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Method for detecting HIV-1 drug resistance of pregnant and lying-in women

An HIV-1 and detection method technology, applied in the field of HIV-1 drug resistance detection in pregnant and lying-in women, can solve the problems of insufficient data, low drug resistance detection sensitivity, insufficient technical detection, etc., to improve sensitivity and ensure sustainable development. sex, guarantee scientific effect

Inactive Publication Date: 2020-08-21
昆明市官渡区妇幼保健计划生育服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (2) There are deficiencies in clinical research
[0008] The clinical application of ART in my country is late, the accumulated data is insufficient, and there are still some differences between generic drugs and original drugs, which may lead to new adverse drug reactions
[0009] (3) Insufficient detection of existing technologies
[0010] In the existing technology, the sensitivity of drug resistance detection is low when only plasma RNA is used for monitoring

Method used

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  • Method for detecting HIV-1 drug resistance of pregnant and lying-in women
  • Method for detecting HIV-1 drug resistance of pregnant and lying-in women
  • Method for detecting HIV-1 drug resistance of pregnant and lying-in women

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Experimental program
Comparison scheme
Effect test

Embodiment

[0044] 1. Experiment preparation

[0045] 1. Experimental reagents and equipment

[0046]Biological safety cabinet (NV-S430-400E, NVAIR, USA), PCR amplification instrument (ABI 9700, ABI Biotechnology Company, USA), high-speed centrifuge (5415C, eppendorf, Germany), FACSCalibur flow cytometer (BD Company, USA), Viral load meter (b DNA) (Versant molecule 440, Siemens, Germany), HIV-1 viral load detection sample preparation and analysis system (M2000sp Real Time, Abbott, USA), ultra-low temperature refrigerator (-86°C) (FORMA, USA), electrophoresis Instrument (Beijing Liuyi Instrument Factory), BD Falcon flow cytometry specimen detection tube (BD Company, USA), 10ul, 20ul, 100ul, 200ul, 1000ul pipette (Eppendorf Company, Germany).

[0047] 2. Main reagents

[0048] Viral load reagent, Versant HIV-1RNA 3.0 Assay (b DNA), Siemens, Germany; HIV-1 nucleic acid extraction reagent, Sample preparation System RNA, Abbott, USA; PCR amplification reagent, Ta Ka Ra OneStepRNA PCR Kit and...

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Abstract

The invention discloses a method for detecting HIV-1 drug resistance of pregnant and lying-in women, and relates to the technical field of biology. The method comprises the following steps: collectingand separating blood of a pregnant and lying-in woman HIV patient; counting the CD4+T lymphocytes and detecting the virus load; extract RNAan from specimens with the virus number larger than 1000 copies / ml; taking the RNA in the previous step as a template, carrying out primary.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a detection method for HIV-1 drug resistance of pregnant women. Background technique [0002] AIDS (acquiredimmuneficiency syndrome, AIDS) is a human immunodeficiency virus (humanimmunodeficiency virus, HIV) infection caused by a very harmful infectious disease. However, with the continuous development and improvement of antiretroviral therapy (ART), ART can achieve virus suppression and immune reconstruction, thereby prolonging the survival period and improving the quality of life of HIV / AIDS patients. However, with the development of large-scale antiviral treatment, drug resistance and viral rebound under poor treatment conditions may lead to the failure of antiviral treatment. serious problem. [0003] Therefore, the importance of drug resistance surveillance goes deep into every country. In developed countries, the detection of antiviral treatment failure and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6806C12R1/93
CPCC12Q1/703C12Q1/6806C12Q2600/156C12Q2600/106
Inventor 冯磊柏灿红吴爱辉
Owner 昆明市官渡区妇幼保健计划生育服务中心