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Potosia brevitarsis larva alkali melting extraction protein and application thereof

A technology of beetles and larvae of white star flower, which is applied in the field of agricultural biology and can solve the problem of rare insect protein

Active Publication Date: 2020-10-20
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on the larvae of the white star beetle at home and abroad mainly focus on the two aspects of feeding management and control technology, and there are few studies on the protein of the white star beetle larvae and its efficacy mechanism
In addition, there are many studies on the classification and extraction of animal and plant proteins at home and abroad, and the system is relatively mature, while the research reports on the classification and extraction of insect proteins and their anti-inflammatory properties after classification are relatively rare

Method used

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  • Potosia brevitarsis larva alkali melting extraction protein and application thereof
  • Potosia brevitarsis larva alkali melting extraction protein and application thereof
  • Potosia brevitarsis larva alkali melting extraction protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Extraction of the anti-inflammatory protein of the larvae of the white star beetle

[0029] 1. Extraction

[0030] Salt extraction method: Accurately weigh 50.00g of defatted Beetle larvae sample, add 750mL of 1.5% NaCl solution at a ratio of 1:15 (g / mL), stir in a water bath for 1.5h at a constant temperature of 55°C, and rotate at a speed of 8000r / min Centrifuge for 10 min and take the supernatant. Slowly pour 8.00 mol / L hydrochloric acid solution into the supernatant until no more precipitation occurs, centrifuge at 8000r / min for 10min, take the precipitate, and freeze-dry it for later use.

[0031] Acid extraction method: Accurately weigh 50.00g of defatted Beetle larvae sample, add 750mL of 0.1mol / L acetic acid solution at a ratio of 1:15 (g / mL), stir in a water bath for 1.5h at a constant temperature of 55°C, and rotate at 8000r / min centrifuged for 10 min, the supernatant was taken, and the precipitate was repeatedly extracted once. The supernatant wa...

Embodiment 2

[0044] Example 2 Optimization of Extraction Conditions for the Anti-inflammatory Protein of Beetle larvae

[0045] Taking the protein extraction rate and total anti-inflammatory ability of the larvae of the white star beetle as the measurement index, the NaOH concentration (g / mL), solid-liquid ratio (defatted insect powder: NaOH solution = M:V), hydrolysis time (h), and precipitation were investigated. Effect of pH and hydrolysis temperature (°C) on metrics.

[0046] (1) Condition optimization of solid-liquid ratio (defatted insect powder: NaOH solution=M:V)

[0047] Under the condition of 55°C, the extraction time was 1.5h, the NaOH concentration in the extraction system was 1.5% (g / mL), the pH value of the precipitation was 4, and the solid-liquid ratio (defatted insect powder: NaOH solution = M:V) was 1:5, 1 Under the conditions of :10, 1:15, 1:20, and 1:25, measure each measurement index.

[0048] like Figure 8 It was shown that when the ratio of the mass of defatted i...

Embodiment 3

[0061] Example 3 Detection of anti-inflammatory effect of anti-inflammatory protein of white star beetle larvae

[0062] Take out the freeze-dried sample components, weigh an appropriate amount and dissolve them in distilled water. Take out the dialysis bag, soak it in distilled water and wash it, inject 5mL of sample solution into the dialysis bag, clamp and seal both ends of the dialysis bag, put it into a beaker filled with distilled water, place it on a rotator, change the water every 20 minutes, and detect it with silver nitrate. Until no white precipitate appears in the beaker, it proves that the dialysis is complete. Pour out the sample solution in the dialysis bag, and freeze dry it for storage.

[0063] 1. The effect of anti-inflammatory protein from the larvae of the white star beetle on the production of NO in RAW264.7 cells induced by LPS

[0064] After optimizing the extraction conditions, the larval anti-inflammatory proteins were extracted. The extracted crud...

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Abstract

The invention relates to the technical field of agricultural living things, in particular to a potosia brevitarsis larva alkali melting extraction protein and an application thereof. The obtained potosia brevitarsis larva alkali melting extraction protein has the effect of resisting inflammation, the anti-inflammatory action of inflammation resisting proteins on NO, TNF-alpha and IL-1 proteins within a certain range is in concentration dependence, and when the mass concentration of the inflammation resisting protein is 15mg / mL, the anti-inflammatory action is the best.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to an alkali fusion protein extracted from the larvae of the white star beetle and its application. Background technique [0002] The larvae of the white star beetle are fed with straw, rotten vegetable leaves and mushroom chaff, and are very effective converters of agricultural waste. White star flower beetle larva is a traditional Chinese medicinal material. It has the functions of breaking blood stasis, dispelling stagnation, relieving pain, and detoxifying. It can treat tetanus, nebula, and erysipelas in children. With the help of modern medicine and detection and analysis methods, it can be known that the larvae of the white star beetle have significant effects in anti-bacterial virus, anti-tumor and anti-oxidative aging. At present, domestic and foreign reports on the larvae of the white star beetle mainly focus on the two aspects of feeding management and control te...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30A61K38/17A61P29/00
CPCA61P29/00C07K14/43563A61K38/00
Inventor 石冬冬李秀梅秦铭潞张海英满晨
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES