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Antigen-antibody compound vaccine for porcine epidemic diarrhea virus and preparation method thereof

A porcine epidemic diarrhea, antigen-antibody technology, applied in the directions of virus antigen components, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of insufficient immune protection of piglets, increased risk of infection with PEDV, etc.

Active Publication Date: 2020-09-22
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that traditional vaccines are not suitable for piglet immunization, resulting in insufficient immune protection of piglets during lactation and increased risk of PEDV infection, the invention provides a porcine epidemic diarrhea virus antigen-antibody complex vaccine and a preparation method thereof

Method used

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  • Antigen-antibody compound vaccine for porcine epidemic diarrhea virus and preparation method thereof
  • Antigen-antibody compound vaccine for porcine epidemic diarrhea virus and preparation method thereof
  • Antigen-antibody compound vaccine for porcine epidemic diarrhea virus and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0040] The specific operation steps of a preparation method of porcine epidemic diarrhea virus antigen-antibody complex vaccine are as follows:

[0041] (1) Preparation of viral antigen of porcine epidemic diarrhea virus

[0042] (1.1) Antigen species

[0043] The porcine epidemic diarrhea virus strain (SD strain) used for seedling production was isolated, identified, kept and supplied by the Animal Husbandry and Veterinary Research Institute of Anhui Academy of Agricultural Sciences. The strain was preserved in the China Center for Type Culture Collection on March 29, 2019 Preservation, address: Wuhan University, Wuhan, China, named: porcine epidemic diarrhea virus SD strain; deposit number is CCTCC NO: V201915.

[0044] (1.2) Preparation of porcine epidemic diarrhea virus antigen solution

[0045] The vero cells used for inoculation were cultured in 25cm 2 Cell culture flasks were cultured in DMEM medium containing 10% fetal bovine serum. Microscopically observe, when th...

Embodiment 2

[0068] Efficacy evaluation of porcine epidemic diarrhea virus antigen-antibody complex vaccine immunization on 3-day-old piglets

[0069] The 3-day piglets were immunized with the complex vaccine, divided into immunization group and control group, and injected with Houhai point, the injection dose was 3mL. Piglets in the experiment were fed with milk replacer. Blood was collected on the 1st, 6th, 12th and 18th days after immunization to observe the changes of serum antibodies, and the results were as follows: figure 1 It was shown that on the 6th day after immunization, the serum antibody level of piglets was significantly higher than that of the control group, indicating that the antigen-antibody complex vaccine can make newborn piglets produce protective antibodies early and improve the ability to resist virus infection.

Embodiment 3

[0071] Efficacy Evaluation of Porcine Epidemic Diarrhea Virus Antigen-antibody Complex Vaccine Immunization on 40-day-old Back-up Pigs

[0072] Get 20 back-up pigs in 40 days, be divided into inactivated vaccine immunization group and complex vaccine immunization group, every group 10, carry out inactivated vaccine and complex vaccine of the present invention respectively and carry out immunization 3 times, inoculation dose is 4mL, average Inoculation was done by Houhai point injection. Blood was collected on the 7th day after each vaccination, and the serum antibody titer was determined. The results were as follows: figure 2 Show, the average antibody titer of complex vaccine of the present invention is respectively 1:2 3.115 , 1:2 4.874 and 1:2 6.874 , the average antibody titers of inactivated vaccines were 1:2 2.737 , 1:2 4.737 and 1:2 6.874 , complex vaccination can induce higher levels of serum antibodies in the early stage than inactivated vaccine.

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Abstract

The invention discloses a virus antigen-antibody compound vaccine for porcine epidemic diarrhea and a preparation method of the virus antigen-antibody compound vaccine, and belongs to the technical field of veterinary vaccines. The antigen-antibody compound vaccine is obtained by fully mixing a porcine epidemic diarrhea virus antigen and a porcine epidemic diarrhea virus antibody according to a volume ratio of 1: 5, penicillin with a final concentration of 1000 UI / mL and streptomycin with a final concentration of 1000 [mu]g / mL are added, and the vaccine is obtained by incubating at a temperature of 37 DEG C for 2 h. The antigen-antibody compound vaccine does not need to inactivate the antigen, so that live viruses in the antigen-antibody compound vaccine are effectively protected, and immune organs are induced to generate more immune germinal centers. The antibody in the compound vaccine plays a role in delaying virus release, and the Fc fragment of the antibody enables the compound tobe captured by antigen presenting cells more easily, so that immune response is caused. The virus antigen-antibody compound vaccine can break through a maternal antibody and induce a high-titer antibody, and has a good immune effect and a good challenge protection rate on suckling piglets, replacement pigs, sows and offspring piglets thereof.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccines, and in particular relates to a porcine epidemic diarrhea virus antigen-antibody complex vaccine and a preparation method thereof. [0002] technical background [0003] Porcine epidemic diarrhea virus (PEDV) is an acute intestinal infectious disease that can cause pigs. The main symptoms of infected pigs are severe diarrhea, vomiting and dehydration; the virus only infects pigs, and pigs of all ages and breeds are susceptible. The infection mode of PEDV is mainly through the digestive tract infection through the feces of infected pigs, among which the suckling piglets suffer the most serious damage, and the mortality rate is as high as 80-100%. The virus infection is the main disease that causes early death of piglets one. The emergence of the disease was first reported in my country in 1976, and PEDV infection broke out in many provinces of my country after 2010, causing huge econom...

Claims

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Application Information

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IPC IPC(8): A61K39/385A61K39/215A61P31/14C12N7/00C07K16/10C07K16/06C12R1/93
CPCA61K39/385A61K39/12A61P31/14C12N7/00C07K16/10C07K16/06A61K2039/552C12N2770/20034A61K2039/6056C12N2770/20051C07K2317/35Y02A50/30
Inventor 沈学怀潘孝成刘华赵瑞宏张丹俊翟银建戴银胡晓苗周学利侯红艳
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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