Kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of small cell lung cancer patient

Abstract

The invention provides a kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of a small cell lung cancer patient, and belongs to the technical field of molecular biology. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a mouse anti-human E-Cadherin monoclonal antibody, goat anti-mouse IgG / HRP, SABC, 0.1% Triton X-100, 0.3% H2O2, a reagent A, a reagent B and a 6 * PBS buffer solution. According to the detection method provided by the invention, the E-Cadherin expression condition of the patient suffering from advanced or recurrent small cell lung cancer can be detected without obtaining a tissue specimen through needle biopsy. The technology belongs to a minimally invasive technology, and real-time detection can be realized; the false positive result caused by the edge effect possibly generated in the dyeing process can be avoided, the stability is high, the cell loss is reduced, and the detection accuracy is improved.

Description

technical field [0001] The invention provides a kit and a method for detecting E-Cadherin gene mutation in peripheral blood circulating tumor cells of patients with small cell lung cancer, and belongs to the technical field of molecular biology. Background technique [0002] Lung cancer is one of the main malignant tumors leading to the death of cancer patients. In my country, the morbidity and mortality of lung cancer rank first. Small cell lung cancer (SCLC) accounts for about 15% to 20% of the incidence of lung cancer. Compared with non-small cell lung cancer, it has the characteristics of faster tumor doubling time, rapid growth and early metastasis. [0003] Circulating tumor cells (CTCs) are tumor cells shed from solid tumors and enter the peripheral blood circulation. Since their discovery in 1989, a variety of methods have been used to detect circulating tumor cells in peripheral blood. Recent studies have shown that its detection has important clinical significance...

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the inability of patients with advanced or recurrent small cell lung cancer to obtain tissue samples by real-time or repeated puncture, and thus to evaluate the status of E-Cadherin, the present invention provides a non-diagnostic detection method for ALK gene mutations in peripheral blood circulating tumor cells of patients with small cell lung cancer. Detection method: CTCs in the peripheral blood of patients with advanced or recurrent small cell lung cancer who cannot obtain tissue samples are separated by membrane filtration devices, and the expression of E-Cadherin in CTCs is further detected by immunohistochemical technology

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