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Body fluid tumor cell DNA extraction kit

A tumor cell and kit technology, applied in the fields of biotechnology and biomedicine, can solve the problems of tumor cell DNA difficulty, low concentration, and DNA analysis difficulty, and achieve good detection effect and accurate detection results

Pending Publication Date: 2020-08-04
猎源(上海)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the low concentration of tumor cells in body fluids, it is very difficult to extract the DNA of tumor cells in body fluids
Even if you barely use the kit to extract, the obtained DNA mainly includes the DNA of normal cells, which brings great difficulties to the subsequent DNA analysis of body fluid tumor cells

Method used

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  • Body fluid tumor cell DNA extraction kit
  • Body fluid tumor cell DNA extraction kit
  • Body fluid tumor cell DNA extraction kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This example provides a specific immunomagnetic bead and its preparation method.

[0022] The specific immunomagnetic beads in this example are magnetic nanospheres modified with epithelial cell adhesion molecule (EpCAM).

[0023] The preparation process of the specific immunomagnetic beads in this embodiment is:

[0024] Step S1-1, weigh 10 mg each of dimethyloctadecylglycidyl ammonium chloride (GHDC) and cholesterol, mix GHDC and cholesterol to obtain the first mixture, and add the first mixture to the mill in a pear-shaped bottle.

[0025] Step S1-2, measure 1.0mL of the magnetic bead solution after removing ethanol and dissolve it in 2.0mL CH 2 Cl 2 , the first solution was obtained.

[0026] Step S1-3, pouring the first solution into a ground-mouth pear-shaped bottle so that the first solution and the first mixture are mixed to obtain a second solution. At this time, the volume-to-mass ratio of the second solution:mixture=3:20.

[0027] Step S1-4, use a pipet...

Embodiment 2

[0032] This example provides a specific immunomagnetic bead and its preparation method.

[0033] The specific immunomagnetic beads in this example are magnetic nanospheres co-modified by epithelial cell adhesion molecule (EpCAM), epidermal growth factor receptor antibody (EGFR) and vimentin.

[0034] The preparation process of the specific immunomagnetic beads in this embodiment is:

[0035] Step S2-1, weighing 10 mg each of dimethyloctadecylglycidyl ammonium chloride (GHDC) and cholesterol, mixing GHDC and cholesterol to obtain the first mixture, and adding the first mixture to the mill in a pear-shaped bottle.

[0036] Step S2-2, measure 1.0mL of the magnetic bead solution after removing ethanol and dissolve it in 2.0mL CH 2 Cl 2 , the first solution was obtained.

[0037] Step S2-3, pouring the first solution into a ground-mouth pear-shaped bottle so that the first solution and the first mixture are mixed to obtain a second solution. At this time, the volume-to-mass ra...

Embodiment 3

[0043] This embodiment provides a kit for extracting tumor cell DNA from body fluid.

[0044] The body fluid tumor cell DNA extraction kit in this example is used to extract the DNA of tumor cells that have escaped from solid tumors and entered body fluid, including specific immunomagnetic beads, magnetic separation frame, lysate, first washing solution, and second washing solution , eluent, DNA adsorption column and DNA preservation tube.

[0045] The specific immunomagnetic beads in this example are EpCAM immunomagnetic beads prepared according to the method in Example 1.

[0046] The magnetic separation frame is made of plastic material through injection molding process and contains permanent magnets. When in use, the magnetic separation rack can magnetically separate the specific immunomagnetic beads and other solutions (such as body fluids) through the attraction of the magnet to the magnetic beads.

[0047] The lysate is used to lyse the captured body fluid tumor cells...

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Abstract

The invention provides a body fluid tumor cell DNA extraction kit. The body fluid tumor cell DNA extraction kit is used for extracting DNA of tumor cells which are separated from solid tumors and enter body fluid, and is characterized by comprising specific immunomagnetic beads, a magnetic separation frame, a lysis solution, a first washing liquid, a second first washing liquid and an eluant, wherein the specific immunomagnetic beads are used for capturing the body fluid tumor cells from the body fluid; the magnetic separation frame is used for attracting the specific immunomagnetic beads through magnetic force; the lysis solution is used for carrying out cell lysis on the captured body fluid tumor cells; the first washing liquid is used for washing the lysed body fluid tumor cells for thefirst time; the second washing liquid is used for washing the body fluid tumor cells subjected to the first washing for the second time; and the specific immunomagnetic beads are magnetic nano microspheres modified by epithelial cell adhesion molecules.

Description

technical field [0001] The invention relates to the fields of biotechnology and biomedicine, in particular to a kit for extracting body fluid tumor cell DNA. Background technique [0002] DNA, translated into Chinese as deoxyribonucleic acid, is the main component of chromosomes. DNA is the most important biological information molecule, which can form genetic instructions to guide the development of organisms and the operation of life functions. Transcribing genes and expressing proteins in an orderly manner in time and space completes all programs of directional development; preliminarily determines the unique traits and personalities of organisms and all stress responses when interacting with the environment. In addition to playing a very important role in the normal growth, development and reproduction of organisms and other life activities, it is also closely related to abnormal conditions of life, such as tumor occurrence, radiation damage, and genetic diseases. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/533G01N33/574
CPCG01N33/54326G01N33/54346G01N33/533G01N33/57484
Inventor 梁晓飞宋萍
Owner 猎源(上海)生物医药科技有限公司
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