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Method for directionally preparing dextran

A technology of dextran and sugar solution, which is applied in the field of preparation of pharmaceutical raw materials, and can solve problems such as the application of highland barley polysaccharide extracts that have not yet appeared

Pending Publication Date: 2020-09-15
JING BRAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no application of highland barley polysaccharide extract in the preparation of dextran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Dextran sucrase extraction

[0028] Culture Leuconostoc enterococcus to the logarithmic phase, and inoculate the enzyme production medium according to the inoculum size of 0.4%. The medium is: sucrose 50g / L, yeast extract 12.5g / L, beef extract 20g / L, dihydrogen phosphate Potassium 1.0g / L, disodium hydrogen phosphate 3.6g / L, sterilized by high-pressure steam at 121°C for 15-20min; the culture conditions are 20-30°C, 100-200r / min, and the time is 20-30h. After the cultivation, the Leuconostoc enterococcus culture solution containing the crude enzyme solution was obtained. The crude enzyme solution is obtained by centrifugation in a centrifuge at a speed of 8000-10000r / min for 15-20 minutes. After centrifugation, the precipitate is discarded, and the supernatant is collected as the crude enzyme solution.

[0029] (2) Enzymolysis of barley extract

[0030] Use pure water to prepare 100 mL of highland barley extract mixture with a mass concentration of 10%, add 5% amyl...

Embodiment 2

[0038] (1) Dextran sucrase extraction

[0039] Culture Leuconostoc enterococcus to the logarithmic phase, and inoculate the enzyme production medium according to the inoculum size of 0.4%. The medium is: sucrose 50g / L, yeast extract 12.5g / L, beef extract 20g / L, dihydrogen phosphate Potassium 1.0g / L, disodium hydrogen phosphate 3.6g / L, sterilized by high-pressure steam at 121°C for 15-20min; the culture conditions are 20-30°C, 100-200r / min, and the time is 20-30h. After the cultivation, the Leuconostoc enterococcus culture solution containing the crude enzyme solution was obtained. The crude enzyme solution is obtained by centrifugation in a centrifuge at a speed of 8000-10000r / min for 15-20 minutes. After centrifugation, the precipitate is discarded, and the supernatant is collected as the crude enzyme solution.

[0040] Add 0.005% CaCl to the crude enzyme solution during storage 2 , stored at 4 ℃, can prolong its storage time.

[0041] (2) Enzymolysis of barley extract

...

Embodiment 3

[0050] (1) Dextran sucrase extraction

[0051] Culture Leuconostoc enterococcus to the logarithmic phase, and inoculate the enzyme production medium according to the inoculum size of 0.4%. The medium is: sucrose 50g / L, yeast extract 12.5g / L, beef extract 20g / L, dihydrogen phosphate Potassium 1.0g / L, disodium hydrogen phosphate 3.6g / L, sterilized by high-pressure steam at 121°C for 15-20min; the culture conditions are 20-30°C, 100-200r / min, and the time is 20-30h. After the cultivation, the Leuconostoc enterococcus culture solution containing the crude enzyme solution was obtained. The crude enzyme solution is obtained by centrifugation in a centrifuge at a speed of 8000-10000r / min for 15-20 minutes. After centrifugation, the precipitate is discarded, and the supernatant is collected as the crude enzyme solution. Add 0.005% CaCl to the crude enzyme solution during storage 2 , stored at 4 ℃, can prolong its storage time.

[0052] (2) Enzymolysis of barley extract

[0053] Us...

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Abstract

The invention discloses a method for directionally preparing dextran. In the method, a highland barley extract is adopted as a raw material, dextran products with different molecular weights can be directionally prepared by controlling a ratio of dextran sucrase to dextranase, and products such as dextran 10, dextran 20, dextran 40, dextran 70, dextran 100, dextran 1000 and the like can be prepared.

Description

technical field [0001] The invention belongs to the technical field of preparation of pharmaceutical raw materials, and in particular relates to a method for directional preparation of dextran. Background technique [0002] Highland barley is a plateau cereal crop, mainly used as food, fodder, and wine making. Studies have found that highland barley is rich in glucan, which is the crop with the highest β-glucan content among wheat crops. When extracting β-glucan from highland barley, the highland barley polysaccharide extract is obtained by water extraction, acid method, subtraction, etc., which contains a large amount of other polysaccharides in addition to a certain amount of β-glucan. Existing research mainly focuses on the extraction, preparation and functional research of highland barley β-glucan. Chinese patent application CN107226871A discloses a preparation method of highland barley β-glucan. After melting, the commercial active dry yeast of Saccharomyces cerevisia...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/08
CPCC12P19/14C12P19/08
Inventor 戴婷陈彦和魏朝丹田浩屈娜胡开群柳念邱睿
Owner JING BRAND
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