Application of a plasma metabolic marker related to the diagnosis of tic syndrome in children in the preparation of a diagnostic kit for tic syndrome in children
A technology of multiple tics and metabolic markers, applied in the field of metabolomics, to achieve the effect of improving possibility and feasibility, improving sensitivity and specificity, and simple operation
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[0039] 1. Test materials: chromatographic grade acetonitrile (Merck), methanol (TEDIA); ammonium acetate (Sigma); standard L-glutamic acid hydrochloride, D-ornithine hydrochloride, L-ornithine hydrochloride Salt, D-pipercolic acid, D-proline, L-arginine, L-homoproline, L-carnitine (purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.); AB Triple TOF 5600 mass spectrometer (purchased from ABSCIEX, Framingham, MA, USA); Agilent 1290 Infinity LC ultra-high pressure liquid chromatograph (purchased from Agilent Technologies, Santa-Clara, California, USA); Agilent 6460 Triplequadrupole mass spectrometer (purchased from Agilent Technologies, Santa -Clara, California, USA); low-temperature high-speed centrifuge (Eppendorf5430R); chromatographic column: ACQUITY UPLC HSS T3 1.8μm, 2.1×100mm column (Waters); Agilent ZORBAX Hilic Plus 1.8μm, 50×2.1mm (Aglient, USA) .
[0040] 2. Sample collection and sample data collation:
[0041] The present invention uses a total of 49 ca...
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