Mercury ion detection method
A detection method, a technology for mercury ions, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemically reacting materials, etc., can solve high background and false positive results, etc. problem, to achieve the effect of improving sensitivity, intuitive and easy detection, and reducing cost
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Embodiment 1
[0025] Draw the standard curve: Prepare mercury ion standard solutions with gradient concentrations, the solution concentrations are: 0.05nM, 0.1nM, 0.5nM, 1nM, 2nM, 4nM, 5nM, 10nM. Mix an appropriate amount of the above standard solution with 100 nM sequence T, 50 nM DNA complex and 100 nM fuel chain F, respectively, at 37 ° C in a solution containing 5 mM MgCl 2 10mM Tris-HCl buffer solution (pH 7.5) and incubated for 120 minutes. Then, 30 nM hemin and 25 mM KCl were added to the above solution, and finally, 600 μM 3,3',5,5'-tetramethylbenzidine and 10 mM H 2 o 2 Tris-HCl solution (pH 5.5) was used for color reaction. The UV-vis absorption spectrum was detected by a UV-vis spectrophotometer. Such as figure 1 As shown in A, the absorbance intensity varies with Hg 2+ increased with increasing concentration. Absorbance intensity and Hg at 0.05nM to 2nM 2+ A good linear correlation was shown between concentrations ( figure 1 B). The regression equation can be expressed ...
Embodiment 2
[0029] Collect water samples from tap water, streams, and springs. All water samples were filtered through a 0.22 μm membrane to remove solid residues prior to measurement. The detection process is the same as in Example 1, and the detection results are as shown in Table 1. In the actual water sample, Hg 2+ Concentrations of tap water, stream water and spring water. The recovery rate increased from 91.6% to 105.3%. These results show great promise in the detection of Hg ions in real environmental water samples.
[0030] Table 1. Test results of real water samples
[0031]
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