A kind of Rhizocystis intrarhizoides-biochar composite bacterial agent and its preparation method and application

A compound bacterial agent and biochar technology, applied in the field of agricultural microorganisms, can solve the problem of no research on the emergence of live peppers, and achieve the effects of promoting plant height and seedling emergence rate.

Active Publication Date: 2022-04-29
GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no research on the interaction between biochar and arbuscular mycorrhizal fungi in promoting seedling emergence of direct seeded peppers

Method used

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  • A kind of Rhizocystis intrarhizoides-biochar composite bacterial agent and its preparation method and application
  • A kind of Rhizocystis intrarhizoides-biochar composite bacterial agent and its preparation method and application
  • A kind of Rhizocystis intrarhizoides-biochar composite bacterial agent and its preparation method and application

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preparation example Construction

[0029] The present invention also provides the preparation method of the described Rhizocystis internatifiensis-biochar composite bacterial agent, comprising the following steps: mixing Rhizocystis internatifiensis and biochar to obtain a composite of Rhizocystis internatifiensis-biochar Bacteria. The present invention has no special limitation on the mixing method, and it is sufficient to adopt a conventional mixing method in the art to ensure uniform mixing.

[0030] The present invention also provides the application of the Rhizocystis interrabilis-biochar complex bacterial agent in improving the seedling emergence rate of direct-seeded peppers.

[0031] In the present invention, the application preferably includes the following steps: 1) adding the rhizoid cysts-biochar complex bacterial agent to the soil to obtain the soil to be sown; 2) sterilizing the pepper seeds and sowing to step 1 ) described in the soil to be sown.

[0032] In the present invention, the Rhizocyst...

Embodiment 1

[0036] S1: The dominant species of arbuscular mycorrhizal fungi in the tomato rhizosphere - Rhizophagus intraradices (Rhizophagus intraradices, Ri ) spores, the Ri spores were inoculated on the root system of host plants with white clover and corn, and planted in the river sand culture medium. Silk antiseptic.

[0037] S2: Collect rice straw waste, crush it to a particle size of 2mm, and put it into a carbon-making equipment, and perform an anaerobic pyrolysis reaction at a constant temperature of 450°C for 90 minutes to obtain rice straw biochar materials.

[0038] S3: Inoculate the microbial agent prepared in S1 into the biochar prepared in S2 at a ratio of 5:1 to obtain a Rhizocystis rhizoides-biochar composite bacterial agent.

[0039] S4: Add the Rhizocystis rhizoides-biochar composite bacterial agent prepared in S3 to the soil to obtain a seed sowing soil environment containing 17% of the Rhizocystis rhizoides-biochar composite bacterial agent.

[0040] S5: with 10% H ...

Embodiment 2

[0049] S1: Collect tomato root and soil samples, isolate, purify, and identify the dominant species of arbuscular mycorrhizal fungus-Rhizophagus intraradices (Ri), using river sand as the substrate and using two host plants, white clover Jointly culture and multiply Ri with corn to obtain Ri inoculant containing host root segments, spores (density 30 / g) and hyphae.

[0050] S2: Collect rice straw waste, pulverize it and put it into charcoal making equipment, and conduct an anaerobic pyrolysis reaction at a constant temperature of 450°C for 90 minutes to obtain rice straw biochar materials.

[0051] S3: Inoculate the microbial agent prepared in S1 into the biochar prepared in S2 at a ratio of 7:1 to obtain a Rhizocystis rhizoides-biochar composite microbial agent.

[0052] S4: The Rhizocystis rhizoides-biochar composite bacterial agent prepared in S3 was added to the soil to obtain a seed sowing soil environment containing 16% bacterial charcoal material.

[0053] S5: with 10%...

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Abstract

The present invention provides a rhizocystis internatica-biochar composite bacterial agent and its preparation method and application, belonging to the technical field of agricultural microorganisms, including rhizocystis internatina and biochar. The mass ratio to the biochar is (4-15): 1; the rhizocystis root is isolated from the rhizosphere of Solanaceae plants; the biochar is rice straw biochar. The Cystis rhizoides-biochar complex microbial agent of the invention can significantly promote the seedling emergence rate of direct seeding pepper seeds, and the seedling rate can be increased to 86.0%. Simultaneously, the Rhizocystis internata-biochar composite bacterial agent can also significantly promote the increase of plant height, stem diameter, aboveground biomass, root biomass and fresh fruit weight per plant of sown pepper seeds.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a Rhizocystis rhizoides-biochar composite bacterial agent and a preparation method and application thereof. Background technique [0002] In 1885, Frank, a German plant physiologist and forest scientist, first coined the term "mycorrhiza" (Fungus-root or Mycorrhiza). Mycorrhizae are a common plant symbiosis phenomenon in nature, which is a combination of mycorrhizal fungal hyphae in soil and vegetative roots of higher plants [0003] Arbuscular mycorrhizal (AM) has a symbiotic relationship with about 80% of terrestrial plants. It can absorb plant carbon, which in turn can enhance the absorption capacity of plant nutrients and plant stress resistance, and widely affects Plant-plant interactions and community structure. [0004] Arbuscular mycorrhizal fungi (AMF) are an ancient and rare species of symbiotic fungi in plant roots, which mainly exist ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10A01G7/06C12N1/14C12R1/645
CPCA01G18/10A01G7/06C12N1/14
Inventor 王永平邢丹王岩牟玉梅胡明文周鹏
Owner GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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