A flexible microcolumn ring array for detecting clot contractility and its preparation method and application

A contraction force and microcolumn technology, applied in the field of biomedicine, can solve the problems of difficult clinical transformation, complicated device processing, and complicated operation, and achieve the effects of less sample consumption, simple processing, and simple and fast experimental operation

Active Publication Date: 2022-07-05
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the above-mentioned methods are complicated to process and require extremely sophisticated inspection platforms and complex data acquisition equipment, such as fluorescence microscopes and atomic force microscopes; the high cost of inspection makes it only suitable for mechanistic research, and it is difficult for clinical transformation
In addition, the above method needs to separate platelets from blood, which complicates the operation

Method used

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  • A flexible microcolumn ring array for detecting clot contractility and its preparation method and application
  • A flexible microcolumn ring array for detecting clot contractility and its preparation method and application
  • A flexible microcolumn ring array for detecting clot contractility and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0072] Embodiment 1 A flexible micropillar ring array and its chip for detecting clot contractility

[0073] like image 3 shown, a flexible micropillar ring array for detecting clot contractility, wherein the micropillar ring array consists of several micropillar rows arranged periodically, each micropillar row having several micropillar rings , each micro-column ring consists of four annular micro-columns; the micro-column ring has a circular hole with a certain diameter, when the clot contraction force is detected, whole blood is dropped into the circular hole of the micro-column ring Blood coagulation test was performed. The diameter of the micro-column ring is 1 mm, and the spacing of each micro-column ring is 1 mm; the column height of the micro-column is 1.2 mm, and the diameter of the upper surface of the column is 0.2 mm. The micropillar ring array is a circular array; the micropillars are cylindrical.

[0074] In addition, if image 3 As shown in (b) in the figur...

Embodiment 2

[0075] Example 2 A flexible micropillar ring array and its chip for detecting clot contractility

[0076] like image 3 shown, a flexible micropillar ring array for detecting clot contractility, wherein the micropillar ring array consists of several micropillar rows arranged periodically, each micropillar row having several micropillar rings , each micro-column ring consists of four annular micro-columns; the micro-column ring has a circular hole with a certain diameter, when the clot contraction force is detected, whole blood is dropped into the circular hole of the micro-column ring Blood coagulation test was performed. The diameter of the micro-column ring is 3 mm, and the spacing of each micro-column ring is 5 mm; the column height of the micro-column is 1.6 mm, and the diameter of the upper surface of the column is 0.4 mm. The micro-column ring array is a circular array, an elliptical array or a polygonal array; the micro-columns are cylindrical, elliptical or polygonal...

Embodiment 3

[0079] Embodiment 3 A kind of flexible micro-pillar ring array for detecting clot contractility and preparation method of its chip

[0080] A method for preparing the flexible micropillar ring array chip for detecting clot contractility, comprising the following steps:

[0081] S1. Use Comsol simulation software to simulate the scene of micro-column bending under force, process and design the micro-column, determine the feasible size and actual spring coefficient of the micro-column, and the relevant parameters of the PDMS material;

[0082] S2. According to the design result of S1, a mold is obtained by cutting an acrylic plate with a laser, and finally PDMS is used for reverse molding to produce the flexible micro-pillar ring array chip for detecting clot contraction force.

[0083] In the invention, the method of laser engraving and flexible material PDMS moulding is used to process the micro-pillar ring array or the micro-pillar ring array chip. Specifically:

[0084] 1....

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Abstract

The invention discloses a flexible micro-column ring array for detecting clot contractility and a preparation method and application thereof. The micro-column ring array is composed of several micro-column rows arranged periodically, each of the micro-column rows has several micro-column rings, and each of the micro-column rings is surrounded by four or more than four rings. The micro-column ring is composed of circular pores with a certain diameter, and when the clot contractility is detected, whole blood is dropped into the circular pores of the micro-column ring for coagulation test. The invention utilizes the interfacial tension of the solid-liquid-gas system and the resistance of the micro-column to efficiently fix the whole blood sample in the micro-column ring; meanwhile, the micro-column is used as a force sensor to measure the size of the clot contraction force in real time, the detection result is highly sensitive, and the detection It has low cost and easy clinical transformation; in addition, there is no need to separate platelets from blood, and whole blood can be used for detection, fully considering the influence of other components in blood on platelet contraction, and the results are more objective and comprehensive.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. In particular, it relates to a flexible micro-column ring array for detecting clot contractility and its preparation method and application. Background technique [0002] Coagulation is a very important physiological process that must be properly regulated by the human body to maintain a delicate balance between bleeding and thrombosis. Insufficient coagulation function can lead to excessive blood loss during trauma, and excessive coagulation function is often accompanied by the risk of developing blood clots. Coagulation function test plays an important role in clinical practice. During cardiopulmonary bypass, surgeons need to detect blood coagulation function immediately to guide the administration of procoagulants and anticoagulants to prevent blood from coagulating during cardiopulmonary bypass [1]. In addition, blood loss caused by surgery, contact coagulation activation, blood diluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49B23K26/38B23K26/402
CPCG01N33/49G01N33/4905B23K26/38B23K26/402
Inventor 李自达黄兰蛛郑卫东
Owner SHENZHEN UNIV
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