Pre-activated hydrophilic magnetic microsphere and preparation method thereof

A magnetic microsphere, pre-activation technology, applied in the field of materials, can solve the problems of difficult to control batch difference, difficult to long-term storage, harsh storage conditions, etc., and achieve the effects of low cost, strong hydrophilicity, and easy availability of raw materials

Active Publication Date: 2020-08-25
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these magnetic microspheres have certain deficiencies and defects. The magnetic microspheres modified by functional groups such as carboxyl groups and amino groups need to add activating substances. The operation is slightly cumbersome, and it is difficult to control the difference between batches. Improper activation steps such

Method used

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  • Pre-activated hydrophilic magnetic microsphere and preparation method thereof
  • Pre-activated hydrophilic magnetic microsphere and preparation method thereof
  • Pre-activated hydrophilic magnetic microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Prepare a mechanical stirrer and a water bath, install a 100ml three-neck flask, add 20ml of N,N-dimethylformamide, add 10g of polyethylene glycol (molecular weight: 800) while stirring, and stir for about 30 minutes to complete Dissolved, weighed 0.8g of toluenesulfonyl chloride and added to the reaction solution, and at the same time, added dropwise 0.2ml of triethylamine to the reaction solution, and reacted for 6 hours.

[0039] (2) After the reaction is over, prepare several ultrafiltration tubes, dilute the reaction solution with 40ml purified water, put the diluted reaction solution into the ultrafiltration tube, select an ultrafiltration tube with a molecular weight cut-off of 300, and place it in a centrifuge for centrifugation. The machine is set at 4000 rpm. During the ultrafiltration process, a conductivity meter is required for monitoring, and the ultrafiltration is completed until the conductivity is <10us / cm.

[0040] (3) After the dialysis, all the l...

Embodiment 2

[0044] (1) Prepare a mechanical stirrer and a water bath, install a 100ml three-neck flask, add 30ml N,N-dimethylformamide, add 6g polyhydroxyethyl methacrylate (molecular weight: 2500) while stirring, and stir Dissolve completely in about 30 minutes, weigh 0.3 g of toluenesulfonyl chloride and add to the reaction solution, and at the same time add 0.1 ml of triethylamine dropwise to the reaction solution, and react for 6 hours.

[0045] (2) After the reaction is over, prepare some ultrafiltration tubes, dilute the reaction solution with 80ml of purified water, put the reaction solution in the ultrafiltration tube, select the ultrafiltration tube with a molecular weight cut-off of 500, and place it in a centrifuge for centrifugation. The centrifuge is set to 4000 revolutions per minute, a conductivity meter is required to monitor the ultrafiltration process, and the ultrafiltration is completed until the conductivity is <10us / cm.

[0046] (3) After the dialysis, all the liquid...

Embodiment 3

[0050] (1) Prepare a mechanical stirrer and a water bath, install a 100ml three-neck flask, add 70ml of N,N-dimethylformamide, add 7g of polyethylene glycol (molecular weight: 3000) while stirring, and stir for about 30min to complete Dissolved, weighed 0.35g of toluenesulfonyl chloride and added to the reaction solution, and at the same time, added dropwise 0.2ml of pyridine to the reaction solution, and reacted for 6 hours.

[0051] (2) After the reaction is over, prepare some ultrafiltration tubes, dilute the reaction solution with 70ml purified water, put the reaction solution in the ultrafiltration tube, select the ultrafiltration tube with a molecular weight cut-off of 800, and place it in a centrifuge for centrifugation. The centrifuge is set to 4000 revolutions per minute, a conductivity meter is required to monitor the ultrafiltration process, and the ultrafiltration is completed until the conductivity is <10us / cm.

[0052] (3) After the dialysis, all the liquid in th...

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Abstract

The invention provides a preparation method of a pre-activated hydrophilic magnetic microsphere. Toluenesulfonyl groups are contained on the surfaces of the magnetic microspheres, EDC or glutaraldehyde activation treatment is not needed, the magnetic microspheres can be directly covalently coupled and combined with amino-containing biomolecules through the toluenesulfonyl groups, and the preparation method comprises the following steps: carrying out toluenesulfonylation modification on a hydroxyl-containing hydrophilic polymer; mixing and reacting the tosylated hydrophilic polymer with amino magnetic beads to obtain the pre-activated tosyl magnetic microspheres. The magnetic microsphere has the characteristic of pre-activation; toluenesulfonyl on the surface of the pre-activated magnetic microsphere is coupled with the magnetic microsphere through amino, the length of a spacer arm of the pre-activated magnetic microsphere is longer than that of a space arm of a common functional groupmodified magnetic microsphere, meanwhile, due to the existence of a hydrophilic polymer structure, the surface of the magnetic microsphere is hydrophilic, and lower non-specific adsorption is achievedin the application process. The method has the advantages of simple and easily available raw materials, low cost, simple process steps and very strong practicability.

Description

technical field [0001] The invention relates to a method for preparing surface preactivated magnetic microspheres in the field of materials, in particular to a method for preparing preactivated hydrophilic magnetic microspheres. Background technique [0002] In recent years, with the rapid development of nucleic acid diagnosis and chemiluminescence immunoassay diagnosis, as one of the important raw materials of the technology platform, magnetic microspheres have been widely used. It is a new type of functional solid carrier, which has solid carrier and magnetic material It can be moved and concentrated under the action of an external magnetic field. When the external magnetic field is removed, it can be evenly dispersed in the liquid with a little vibration or suction, so that the separation of solid and liquid phases becomes very fast and convenient. Through simple High-purity target substances can be obtained by elution; the surface of magnetic microspheres is modified wit...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30
CPCB01J20/103B01J20/265B01J20/24B01J20/28009B01J20/28021
Inventor 周国庆江晓红
Owner NANJING UNIV OF SCI & TECH
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