Method of cultivating algae and photobioreactor

A technology of algae cells and cultures, applied in the field of cultivating algae, which can solve problems such as too much light and unhealthy growth

Pending Publication Date: 2020-09-04
NICHIA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Cultivation in an open system is suitable for large-scale production, but if the concentration of the microalgae initially introduced at the beginning of the culture is not high enough, there is a problem that too much light is irradiated per cell, and healthy growth cannot be achieved. Case

Method used

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  • Method of cultivating algae and photobioreactor
  • Method of cultivating algae and photobioreactor
  • Method of cultivating algae and photobioreactor

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preparation example Construction

[0157]

[0158] From another point of view, the present invention provides a method for preparing a cell culture of algae selected from the group consisting of green algae, euglena, diatoms, and dinoflagellates.

[0159] The method for producing a culture of algae cells of the present invention (hereinafter also referred to as "the production method of the present invention") is characterized in that the culture selected from green algae , euglena, diatoms, and dinoflagellates algae.

[0160] The algal cells used in the production method of the present invention are cells of algae selected from the group consisting of green algae, euglena, diatoms, and dinoflagellates as described in the above .

[0161] According to the production method of the present invention, it is possible (for example, from the viewpoint of time and / or cost) to produce a culture of algae cells efficiently and / or at a high density (that is, high biomass productivity can be achieved), Further, useful s...

Embodiment

[0385] Experiment 1: Determination of Absorption Spectrum of Chlamydomonas

[0386] method:

[0387] Chlamydomonas reinhardtii CC-125 strain was pre-cultured for 3 days using a bottle-type photobioreactor under the following conditions.

[0388] pre-culture conditions

[0389] Light: white fluorescent lamp (optical quantum flux density: 200μmol / m 2 / s)

[0390] Medium: Change the amount of nitrogen source to 5 times (add NH 4 Cl to 2.5g / L) HSM medium 500mL

[0391] CO 2 Concentration: supply 2% CO to the medium at 0.1mL / mL medium / min 2 (v / v)

[0392] Temperature: 30°C

[0393] Stirring: 500rpm

[0394] After pre-cultivation, dilute to make the optical density (OD 750 )=0.4 (equivalent to 0.15 g / L in terms of dry weight), the main culture was carried out.

[0395] The main culture was carried out for 2 days under the following conditions.

[0396] Main culture condition (OD at the beginning 750 =0.4)

[0397] Light: white fluorescent lamp (optical quantum flux densi...

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Abstract

A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae includes: irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and measuring a condition of the algal cellsand/or a condition of an algal cell culture provided by cultivating the algal cells. Irradiation and non-irradiation of the algal cells with the artificial light are switched, or the photon flux density in the wavelength range of 520-630 nm is changed, according to the measured condition of the algal cells and/or the measured condition of the algal cell culture.

Description

technical field [0001] The present invention relates to a method for cultivating algae and a photobioreactor for such culturing. Background technique [0002] Microalgae (eg, Haematococcus pluvialis, Chlorella, and Euglena) are actively cultivated for the production of raw materials for health foods or supplements, or oils suitable for biofuels. [0003] Microalgae are cultured in a closed system using artificial light sources (eg, in a closed type photobioreactor) or in an open system using sunlight (eg, an open type in an open pond). [0004] Cultivation in an open system is suitable for large-scale production, but if the concentration of the microalgae initially introduced at the beginning of the culture is not high enough, there is a problem that too much light is irradiated per cell, and healthy growth cannot be achieved. Case. Therefore, it is necessary to prepare microalgae in a somewhat high concentration in advance, and for this preparation, cultivation in a close...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12M1/36C12M1/00C12R1/89
CPCC12N1/12C12M21/02C12M23/02C12M41/06C12M41/34A01G33/00C12N13/00C12M41/00C12M41/10C12M31/00Y02A40/80
Inventor 莲沼诚久加藤悠一藤川康夫鹤本智大
Owner NICHIA CORP
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