Method for obtaining regenerated plants from common camellia oleifera in somatic embryogenesis mode
A technology of embryogenesis and regeneration of plants, applied in the field of plant cell engineering, can solve the problems of low plant regeneration efficiency, self-sufficiency rate of less than 40%, affecting grain and oil safety, etc., and achieve the effect of more normal regeneration plants, good social and economic benefits
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[0034] Example
[0035] The experimental steps are as follows:
[0036] In mid-July, good individual tea fruits were selected from mature camellia forests in Meihuashan Village, Shunhe Town, Macheng, Hubei, and stored in a refrigerator at 4°C. The sterilization inoculation was completed within 3 days.
[0037] Under the aseptic condition of the ultra-clean workbench, peel off the peel of Camellia oleifera fruit with your bare hands, disinfect the surface in 75% alcohol for 2 minutes, then disinfect with 0.1% HgCl2 for 10 minutes, rinse with sterile water 4 times, and peel off the inside Seed coat. Under aseptic conditions, the immature embryos were cut into pieces, and each immature embryo was cut into 3-5 pieces and inoculated into 90mm petri dishes (callus induction medium), and 7-9 explants were inoculated per dish.
[0038] The medium used for callus induction (callus induction medium) is a modified MSB medium supplemented with plant hormones. The composition of MSB medium is: po...
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