Fermentation liquor pretreatment method coupled with flocculation and adsorption

Abstract

The invention discloses a fermentation liquor pretreatment method coupled with flocculation and adsorption, belongs to the field of pretreatment and purification of bio-based product fermentation liquor, and aims at solving the technical problems that an existing flocculation pretreatment technology is low in acid-base applicability, the floc is small and difficult to filter, a large amount of filter aids needs to be added, part of soluble impurities in fermentation liquor cannot be effectively removed, and the subsequent separation and purification difficulty is increased. The method comprises the following steps: 1, preparing an inorganic polymer flocculation solution; 2, preparing a cross-linked organic polymer flocculation solution; 3, preparing a composite flocculant solution; 4, carrying out flocculation and coagulation; 5, performing activated carbon adsorption; and 6, carrying out solid-liquid separation. According to the provided inorganic-organic composite flocculant preparation technology, through an efficient condensation reaction, carboxyl, an aldehyde group, hydroxyl and other active groups are introduced, rich chain-shaped and cyclic structures are generated throughcrosslinking modification, the electrification condition of the flocculant is improved after the flocculant is further compounded with an inorganic flocculant, the flocculation capacity is high, the scum yield is low, and flocs are easy to separate.

Description

technical field [0001] The invention belongs to the field of pretreatment and purification of fermentation liquid of bio-based products; in particular, it relates to a method for pretreatment of fermentation liquid coupled with flocculation and adsorption. Background technique [0002] Microbial fermentation technology is the core of bioengineering. Whether traditional fermentation products such as antibiotics, amino acids, or modern genetic engineering products such as vaccines and human proteins, they all need to be produced through fermentation technology. Fermentation broth pretreatment is the first step in the separation and purification process of fermentation products. By separating cells, bacteria and other suspended particles (cell debris, nucleic acid and protein precipitates) and some soluble impurities (pigments, etc.), the physical properties of the fermentation broth are changed. In order to facilitate the subsequent separation and purification work. [0003] ...

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the technical problems that the existing flocculation pretreatment technology has low applicability to acid and alkali, small flocs, difficult to filter, a large amount of filter aids need to be added, and some soluble impurities in the fermentation broth cannot be effectively removed, which increases the difficulty of subsequent separation and purification. , and provides a fermentation broth pretreatment method coupled with flocculation adsorption