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Kit and method for detecting quality of enzyme immunoassay microwell plate

A microplate and kit technology, which is applied in the field of kits for detecting the quality of enzyme-free microplates, can solve the problems of lack of versatility and low inspection efficiency, and achieve wide versatility, comprehensive inspection, and saving manpower and material resources. Effect

Active Publication Date: 2020-09-04
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has low inspection efficiency, is not universal, and only evaluates an internal control with a signal value of about 2.0

Method used

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  • Kit and method for detecting quality of enzyme immunoassay microwell plate
  • Kit and method for detecting quality of enzyme immunoassay microwell plate
  • Kit and method for detecting quality of enzyme immunoassay microwell plate

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Embodiment 1

[0063] The method for checking the enzyme-free microplate of the present invention comprises the following specific steps:

[0064] The first step, appearance inspection: observe the product to be inspected with the naked eye, the texture is uniform, no stains, the hole bar is clear and transparent, and there is no incomplete breakage and handle phenomenon; the wall thickness of the hole bar cup is uniform, the color is uniform, the bottom of the hole is smooth, and there is no impurity;

[0065] The second step is the matching test: the hole bar and the plate frame are easy to install, and after installation, they are tight and not loose; there is no warping phenomenon;

[0066] The third step, blank value: read the ELISA microplate directly on the microplate reader with a wavelength of 450nm, and the signal value of each plate should be less than or equal to 0.05;

[0067] The fourth step is to prepare CB buffer solution, Casein enzyme dilution solution and X solution, and p...

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Abstract

The invention relates to the field of in-vitro diagnosis, in particular to a kit and a method for detecting the quality of an enzyme immunoassay microwell plate. The kit comprises coating liquid, confining liquid, a low-value quality control material Q1, a high-value quality control material Q2, substrate liquid and a color developing agent. The formula of the confining liquid is 0.5-0.7 g of NaH2PO4, 5-7 g of Na2HPO4, 8-10 g of NaCl, 8-12 g of Casein, 1-3 g of KCl, 45-55 g of sucrose, 0.8-1.2 g of ADP, 0.8-1.2 mL of Tween-20, 0.8-1.2 mL of a preservative and the balance of water. The detection method is simple, easy to operate and wide in universality, independent detection on each kit is not needed, and manpower and material resources are greatly saved; the method is comprehensive in inspection, and the inspection result is real and reliable; and the quality of the enzyme immunoassay microwell plate can be strictly ensured.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a kit and a method for detecting the quality of an enzyme-immune microplate. Background technique [0002] The principle of enzyme-linked immunoassay: ① Make antigen or antibody bind to the surface of a certain solid phase carrier and maintain its immunological activity. ②The antigen or antibody is linked with an enzyme to form an enzyme-labeled antigen or antibody, which not only retains its immune activity, but also retains the activity of the enzyme. During the determination, the test specimen (determining the antibody or antigen in it) and the enzyme-labeled antigen or antibody react with the antigen or antibody on the surface of the solid phase carrier according to different steps. The antigen-antibody complex formed on the solid-phase carrier is separated from other substances by washing, and the amount of enzyme bound to the solid-phase carrier is proportional to the amo...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/58
CPCG01N33/543G01N33/581Y02A50/30
Inventor 张金蕊邢政刘利晶葛建臣陈静李彬
Owner AUTOBIO DIAGNOSTICS CO LTD
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