Multi-channel synchronous fluorescence immunochromatography detection system

Abstract

The invention belongs to the technical field of immunochromatography detection, and particularly relates to a multichannel synchronous fluorescence immunochromatography detection system. The system comprises a bottom plate, a multi-channel fluorescence immunochromatography reagent card and a multi-channel fluorescence immunochromatography detection device; the bottom plate completes positioning ofthe multichannel fluorescence immunochromatography reagent card and installation and fixation of the multichannel fluorescence immunochromatography detection device; and the multichannel fluorescenceimmunochromatography detection device completes detection, imaging and reading of the multichannel fluorescence immunochromatography reagent card. The system has the parallel detection capability of20 channels, and the synchronous detection of 20 targets can be realized by single sample adding; the detection efficiency of unknown pathogenic microorganisms is greatly improved; and the operation difficulty of inspectors under the field condition is greatly reduced, and the operation time in the potential risk environment is shortened.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography detection, in particular to a multi-channel synchronous fluorescence immunochromatography detection system. Background technique [0002] Fluorescence immunochromatography technology is a new quantitative detection technology combining immunofluorescence technology and traditional immunochromatography technology. In this technology, the strip-shaped fiber chromatography material immobilized with detection lines (coated antibodies or coated antigens) and quality control lines (anti-antibodies) is used as the stationary phase, the test solution is used as the mobile phase, and fluorescently labeled antibodies or antigens are fixed on the connection pads. , to move the analyte across the strip by capillary action. For macromolecular antigens (proteins, viruses, pathogenic bacteria, etc.) with multiple antigenic determinants, a "sandwich" double-antibody sandwich immunochromatography met...

AI Technical Summary

Problems solved by technology

[0003] However, the current fluorescent immunochromatography detection technology is mainly based on single-target detection, that is, one test strip and one sample can only detect one item. Although there are reports that multi-target detection is proposed, it can only detect three targets at the same time. However, the detection of food-borne pathogens and pathogenic microorganisms in environmental samples and the preliminary screening of respiratory pathogens under unknown conditions are far from meeting the requirements. There are dozens of food-borne pathogens, and the main ones are More than a dozen kinds; there are also dozens of common respiratory pathogenic bacteria. Conventional fluorescence immunochromatography detection methods for sampling require multiple sampling and detection, and repeated operations are required, which is not conducive to faster work efficiency and elimination. The detection error between each sample detection

Images