Quantitative detection kit for ovarian cancer marker CA125
A CA125, quantitative detection technology, applied in the biological field, can solve problems such as low sensitivity, false positives, and ELISA is easily interfered by external factors, and achieve the effect of shortening detection time, achieving stability, high sensitivity and accuracy
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Embodiment 1
[0031] Example 1: Preparation of the first complex
[0032] First, take 300 μL of commercially purchased mercapto silica nanospheres (average particle size about 90 nm) and centrifuge to precipitate, after drying, add 3 mL of commercially purchased CdSe (cadmium selenide) quantum dots (average particle size about 3.5 nm) sonicated for 15 min until the solution became clear. Then, the solution was centrifuged at 10000 rpm for 6 min, and the redundant CdSe quantum dots were washed away repeatedly with toluene, so that the purified first complex could be obtained.
Embodiment 2
[0033] Example 2: Preparation of the second complex
[0034] In order to carry out the silanization modification of the first complex, the precipitation of the first complex under ultrasonic conditions
[0035] Add 125 μL of n-octyltrimethoxysilane, followed by 9 mL of methanol and 112 μL of aqueous ammonia (25%). After mixing and sonicating for 30 min, centrifuge at 8000 rpm, and wash off excess n-octyltrimethoxysilane with methanol to obtain the purified first complex.
Embodiment 3
[0036] Embodiment 3: the preparation of the 3rd compound
[0037] For further epoxidation modification, first add the above silanized second complex to 1 μM sodium silicate solution and stir for reaction for more than 12 hours, then take 10 mL of the above solution and add 40 mL ethanol, 1.2 mL ammonia water and 50 μl orthosilicate tetra Ethyl ester was stirred and reacted for 20 min, and finally 50 μL of γ-(2,3-glycidoxy)propyltrimethoxysilane was added and stirred for 10 h, and washed twice by centrifugation with ethanol to obtain the purified third complex. Store in a 4°C refrigerator for later use.
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