Method for researching proteome change of rat suffering from depression or anxiety based on proteomics

Abstract

The invention discloses a method for researching proteome change of a rat suffering from depression or anxiety based on proteomics. The method comprises the following steps: 1, carrying out rat brainforehead cortex separation and protein extraction; 2, carrying out FASP method enzymolysis, polypeptide iTRAQ labeling, SCX grading and LC-MS/MS mass spectrometry analysis on the protein sample obtained in the step 1; 3, performing library search and bioinformatics analysis on the mass spectrum data obtained in the step 2; and 4, carrying out PRM technical verification on the differential proteinobtained in the step 3. According to the invention, 1, the preclinical model constructed by using the CMS can help to expose potential molecular characteristics of depression and anxiety disorder; 2,depression and anxiety cause reduction of prefrontal cortex cerebral volume and dendritic spine of human beings or animals; and 3, an iTRAQ quantitative proteomics method is adopted to carry out difference analysis on the expression levels of the forehead leaf proteins of the four groups of depression/anxiety rat models, so that the reliability, objectivity and accuracy of a quantitative proteomics result are improved.

Description

technical field [0001] The invention relates to a method for proteomics-based research on proteome changes in depression or anxiety rats, belonging to the technical field of biomedicine. Background technique [0002] Depression and anxiety are two common chronic neurological diseases that have a negative impact on the patient's social life, relatives, family and society. Many researchers have found that depression and anxiety share the same risk factors, including chronic stimulation and life stress. Much evidence shows that chronic stressful living is an environmental risk factor for depression and anxiety disorders. Despite exposure to chronic stress, many individuals do not display symptoms of anxiety or depression. Chronic stress (CMS) has been widely used to induce depression and anxiety behaviors in rats, establishing a model of environmental factors affecting humans. To uncover the underlying biological etiology and pathophysiology of depression and anxiety disorde...

AI Technical Summary

Problems solved by technology

Prefrontal plasticity is abnormally found in both depressed and anxious individuals, and corresponding internal patterns may be fundamentally altered and thus far remain poorly understood

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