Method for obtaining regenerated plants from common camellia oleifera in somatic embryogenesis mode
A technology of embryogenesis and regeneration of plants, applied in the field of plant cell engineering, can solve the problems of low plant regeneration efficiency, self-sufficiency rate of less than 40%, affecting grain and oil safety, etc., and achieve the effect of more normal regeneration plants, good social and economic benefits
Active Publication Date: 2020-08-07
HUANGGANG NORMAL UNIV
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The invention provides a method for obtaining regenerated plants from common camellia oleifera in a somatic embryogenesis mode. The method comprises the following steps of storing young embryos of thecommon camellia oleifera in the middle ten days of July at 4 DEG C, cutting the young embryos into blocks after disinfection, and inoculating the young embryos into a callus induction culture mediumfor callus induction culture; selecting well-growing calluses, and subculturing the calluses in a differential culture medium for embryoid differentiation; transferring explants with embryoids into anembryoid development and plant regeneration culture medium for embryoid development and plant regeneration; transferring the embryoids with obvious cotyledons to a rooting culture medium for rootingculture; and finally, domesticating and transplanting obtained complete plants into a nutritional bowl for continuous growth. According to the method provided by the invention, the common camellia oleifera can be subjected to somatic embryogenesis within 6 months to obtain the regenerated plants, a convenient, rapid and effective method is provided for obtaining the regenerated plants through thesomatic embryogenesis of the common camellia oleifera, and the method has relatively good social benefits and economic benefits.
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