Application of Space-Mutated Saccharomyces cerevisiae st26-22 in Brewing Beer

A technology of Saccharomyces cerevisiae and beer, applied in the biological field, can solve the problems affecting the economic benefits of beer enterprises, slow diacetyl reduction, long fermentation cycle, etc., and achieve the effects of prolonged beer clarification time, dense precipitation, and low diacetyl content

Active Publication Date: 2022-03-08
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country uses the following fermenting yeast, that is, grape juice yeast (S.uvaram) to brew beer. Its traditional fermentation cycle is long, post-fermentation diacetyl reduction is slow, and the wine age is long, which affects the economic benefits of beer companies.

Method used

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  • Application of Space-Mutated Saccharomyces cerevisiae st26-22 in Brewing Beer
  • Application of Space-Mutated Saccharomyces cerevisiae st26-22 in Brewing Beer
  • Application of Space-Mutated Saccharomyces cerevisiae st26-22 in Brewing Beer

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1. Screening of Excellent strains with space mutagenesis brew yeast fermentation performance

[0057] 1, strain

[0058] Space Mutagenevisiae ST26: Safaromyces Cerevisiae GT26 Tiang Tiangong No.2 and Shenzhou-11 spacecraft equipped with returned space induced strains, Guarana Bioengineering Technology (Beijing) Limited Space Microbial Strokes. In order to distinguish the difference in strains before and after the aerospace, Saccharomyces Cerevisiae GT26 space mounted as a space mutagenesis Saccharomyces Cerevisiae ST26.

[0059] 2, spatial mutagenesis of wine yeast isolation and purification

[0060] Ground brewing yeast GT26 glycerol and space in -80 ° C will be frozen in 4 ° C, in the sterile workbench, inoculated to 10 ml of inoculation in the sterile workbench. In the mild sprout culture medium, at 28 ° C for 18 hours, after activation of the three generations, 1 ml of the gas-activated medium after the activation of the GT26 and ST26 was used to move into a ste...

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PUM

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Abstract

The invention discloses the application of space mutagenesis Saccharomyces cerevisiae ST26-22 in brewing beer. The space mutagenized Saccharomyces cerevisiae ST26-22 disclosed in the present invention has the preservation number of CGMCC No.19246 in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee. In the present invention, high-quality wine-brewing saccharomyces cerevisiae is subjected to space mutagenesis, and a yeast with excellent fermentation performance suitable for brewing beer is screened, that is, space-mutated Saccharomyces cerevisiae ST26-22, which is not only suitable for low-temperature fermentation, but also The ability to produce diacetyl is low, which is of great significance for reducing diacetyl content, improving wine body quality, shortening wine storage time, and improving the production efficiency of beer enterprises.

Description

Technical field [0001] The present invention relates to the application of spatial mutagenesis in the field of biotechnology in the field of brewing beer. Background technique [0002] Saccharomyces Cerevisiae is a single-cell eukaryotic microorganism that is mainly budding, microscopic bacteria cells are elliptical or oval, monochrome, one end germination, and anaerobic bacteria. The colonies on the YEPD medium plate are circular, the size is 4 ~ 6mm, the surface projection, the smooth moist, the edge is neat, the texture is viscous, opaque, the color is milky, there is a typical wine flavor. Bacillus can perform aerobic breathing under aerobic conditions, produce carbon dioxide and water through the EMP pathway and release energy, grow metabolism, and prosperity. Under an oxygen-free conditions, fermentation can be carried out and alcohol is generated. The brewing yeast produced by the fermentation performance, not only has the reproductive speed, which can withstand higher sug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12C11/02C12C12/00C12R1/865
CPCC12C11/003C12C11/02C12C12/006
Inventor 郝红炜刘慧袁明张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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