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Application of drosophila melanogaster Hsp22 protein in preparation of anti-tumor drug

An anti-tumor and protein technology, applied in the field of medicine, can solve problems such as the urgent demand for anti-cancer drugs

Active Publication Date: 2020-10-09
AFFILIATED HOSPITAL OF GUILIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The RAS oncogene is activated in 30% of cancer types, which will lead to the proliferation and transformation of tumor cells. Currently, there is no effective inhibitor for this protein (encoded by the RAS gene), so there is an urgent need for anti-cancer drugs

Method used

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  • Application of drosophila melanogaster Hsp22 protein in preparation of anti-tumor drug
  • Application of drosophila melanogaster Hsp22 protein in preparation of anti-tumor drug
  • Application of drosophila melanogaster Hsp22 protein in preparation of anti-tumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Treatment of Drosophila Hsp22 Protein to Ras Tumor Drosophila Model

[0023] (1) Construction of Drosophila tumor model

[0024] ①Construction of RasV12; scrib- / - Drosophila tumor model (overexpression of oncoprotein Ras and recessive homozygous mutation of tumor suppressor gene scrib- / -)

[0025] The promoter scrib-GAL4, which is selectively expressed in the eye disc and abdominal nerve cord of Drosophila larvae, was crossed with RasV12 fruit flies labeled with GFP fluorescence (the fruit flies were donated by Professor Xue Lei from the School of Life Sciences, Tongji University, Shanghai ), the obtained F1 generation is RasV12; scrib- / - tumor Drosophila (overexpression of the oncoprotein Ras and scrib-recessive homozygous mutation).

[0026] ② Construction of hsp22 overexpressed RasV12; scrib- / - tumor Drosophila

[0027] Cross hsp22 overexpression flies (genotype: + / cyo; hsp22 OE / TM2) with RasV12 flies (genotype: RasV12 / sco; TM3 / +), and screen RasV12 / cyo; ...

Embodiment 2

[0034] Example 2 Drosophila Hsp22 protein treatment of mouse liver cancer

[0035] (1) Construction of monoclonal bacteria

[0036] The whole gene synthesis of the Drosophila Hsp22 protein gene sequence (the amino acid sequence is shown in SEQ ID NO: 1, and the nucleotide sequence is shown in SEQ ID NO: 2), after being digested with BamHI and XhoI, inserted into the pET28a vector to obtain After the recombinant plasmid is sequenced correctly, the recombinant plasmid is transformed into the host bacterium Escherichia coli to obtain monoclonal bacteria.

[0037] (2) Drosophila Hsp22 protein expression

[0038]Configure the culture medium, sterilize, and apply the monoclonal bacteria obtained in step (1) to a kanamycin (KA) plate at night, overnight. The next day, select monoclonal bacteria into 10ml of KA (50ug / ml) liquid medium, and overnight at 250rpm and 37°C. On the third day, transfer 1% overnight bacteria to 1000ml of liquid medium, expand and shake the bacteria at 250r...

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Abstract

The invention belongs to the field of medicines, and particularly relates to an application of drosophila melanogaster Hsp22 protein in preparation of anti-tumor drug. The pharmacological activity experiment part evaluates the treatment effect of the drosophila melanogaster Hsp22 protein on a Ras tumor drosophila melanogaster model and the treatment effect on a mouse tumor model, and finds that the drosophila melanogaster Hsp22 protein has good antitumor activity. The drosophila melanogaster Hsp22 protein can be used for developing novel anti-tumor drugs and has important medical prospects andeconomic value.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of a fruit fly Hsp22 protein in the preparation of anti-tumor drugs. Background technique [0002] Cancer is a malignant disease with a very high mortality rate. It is difficult to treat and brings a heavy burden to patients and families. In recent years, the incidence of cancer in our country has increased significantly, making cancer prevention and treatment face a severe situation. According to relevant research reports, in the 1970s, cancer in my country increased from 10.13% to 22.32%, and the death rate increased by 82.11%. Cancer ranks first in urban deaths and second in rural areas. Especially in today's aging population, smoking, dietary changes, microbial infections, obesity, reduced activity, poor work and rest, and other factors are the main causes of cancer. Currently, the top ten cancers in my country are: lung cancer, gastric cancer, colorecta...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K35/64A61P35/00
CPCA61K38/1767A61K35/64A61K9/0019A61K9/0043A61P35/00
Inventor 李清华韦载娲王文晶廖儒佳莫靖欣田宁石芳李美营
Owner AFFILIATED HOSPITAL OF GUILIN MEDICAL UNIV
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