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Tissue culture method for increasing regeneration rate of mature wheat embryos

A technique for tissue culture and mature embryos, which is applied in the field of plant tissue culture and can solve problems such as studies that have not yet been reported.

Inactive Publication Date: 2020-09-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the function of HDACs in plants mainly focuses on model plants such as Arabidopsis thaliana, and there is no report on the study of HDACs in tissue culture of mature wheat embryos

Method used

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  • Tissue culture method for increasing regeneration rate of mature wheat embryos
  • Tissue culture method for increasing regeneration rate of mature wheat embryos
  • Tissue culture method for increasing regeneration rate of mature wheat embryos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Acquisition of mature wheat seeds:

[0034] CB037, Fielder and China Spring were sown in the farm of Experimental Station of Shandong Agricultural University in March, and mature seeds were harvested in June of the same year.

[0035] 2. Sterilization of mature wheat seeds:

[0036] Select mature and plump, harvested, undamaged CB037, Fielder and Chinese spring seeds, pour 75% ethanol (v / v) into the ultra-clean workbench, shake the table at 180 rpm, shake and disinfect for 10 minutes, and then use 25 % sodium hypochlorite (sodium hypochlorite stock solution available chlorine ≥ 10%) (v / v) disinfection for 25 minutes, rinsed with sterile water 4 times, submerged in sterile water for seeds overnight in a 25°C incubator. The next morning, disinfect with 25% sodium hypochlorite (sodium hypochlorite stock solution available chlorine ≥ 10%) (v / v) for 15 minutes, and rinse with sterile water 4 times.

[0037] 3. Tissue culture of mature wheat embryos

[0038] (1) Pre-cul...

Embodiment 2

[0055] 1. Acquisition of mature wheat seeds

[0056] CB037, Fielder and China Spring were sown in the farm of Experimental Station of Shandong Agricultural University in March, and mature seeds were harvested in June of the same year.

[0057] 2. Sterilization of mature wheat seeds

[0058] Select mature and plump, harvested, undamaged CB037, Fielder and Chinese spring seeds, pour 75% ethanol (v / v) into the ultra-clean workbench, shake the table at 180 rpm, shake and disinfect for 10 minutes, and then use 25 % sodium hypochlorite (sodium hypochlorite stock solution available chlorine ≥ 10%) (v / v) disinfection for 25 minutes, rinsed with sterile water 4 times, submerged in sterile water for seeds overnight in a 25°C incubator. The next morning, disinfect with 25% sodium hypochlorite (sodium hypochlorite stock solution available chlorine ≥ 10%) (v / v) for 15 minutes, and rinse with sterile water 4 times.

[0059] 3. Tissue culture of mature wheat embryos

[0060] (1) Pre-culti...

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Abstract

The invention discloses a tissue culture method for increasing the regeneration rate of mature wheat embryos, and belongs to the technical field of plant tissue culture. In the tissue culture processof mature wheat embryos, a histone deacetylase inhibitor with a certain concentration is added, and the induction rate and regeneration rate of embryogenic calluses can be effectively increased. Especially, 200 mu m sodium butyrate or 0.5 mu m trichostatin A are added to a pre-culture medium of the mature wheat embryos, so that the regeneration rate of mature wheat embryos of wheat materials CB 037, Fielder and Zhonguochun (Chinese Spring) can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method for improving the regeneration rate of mature wheat embryos. Background technique [0002] Wheat (Triticum aestivum L.) is the largest ration crop in the world, and about 35% to 40% of people use it as ration. With the continuous increase of the global population and the continuous development of biotechnology, it has become an important trend to improve wheat yield and quality and other agronomic traits through molecular design breeding. Gene function research requires the use of genetic engineering methods to introduce exogenous genes into wheat, and the low regeneration frequency of wheat tissue culture is an important limiting factor for genetic transformation. [0003] At present, the commonly used explants for genetic transformation of wheat include immature embryos, mature embryos, anthers and young spikes, etc. Among them, immature em...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 张宪省别晓敏李兴国高新起
Owner SHANDONG AGRICULTURAL UNIVERSITY
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