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3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone and method for detecting acetoin content in exhaled breath by using same

A technology of dinitrophenylhydrazone and dinitrophenylhydrazine, which is applied in the field of acetoin content and 3-hydroxy-2-butanone-2, can solve the problems of unquantifiable detection content and complex sample components, etc. Achieve the effect of convenient and safe operation and processing, simple synthesis route and high purity

Pending Publication Date: 2020-10-02
必睿思(杭州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the volatile components of exhaled breath, the sample components are complex, and most of the existing detections in human exhaled breath are qualitative detections, and cannot quantitatively detect the content

Method used

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  • 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone and method for detecting acetoin content in exhaled breath by using same
  • 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone and method for detecting acetoin content in exhaled breath by using same
  • 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone and method for detecting acetoin content in exhaled breath by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Prepare a certain amount of 2,4-dinitrophenylhydrazine (DNPH) reagent in a beaker, add acetonitrile to dissolve, make DNPH supersaturated solution, transfer the upper layer solution to another beaker (the upper layer solution is the saturated DNPH solution ), in this saturated DNPH solution, add concentrated hydrochloric acid (concentrated hydrochloric acid is laboratory concentrated hydrochloric acid, content between 36%-38%), obtain acidic saturated 2,4-dinitrophenylhydrazine solution, acidic saturation 2, 4-Dinitrophenylhydrazine solution contains 0.005% volume fraction of hydrogen ions.

[0041] Add 3-hydroxy-2-butanone into acetonitrile and dissolve it to form a 3-hydroxy-2-butanone solution. The acetonitrile contains 0.005% volume fraction of hydrogen ions. Put the above acidic saturated DNPH solution at a constant temperature of 60°C In a water bath, after the temperature of the acidic saturated DNPH solution reaches 60°C, add 3-hydroxy-2-butanone solution into t...

Embodiment 2

[0051] The molar ratio of the amount of 3-hydroxy-2-butanone added to the amount of 2,4-dinitrophenylhydrazine is 1:2, and the others are the same as in Example 1. The yield of the reaction product was measured to be 97.6%, and the purity of the purified 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone was 99.42%.

Embodiment 3

[0053] The molar ratio of the amount of 3-hydroxy-2-butanone added to the amount of 2,4-dinitrophenylhydrazine is 1:3, and the others are the same as in Example 1. The yield of the reaction product was measured to be 99.7%, and the purity of the purified 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone was 98.83%.

[0054] Preparation of Sorbent Sampling Tubes

[0055] 2,4-dinitrophenylhydrazine (DNPH) is configured with acetonitrile into a coating solution containing 3% of the derivatizing reagent; then 200 mg of stationary phase filler is filled on a blank adsorption column; finally the coating solution is coated with On the adsorption column filled with stationary phase, the coating method is carried out by direct coating method. Rinse the blank adsorption column with acetonitrile before coating, and remove the rinsed acetonitrile reagent by the negative pressure solid phase extraction system, then apply 3% coating solution directly on the adsorption column, and use negativ...

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Abstract

The invention provides 3-hydroxyl-2-butanone-2,4-dinitrophenylhydrazone and a method for detecting acetoin content in exhaled breath by using the 3-hydroxyl-2-butanone-2,4-dinitrophenylhydrazone, andbelongs to the field of biochemical engineering. The invention relates to 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone, of which the structural formula of the compound is shown as a formula (I), and the 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone is a new compound and can be used for detecting the acetoin content in exhaled breath of a human body, so that a new application field is developed.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and particularly relates to 3-hydroxy-2-butanone-2,4-dinitrophenylhydrazone and a method for detecting acetoin content in exhaled air by using it. Background technique [0002] The exhaled air of the human body is directly exhaled from the lungs. Pauling et al. first collected the exhaled air of normal people in 1971, and detected 250 kinds of volatile organic compounds. [0003] The volatile organic compounds in the exhaled breath of the human body are closely related to the diagnosis of diseases. These volatile organic compounds contain acetoin (also known as 3-hydroxy 2-butanone). If acetoin is detected in the exhaled breath, it means that the A person may suffer from a certain disease such as lung cancer, and the level of acetoin in the exhaled breath can represent the severity of the disease to a certain extent, and the diagnosis of the disease requires follow-up further diagnosis. Howev...

Claims

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Application Information

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IPC IPC(8): C07C251/76C07C249/16G01N30/02G01N30/06
CPCC07C251/76G01N30/06G01N30/02G01N2030/067
Inventor 温铁芳赵伟军周伟汪小知张厚德郝雷段兴斌
Owner 必睿思(杭州)科技有限公司
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