Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and application of aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate

A technology of ore leaching and field application in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as intolerance and insufficient denitrification ability, and achieve good tolerance and adaptability

Active Publication Date: 2020-09-11
WUHAN INSTITUTE OF TECHNOLOGY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies such as the intolerance of foreign denitrification bacteria species to the leachate from rare earth leaching sites and the insufficient denitrification ability in the existing microbial denitrification technology, and develop the leachate from rare earth leaching sites Indigenous high-efficiency denitrification strains Acinetobacter beinii A9 and Citrobacter youngae A13 provide a new option for industrial application of high-efficiency, environment-friendly and low-cost microbial denitrification of leachate from rare earth leaching sites

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and application of aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate
  • Aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and application of aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate
  • Aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and application of aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Inoculate the indigenous high-efficiency denitrification bacterial strain A9 from the rare earth leaching site leachate stored on the inclined surface of the test tube at 4°C into a plate containing a solid medium for denitrification (see 3-(2) for the preparation method of the plate), and inoculate at 28°C Inverted cultivation for 12 hours to realize the activation of the strain. Under sterile conditions, use an inoculation loop to inoculate the activated strain into a 250mL Erlenmeyer flask filled with 100mL of denitrification liquid medium, place the Erlenmeyer flask in a shaker and culture it for 12 hours at 28°C and 165r / min. , to obtain the seed solution. Based on the denitrification liquid medium, the initial concentration of ammonia nitrogen was adjusted to 100 mg / L, and the C / N ratio was 12 to obtain a simulated leachate from a rare earth leaching site. The seed solution was inoculated into 100mL of the sterilized simulated leachate from the rare earth leachin...

Embodiment 2

[0042] Seed liquid was prepared with reference to the method of Example 1. Based on the denitrification liquid medium, the initial concentration of ammonia nitrogen was adjusted to 200 mg / L, and the C / N ratio was 12 to obtain a simulated leachate from a rare earth leaching site. The seed solution was inoculated into 100mL of the sterilized simulated leachate from the rare earth leaching site with 2% inoculum amount, and the shaking culture was carried out under the conditions of 28°C and 165r / min, and samples were taken regularly to measure the ammonia nitrogen content, OD600, Nitrite nitrogen content, nitrate nitrogen content, drawn to get Figure 4 .

[0043] Depend on Figure 4 It can be seen that the concentration of ammonia nitrogen in the simulated leachate of the rare earth leaching site dropped from the initial 200mg / L to 1mg / L after 36 hours of inoculation seed solution treatment, and the removal rate of ammonia nitrogen was as high as 99.5%. At the same time, no ch...

Embodiment 3

[0045] Seed liquid was prepared with reference to the method of Example 1. Based on the denitrification liquid medium, the initial concentration of ammonia nitrogen was adjusted to 300 mg / L, and the C / N ratio was 12 to obtain a simulated leachate from a rare earth leaching site. The seed solution was inoculated into 100mL of the sterilized simulated leachate from the rare earth leaching site with 2% inoculum amount, and the shaking culture was carried out under the conditions of 28°C and 165r / min, and samples were taken regularly to measure the ammonia nitrogen content, OD600, Nitrite nitrogen content, nitrate nitrogen content, drawn to get Figure 5 .

[0046] Depend on Figure 5 It can be seen that the concentration of ammonia nitrogen in the simulated leachate of the rare earth leaching site dropped from the initial 300 mg / L to 5.79 mg / L after 48 hours of inoculation seed solution treatment, and the calculation shows that the removal rate of ammonia nitrogen is as high as...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and an application of theaboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate. The strains respectively belong to Acinetobacter baylyi and Citrobacter youngae, which are transferred to the China Center for Type Culture Collection for preservation on January 10th, 2020, and corresponding preservation numbers are CCTCC NO:M 2020031 and CCTCC NO:M 2020030. The two strains are both from the rare earth mineral leaching space leachate, are both heterotrophic nitrification-aerobic denitrification denitrification strains, can efficiently remove ammonia nitrogen in the rare earth mineral leaching space leachate through an organic carbon source under the aerobic condition, besides, also have better removing effects on nitrate andnitrite, and have the advantages of being high in adaptability, high in denitrification efficiency and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms and sewage treatment, in particular to the native high-efficiency denitrification bacterial strains Acinetobacter beinerii A9 and Citrobacter youngae A13 in leachate from a rare earth leaching site and applications thereof. Background technique [0002] Rare earth elements are more and more widely used in new materials, new technologies, new energy and many other fields, and are the national development strategic reserve resources of many countries. my country is rich in rare earth resources, with the advantages of complete formula of rare earth elements and large reserves. These rare earth resources have extremely high economic and strategic value for our country. Ionic rare earth ore is a unique rare earth ore in my country, which contains high medium and heavy rare earths. Since the first development and utilization of ionic rare earth ore in the 1970s, pool leaching, heap leaching and i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC12N1/20C02F3/34C02F2101/16C12R2001/01C12N1/205
Inventor 肖春桥胡锦刚池汝安
Owner WUHAN INSTITUTE OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com