Preparation method of agonist for improving fish sperm motility

A fish sperm and agonist technology, applied in biochemical equipment and methods, cell culture active agents, animal cells, etc., can solve the problems of not being fully utilized, and achieve the goal of easy popularization, use, and production Effect

Inactive Publication Date: 2020-10-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its distinctive smell, it has been underutilized for a long time

Method used

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  • Preparation method of agonist for improving fish sperm motility
  • Preparation method of agonist for improving fish sperm motility
  • Preparation method of agonist for improving fish sperm motility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method for improving fish sperm motility agonist,

[0035] Prepared by:

[0036] (1) Take gonads of Atlantic salmon whose gonads are in the V stage of development, and put them into Hanks solution at 28°C with 2% penicillin for 45 minutes;

[0037] (2) Isolate the mesenchymal cells of Atlantic salmon gonads, and wash them twice with Hanks solution containing 2% streptomycin;

[0038] (3) Add HHBS cell culture medium, incubate at 28°C for three days, and subculture according to the growth state of the cells at a ratio of 1:2 or 1:3, transfer the subcultured cell liquid to a centrifuge tube, and centrifuge remove the supernatant;

[0039] (4) Add 3 mL of hyaluronidase into the centrifuge tube, shake vigorously every 2 minutes at 28°C, and after shaking continuously for 4 to 5 times, add 5 mL of cell culture medium, and take the cultured cell liquid at 2000r / min Centrifuge to remove the supernatant. ;

[0040] (5) filter the supernatant with a cell sieve...

Embodiment 2

[0043] A preparation method for improving fish sperm motility agonist is characterized in that it comprises the following steps:

[0044] (1) Take the gonads of turbot whose gonads are in the V stage of development, and soak them in 28°C Hanks solution with 2% penicillin for 45 minutes;

[0045] (2) Separating the interstitial cells of turbot gonads and washing them twice with Hanks solution containing 2% streptomycin;

[0046] (3) Add HHBS cell culture medium, incubate at 28°C for three days, and subculture according to the growth state of the cells at a ratio of 1:2 or 1:3, transfer the subcultured cell liquid to a centrifuge tube, and centrifuge remove the supernatant;

[0047] (4) Add 3 mL of hyaluronidase into the centrifuge tube, shake vigorously every 2 minutes at 28°C, and after shaking continuously for 4 to 5 times, add 5 mL of cell culture medium, and take the cultured cell liquid at 2000r / min Centrifuge to remove the supernatant. ;

[0048] (5) filter the supernat...

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PUM

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Abstract

The invention relates to a preparation method of an agonist for improving fish sperm motility. The method comprises the following steps: (1) taking the fish gonads in a development stage V; (2) separating interstitial cells of the fish gonad; (3) adding an HHBS cell culture solution; (4) adding 3mL of hyaluronidase into a centrifugal tube, adding 5 mL of the cell culture solution after concussion,and obtaining a supernatant by centrifugation; (5) filtering the supernatant with a cell sieve; (6) respectively adding DMSO, glutathione and cysteine to the filtered cell supernatant, blowing, beating, uniformly mixing, and carrying out cryopreservation at the temperature of -20 DEG C so as to obtain the product. The method has the advantages of good application effect, low cost and easiness inprocessing and production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of an agonist for improving fish sperm motility. Background technique [0002] The testis tissue of mature male fish is white in color, distributed on the ventral side of the fish body, accounting for about 7% of the live body weight, and its main component is sperm. Due to its distinctive smell, it has been underutilized for a long time. But now fish gonads have become an important pharmaceutical raw material, and medicines made from fish gonads are contributing to the treatment of diseases. The main components of fish gonads are nucleoproteins, enzymes and various trace elements (more manganese, zinc, copper, molybdenum, etc.). Nucleoprotein is a white fibrous substance separated and extracted from gonads, mainly composed of deoxyribonucleic acid DNA and basic protein (protamine), of which DNA accounts for about 2 / 3, and protamine accounts for 1 / 3...

Claims

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Application Information

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IPC IPC(8): C12N5/076
CPCC12N5/061C12N2500/62C12N2500/32C12N2500/80C12N2501/999
Inventor 迟良董焕声刘焕奇
Owner QINGDAO AGRI UNIV
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