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Method for promoting germination of Taxillus chinensis (DC.) Danser seeds

A technology of mulberry parasitism and seeds, applied in the fields of seed and rhizome treatment, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of slow germination, reduction of wild resources, and influence on host growth, etc., to improve germination Uniformity, shortening germination time, and solving the effect of slow germination

Active Publication Date: 2020-08-14
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, mulberry medicinal materials are mainly wild resources. On the one hand, because it parasitizes on forests, fruit trees and other economic forests and affects the growth of the host, people have to get rid of it, resulting in a decrease in its number. On the other hand, mulberry fruit It is a berry. In the wild state, its propagation and reproduction are mainly realized by some carnivorous birds swallowing the fruit, digesting the pulp, expelling the seeds and sticking to the host to survive. However, this method has high requirements on the external environmental conditions, and the germination rate is low and the germination is slow. As a result, the seedling rate is low and wild resources are greatly reduced. Therefore, in order to meet market demand and ensure the quality of medicinal materials, standardized cultivation will be an inevitable choice
[0003] However, due to the intolerant dehydration and sensitivity to low temperature of mulberry parasitic seeds, they germinate slowly, irregularly, and low germination rate in the natural state of the laboratory, and there is no report on promoting the germination of mulberry parasitic seeds in the prior art

Method used

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  • Method for promoting germination of Taxillus chinensis (DC.) Danser seeds
  • Method for promoting germination of Taxillus chinensis (DC.) Danser seeds

Examples

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Embodiment 1

[0025] A method for promoting the germination of mulberry seeds. Soak the mulberry seeds with a seed soaking agent for 5 minutes, then put the mulberry seeds into a culture dish with filter paper, and place them in a constant temperature culture at a temperature of 20°C and a humidity of 75%. In case, germination is cultivated to seed germination, and the kinetin that contains the dinitrophenolate sodium of 5mg / L, the diethylhexanoate of 5mg / L and the kinetin of 2mg / L in the described seed soaking agent.

[0026] In the method for promoting the germination of mulberry seeds, before soaking the mulberry seeds with a seed soaking agent, the mulberry seeds are disinfected, and the specific steps are as follows: the selected mulberry seeds are placed in a 0.1% mass fraction Soak in the aqueous solution of detergent for 5 minutes, then soak in the aqueous solution of absolute ethanol with a mass fraction of 75% for 5 minutes, and wash with sterile water for 3 times.

[0027] The se...

Embodiment 2

[0030] A method for promoting the germination of mulberry seeds. Soak the mulberry seeds with a seed soaking agent for 50 minutes, then put the mulberry seeds into a petri dish with filter paper, and place them in a constant temperature culture at a temperature of 32°C and a humidity of 85%. In case, germination is cultivated to seed germination, and the described seed soaking agent contains the dinitrophenolate sodium of 30mg / L, the diethyl diethylhexanoate of 50mg / L and the kinetin of 20mg / L.

[0031] In the method for promoting the germination of mulberry seeds, before soaking the mulberry seeds with a seed soaking agent, the mulberry seeds are disinfected, and the specific steps are as follows: the selected mulberry seeds are placed in a 0.1% mass fraction Soak in detergent solution for 10 minutes, then soak in 75% absolute ethanol solution for 10 minutes, and wash with sterile water for 4 times.

[0032] The selection specifically refers to harvesting the mulberry parasit...

Embodiment 3

[0035] A method for promoting the germination of mulberry seeds. Soak the mulberry seeds with a seed soaking agent for 30 minutes, then put the mulberry seeds into a petri dish with filter paper, and place them in a constant temperature culture with a temperature of 25°C and a humidity of 80%. In case, germination is cultivated to seed germination, and the kinetin that contains the dinitrophenolate sodium of 15mg / L, the diethyl diethylhexanoate of 10mg / L and the kinetin of 12mg / L in the described seed soaking agent.

[0036] In the method for promoting the germination of mulberry seeds, before soaking the mulberry seeds with a seed soaking agent, the mulberry seeds are disinfected, and the specific steps are as follows: the selected mulberry seeds are placed in a 0.1% mass fraction Soak in the aqueous solution of detergent for 7 minutes, then soak in the aqueous solution of absolute ethanol with a mass fraction of 75% for 7 minutes, and wash with sterile water for 3 times.

[...

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Abstract

The invention discloses a method for promoting the germination of Taxillus chinensis (DC.) Danser seeds. The method comprises the following steps: soaking the Taxillus chinensis (DC.) Danser seeds for5-50 min with a seed-soaking agent, then placing the Taxillus chinensis (DC.) Danser seeds into a petri dish with filter paper, and placing the petri dish into a constant-temperature incubator with atemperature of 20-32 DEG C and a humidity of 75-85% for germination culture until seed germination, wherein the seed-soaking agent contains 5-30 mg / L of sodium nitrophenolate, 5-50 mg / L of 2-diethylaminoethyl hexanoate and 2-20 mg / L of kinetin. The method of the invention significantly promotes the germination of the Taxillus chinensis (DC.) Danser seeds in the constant-temperature incubator through the soaking treatment with the seed-soaking agent, and solves the problem of slow germination and low germination rate of the Taxillus chinensis (DC.) Danser seeds in the natural state.

Description

technical field [0001] The invention relates to the technical field of plant seed germination. More specifically, the present invention relates to a method for promoting the germination of mulberry parasitic seeds. Background technique [0002] Taxillus chinensis (DC.) Danser, a traditional Chinese herbal medicine commonly used in large quantities, has the effects of dispelling rheumatism, nourishing the liver and kidney, strengthening bones and bones, and soothing the fetus. herbs. At present, mulberry medicinal materials are mainly wild resources. On the one hand, because it parasitizes on forests, fruit trees and other economic forests and affects the growth of the host, people have to get rid of it, resulting in a decrease in its number. On the other hand, mulberry fruit It is a berry. In the wild state, its propagation and reproduction are mainly realized by some carnivorous birds swallowing the fruit, digesting the pulp, expelling the seeds and sticking to the host t...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N43/90A01N37/12A01N33/22A01P21/00
CPCA01C1/00A01N33/22A01N37/12A01N43/90
Inventor 潘丽梅韦树根付金娥万凌云
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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