Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of lightening type fluorescent compound in preparation of liver cancer diagnosis kit and dyeing method

A technology of fluorescent compounds and diagnostic kits, applied in chemical instruments and methods, fluorescence/phosphorescence, preparation of samples for testing, etc. Effect

Pending Publication Date: 2020-09-04
HUBEI UNIV OF EDUCATION +2
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] An object of the present invention is to provide an application of a light-emitting fluorescent compound in the preparation of a liver cancer diagnostic kit to solve the technical problem of low diagnostic efficiency in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of lightening type fluorescent compound in preparation of liver cancer diagnosis kit and dyeing method
  • Application of lightening type fluorescent compound in preparation of liver cancer diagnosis kit and dyeing method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] The preparation method of ZZ-HPB-NC may comprise the following steps:

[0054] (1) Add 1.00mmol 4-formylphenylboronic acid, 1.00mmol toluene, 0.025mmol palladium acetate, 1.00mmol silver carbonate, and 3mL mixed solvent of n-propanol and water into a 25mL three-necked flask, blow nitrogen into it, and Under stirring, react at 120°C for 60 min, filter with suction and spin the filtrate to dry to obtain the crude product I; dissolve the crude product I in 3 mL of dichloromethane, use petroleum ether and dichloromethane as eluents, and use a column Chromatographic separation and purification gave a light yellow solid;

[0055] (2) Add 0.2mmol of the light yellow solid obtained in step (1) and 0.48mmol of methyl 2-cyanoacetate into a 100mL three-necked flask, then add 15mL of methanol and 0.03mL of piperidine, and react at 60°C under stirring After 3h, the solid precipitated out after the reaction, and the reaction crude product II was obtained; the reaction crude product ...

Embodiment 1

[0057] This example provides an application of a light-up fluorescent compound in the preparation of a liver cancer diagnostic kit. The structural formula of the light-up fluorescent compound is as follows:

[0058] Abbreviated as ZZ-HPB-NC.

[0059] Specifically include the following steps:

[0060] (1) Dissolve ZZ-HPB-NC in DMSO to make the concentration of ZZ-HPB-NC 1×10 -2 mol / L solution a;

[0061] (2) Dilute the solution a obtained in step (1) with high-sugar DMEM medium, and prepare the concentration of ZZ-HPB-NC as 1×10 -5 mol / L solution b to obtain a fluorescent probe, which can be used as a kit.

Embodiment 2

[0063] This example provides an application of a light-up fluorescent compound in the preparation of a liver cancer diagnostic kit. The structural formula of the light-up fluorescent compound is as follows:

[0064] Abbreviated as ZZ-HPB-NC.

[0065] Specifically include the following steps:

[0066] (1) Dissolve ZZ-HPB-NC in DMSO to make the concentration of ZZ-HPB-NC 1×10 -4 mol / L solution a;

[0067] (2) Dilute the solution a obtained in step (1) with physiological saline, and prepare the concentration of ZZ-HPB-NC as 1×10 -8 mol / L solution b to obtain a fluorescent probe, which can be used as a kit.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biosensing and imaging, in particular to an application of a lightening type fluorescent compound in preparation of a liver cancer diagnosis kit and a dyeing method. According to the application of the lightening type fluorescent compound in preparation of the liver cancer diagnosis kit, the structural formula of the lightening type fluorescent compound is shown in the specification, wherein R1 and R2 are independently selected from alkyl. According to the present invention, with the application of the lightening type fluorescent compound, the cancer cells and the normal cells can be rapidly and accurately discriminated and positioned on the premise of no external targeting group modification, and the toxic-side effect is small, the time consumption is short and the cost is low; and a new clinical strategy is provided for intraoperative lesion property rapid diagnosis of liver tumor resection, liver cancer resection surgical incision identification and fluorescence surgical navigation.

Description

technical field [0001] The invention relates to the technical field of biosensing and imaging, in particular to the application and dyeing method of a light-up fluorescent compound in the preparation of a liver cancer diagnostic kit. Background technique [0002] Hepatocellular carcinoma (hereinafter referred to as liver cancer) is the most common malignant liver tumor, which originates from liver epithelial cells. In recent years, its incidence rate has increased and its mortality rate remains high. Surgical resection is currently the most effective treatment for liver cancer. Imaging examinations such as enhanced CT, enhanced MRI, or contrast-enhanced ultrasound combined with tumor markers are often used to make a clinical diagnosis before surgery. However, some patients do not have typical imaging features. And 20 to 30% of patients with tumor markers do not increase. However, the benign and malignant nature of the lesion determines the surgical method and the extent of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/30G01N21/64C09K11/06
CPCG01N1/30G01N21/6428G01N21/6486C09K11/06G01N2021/6439C09K2211/1007
Inventor 陈笛笛李民毛慧灵董宇平
Owner HUBEI UNIV OF EDUCATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com