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Freeze-drying protective agent and application thereof to freeze-drying of bifidobacterium adolescentis

A technology of freeze-drying protective agent and bifidobacteria, which is applied in the field of microorganisms, can solve the problems of unsatisfactory effect and freeze-drying survival rate of only 57.6%, and achieve the effect of improving freeze-drying survival rate

Active Publication Date: 2020-08-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, currently there are few lyoprotectants specially developed for the probiotic Bifidobacterium adolescentis, and most of the effects are not ideal. For example, the literature "Xu Haiyan, Zhang Zhiyan, Cao Bin, et al. Optimizing the culture medium and the screening of lyoprotectant[J]. Animal Husbandry and Feed Science, 2013,34(3):15-18." In Zhang Zhiyan et al., they used lyoprotectant containing trehalose, skimmed milk powder, glycerin, sucrose and gelatin. When freeze-drying Bifidobacterium adolescentis, the survival rate of freeze-drying is only 57.6%; in the literature "Bai Wen. Research on high-density culture method and freeze-drying process of Bifidobacterium adolescentis [D]. Jilin Agricultural University, 2006.", Bai Wen et al. used a lyoprotectant containing skimmed milk, sucrose and mannitol to lyophilize Bifidobacterium adolescentis, and the lyophilized survival rate was only 73%.

Method used

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  • Freeze-drying protective agent and application thereof to freeze-drying of bifidobacterium adolescentis
  • Freeze-drying protective agent and application thereof to freeze-drying of bifidobacterium adolescentis
  • Freeze-drying protective agent and application thereof to freeze-drying of bifidobacterium adolescentis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of Bifidobacterium adolescentis freeze-dried powder (Bifidobacterium adolescentis GDMCC No.60706)

[0033] Specific steps are as follows:

[0034] Formulate lyoprotectants 1 to 4 according to the formula in Table 1; use an inoculation loop to dip the Bifidobacterium juvenile GDMCC No.60706 bacteria solution in the bacteria preservation tube, streak on the MRS solid medium, and culture at 37°C for 36 hours to obtain single Bacterial colonies; pick a single colony and inoculate it in MRS liquid medium, and incubate at 37°C for 12 hours to obtain seed liquid; inoculate the seed liquid into fresh MRS liquid medium at 2% (v / v) inoculum size, and inoculate at 37°C Cultivate at constant temperature for 14 hours to the end of logarithmic growth to obtain the bacterial liquid; centrifuge the bacterial liquid at 8000 g for 20 min to collect the bacterial sludge; wash the bacterial sludge twice with sterile saline and resuspend in lyoprotectant 1-4 to obtain...

Embodiment 2

[0042] Embodiment 2: Preparation of Bifidobacterium adolescentis freeze-dried powder

[0043] Specific steps are as follows:

[0044] On the basis of Example 1, Bifidobacterium adolescentis GDMCC No.60706 was replaced with Bifidobacterium adolescentis GDMCC No.60707 to obtain Bifidobacterium adolescentis freeze-dried powders 5-8.

[0045] Detect the viable count of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 5~8, and calculate the freeze-dried survival rate of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 5~8 (detection results are shown in Table 3 ).

[0046] It can be seen from Table 3 that the freeze-dried survival rate of Bifidobacterium adolescentis in the freeze-dried powder of Bifidobacterium adolescentis prepared by using the freeze-drying protectant containing 37.5% sorbitol, 37.5% raffinose and 25% whey protein is the highest , up to 92.45±4.81%.

[0047] The freeze-dried survival rate of Bifi...

Embodiment 3

[0049] Embodiment 3: Preparation of Bifidobacterium adolescentis freeze-dried powder

[0050] Specific steps are as follows:

[0051] On the basis of Example 1, Bifidobacterium adolescentis GDMCC No.60706 was replaced with Bifidobacterium adolescentis GDMCC No.14395 to obtain Bifidobacterium adolescentis freeze-dried powders 9-12.

[0052] Detect the viable count of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 9~12, and calculate the freeze-dried survival rate of Bifidobacterium adolescentis in Bifidobacterium adolescentis freeze-dried powder 9~12 (detection results are shown in Table 4 ).

[0053] As can be seen from Table 4, the freeze-dried survival rate of Bifidobacterium adolescentis in the Bifidobacterium adolescentis freeze-dried powder prepared by using the freeze-drying protectant containing 37.5% sorbitol, 37.5% raffinose and 25% whey protein is the highest , up to 95.72±4.01%.

[0054] The freeze-dried survival rate of Bifidoba...

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Abstract

The invention discloses a freeze-drying protective agent and an application thereof to freeze-drying of bifidobacterium adolescentis, belongs to the technical field of microorganisms, and provides thefreeze-drying protective agent capable of obviously increasing the freeze-drying survival rate of the bifidobacterium adolescentis. The freeze-drying protective agent comprises the components of sorbitol, raffinose and whey protein; and when lyophilized powder of the bifidobacterium adolescentis is prepared by use of the freeze-drying protective agent, the freeze-dried survival rate of the bifidobacterium adolescentis is as high as 97.43 plus or minus 4.98%.

Description

technical field [0001] The invention relates to a freeze-drying protectant and its application in freeze-dried Bifidobacterium adolescentis, belonging to the technical field of microorganisms. Background technique [0002] Bifidobacterium adolescentis is a kind of probiotics. It has rich probiotic functions, including treating chronic diarrhea, constipation, anti-aging, lowering cholesterol, preventing steatohepatitis, relieving colitis, and inhibiting periodontitis. Due to its remarkable probiotic effect, Bifidobacterium adolescent has increasingly become the focus of people's research, development and production. At present, Bifidobacterium adolescentis has been widely used in food, medicine and other fields. [0003] Bifidobacterium adolescentis freeze-dried powder is the most common Bifidobacterium adolescentis product on the market. This product is mainly obtained by freeze-drying Bifidobacterium adolescentis. Control and other advantages. In the process of preparing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C12R1/01
CPCC12N1/04C12N1/20Y02A50/30
Inventor 马申嫣崔树茂蒋文鑫陆文伟翟齐啸唐鑫毛丙永赵建新陈卫
Owner JIANGNAN UNIV
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