the size and weight of the lung cancer tumor and has a remarkable antitumor function.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an immunoregulatory microsphere preparation targeting tumor-associated macrophages, a preparation method thereof, and an application in the preparation of anti-tumor products. Background technique [0002] Tumor is currently a global public health problem and a major threat to human health. Its morbidity and mortality are increasing year by year. Slowing down or inhibiting the progression of tumors has become the main goal of current cancer treatment. Existing studies have confirmed that the formation and development of tumors are closely related to the tumor microenvironment (tumor microenvironment, TME). TME not only provides the necessary nutrients, growth conditions and physical substrates for tumor growth, but also transforms and domesticates the immune cells infiltrating it, such as lymphocytes and macrophages, so that they undergo phenotype, functional tran...

AI Technical Summary

Problems solved by technology

For example, many therapeutic methods based on TAMs clearance lack tumor targeting and have low permeability to the tumor microenvironment, resulting in poor curative effect.

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