Mercury ion detection method

A detection method, a technology for mercury ions, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemically reacting materials, etc., can solve high background and false positive results, etc. problem, to achieve the effect of improving sensitivity, intuitive and easy detection, and reducing cost

Active Publication Date: 2020-09-22
CHONGQING TECH & BUSINESS UNIV
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods are driven by the free energy of base pair formation, which can lead to relatively high background and false positive results
[0004] Thymine-Hg 2+ - Thymine (T-Hg 2+ -T) Report on mercury ion detection method combined with coordination chemistry and entropy-driven catalytic chromogenic reaction

Method used

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  • Mercury ion detection method
  • Mercury ion detection method
  • Mercury ion detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Draw the standard curve: Prepare mercury ion standard solutions with gradient concentrations, the solution concentrations are: 0.05nM, 0.1nM, 0.5nM, 1nM, 2nM, 4nM, 5nM, 10nM. Mix an appropriate amount of the above standard solution with 100 nM sequence T, 50 nM DNA complex and 100 nM fuel chain F, respectively, at 37 ° C in a solution containing 5 mM MgCl 2 10mM Tris-HCl buffer solution (pH 7.5) and incubated for 120 minutes. Then, 30 nM hemin and 25 mM KCl were added to the above solution, and finally, 600 μM 3,3',5,5'-tetramethylbenzidine and 10 mM H 2 o 2 Tris-HCl solution (pH 5.5) was used for color reaction. The UV-vis absorption spectrum was detected by a UV-vis spectrophotometer. Such as figure 1 As shown in A, the absorbance intensity varies with Hg 2+ increased with increasing concentration. Absorbance intensity and Hg at 0.05nM to 2nM 2+ A good linear correlation was shown between concentrations ( figure 1 B). The regression equation can be expressed ...

Embodiment 2

[0029] Collect water samples from tap water, streams, and springs. All water samples were filtered through a 0.22 μm membrane to remove solid residues prior to measurement. The detection process is the same as in Example 1, and the detection results are as shown in Table 1. In the actual water sample, Hg 2+ Concentrations of tap water, stream water and spring water. The recovery rate increased from 91.6% to 105.3%. These results show great promise in the detection of Hg ions in real environmental water samples.

[0030] Table 1. Test results of real water samples

[0031]

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Abstract

The invention provides a mercury ion detection method combining thymine-Hg < 2 + >-thymine (T-Hg < 2 + >-T) coordination chemistry and entropy-driven catalytic chromogenic reaction. The entropy-drivencatalytic reaction is chemically induced by T-Hg < 2 + >-T coordination, so that a G-rich sequence is released. A chlorhematin / G-quadruplex structure can be formed with the help of chlorhematin, a DNA mimic enzyme with catalase activity is formed, a substrate 3, 3 ', 5, 5'-tetramethyl benzidine is oxidized, the color is changed from colorless to blue, and a good linear relation exists between 0.05 nM and 2 nM. The method can be used for on-site analysis of the concentration of Hg < 2 + > in water.

Description

technical field [0001] The invention relates to the field of detection of mercury ions, especially the thymine-Hg 2+ - Thymine (T-Hg 2+ -T) The field of mercury ion detection method combined with coordination chemistry and entropy-driven catalytic chromogenic reaction. Background technique [0002] Mercury pollution has become a global problem of human health and environmental protection. Although mercury occurs naturally in the environment, human activities lead to mercury entering the environment, such as mining, burning of fossil fuels, and incineration of solid waste, etc. Trace Hg 2+ May bioaccumulate in humans by binding to sulfhydryl-containing molecules. Hg in the human body 2+ Even at very low concentrations it can cause neurological disorders, kidney disease and other ailments due to its high toxicity. Therefore, it is necessary to develop a method for detecting Hg in the field of food safety and environmental protection 2+ sensitivity method. Colorimetry h...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/78G01N21/33
Inventor 云雯王崇均王瑞琪胡媛刘秋林刁晓琦
Owner CHONGQING TECH & BUSINESS UNIV
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