Method for obtaining regenerated plants from common camellia oleifera in somatic embryogenesis mode

Abstract

The invention provides a method for obtaining regenerated plants from common camellia oleifera in a somatic embryogenesis mode. The method comprises the following steps of storing young embryos of thecommon camellia oleifera in the middle ten days of July at 4 DEG C, cutting the young embryos into blocks after disinfection, and inoculating the young embryos into a callus induction culture mediumfor callus induction culture; selecting well-growing calluses, and subculturing the calluses in a differential culture medium for embryoid differentiation; transferring explants with embryoids into anembryoid development and plant regeneration culture medium for embryoid development and plant regeneration; transferring the embryoids with obvious cotyledons to a rooting culture medium for rootingculture; and finally, domesticating and transplanting obtained complete plants into a nutritional bowl for continuous growth. According to the method provided by the invention, the common camellia oleifera can be subjected to somatic embryogenesis within 6 months to obtain the regenerated plants, a convenient, rapid and effective method is provided for obtaining the regenerated plants through thesomatic embryogenesis of the common camellia oleifera, and the method has relatively good social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and in particular relates to a method for obtaining regenerated plants of common camellia oleifera through somatic embryogenesis. Background technique [0002] Camellia oleifera is a general term for species with high seed oil content in the Camellia genus, and it is also a unique woody oil tree species in my country. Camellia oleifera, olive, oil palm, and coconut are also known as the world's four major woody oil crops. The unsaturated fatty acid content of camellia oil is as high as 90%, which is much higher than that of vegetable oil, peanut oil and soybean oil. The vitamin E content of camellia oil is twice as high as that of olive oil, and it contains specific physiologically active substances such as camellia glucoside, which has extremely high nutritional value. At the same time, camellia oleifera forest is a good ecological forest, and camellia oleifera is also an importa...

AI Technical Summary

Problems solved by technology

In addition, my country's edible oil consumption is huge, but the current self-sufficiency rate is less than 40%, which seriously affects my country's grain and oil security

[0003] With the development of production and the continuous improvement of social needs, the production has put forward higher and higher requirements for the yield, quality, and resistance of Camellia oleifera varieties. The solution to these problems can no longer be met by relying on traditional breeding methods.

So far, some progress has been made in the tissue culture of camellia oleifera, but a genetic transformation technology system based on somatic embryogenesis has not been established, which is related to the low efficiency of plant regeneration in camellia oleifera through somatic embryogenesis

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