Application of osnrt2.3b in Improving Yield and Rice Quality

A high-quality rice technology, applied in the field of genetic engineering, can solve the problems of poor yield increase effect, large application amount, and high grain production cost

Active Publication Date: 2022-08-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country's traditional agriculture attaches great importance to the use of organic fertilizers, which can promote crop yields, improve quality, and improve soil quality. However, there are disadvantages such as slow fertilizer efficiency, low nutrient content, labor-intensive application, and poor yield-increasing effects (Zhou Jiangming, 2012)
Although inorganic fertilizers have the advantages of fast increase in production, high nutrient content, and low dosage, the current excessive use by farmers has caused problems such as high food production costs, soil quality degradation, and serious agricultural non-point source pollution.

Method used

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  • Application of osnrt2.3b in Improving Yield and Rice Quality
  • Application of osnrt2.3b in Improving Yield and Rice Quality
  • Application of osnrt2.3b in Improving Yield and Rice Quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Construction of OsNRT2.3b gene overexpression vector

[0017] According to the full-length cDNA sequence of the OsNRT2.3b gene (obtained from the NCBI website), primers for the amplification of the full-length ORF were designed with the software Primer 5.0, and two different enzyme cleavage sites, SpeI and BglII, were designed at the 5' ends of the primers, respectively. For a different expression vector pCAMBIA1302, the sense strand was overNRT2.3b-F: 5'-caACTAGTGCTACCACGTGTTGGAGATG-3', the antisense strand overNRT2.3b-R: 5'-gaAGATCTGAGCAAACCACCAACAAG-3', purchased from the National Institute of Agrobiological Sciences ( The NIAS) OsNRT2.3b gene cDNA full-length cloning plasmid was used as the template to amplify the open reading frame (ORF) sequence. The PCR product containing the target gene was recovered and purified, and then connected to pMD19-Tcloning Vector, verified by enzyme digestion and sequenced. The correctly sequenced plasmids were digested wit...

Embodiment 2

[0018] Example 2 OsNRT2.3b overexpression plant acquisition and molecular biology identification

[0019] GM T 0 After the substitute seedlings were screened by hygromycin or GUS staining, 50 transgenic positive lines of OsNRT2.3b-Ox (35S promoter) were obtained, and the obtained transgenic seedlings were transplanted into the field for breeding to obtain T 0 Substitute seeds. Among the positive lines, we obtained 18 OsNRT2.3b-Ox transgenic lines whose phenotype was significantly different from that of the wild type. In order to further determine whether the target gene was enhanced in these lines, the T 0 The total RNA was extracted from the leaves of the transgenic seedlings for preliminary RT-PCR identification, and several lines with enhanced expression of the target gene were obtained.

[0020] To verify the genetic stability of the transgenic lines, the T 0 Substitute seeds and wild-type seeds were hydroponic and seedlings were germinated for 2-3 days and then transfe...

Embodiment 3

[0021] Example 3 Experimental Design

[0022] The experiment was set up with three fertilizer application levels (M: organic fertilizer treatment, NPK: inorganic fertilizer treatment, NPKM: organic fertilizer), and two fertilizer gradients (1: low, 2: high), namely organic low (M1) and organic high (M2) ), inorganic low (NPK1), inorganic high (NPK2), inorganic fertilizer + organic fertilizer combined application low (NPKM1), inorganic fertilizer + organic fertilizer combined application high (NPKM2), a total of 6 treatments, each treatment set 3 replicates, A total of 18 cells, arranged in random blocks, each cell has an area of ​​3.75m 2 (3m×1.25m), the depth of the plot is 1.2 m, and the plots are separated by bricks and concrete to prevent the exchange of water and fertilizer between plots. Nitrogen fertilizer was applied according to base fertilizer: tiller fertilizer: ear fertilizer = 5: 2: 3, and urea (containing N 46%) was applied. Among them, N ≥ 10% in organic ferti...

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Abstract

The invention discloses the application of OsNRT2.3b in improving yield and rice quality. Under different fertilizer treatment conditions, the overall OsNRT2.3b overexpression material had higher effective tillering, single ear weight, single plant yield, brown rice rate, and milled rice rate than that of Nipponbare wild type; water content and aspect ratio were higher than those of Nipponbare wild type, chalky Both the white grain rate and chalkiness were lower than those of the Nipponbare wild type.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to the application of OsNRT2.3b in improving yield and rice quality. Background technique [0002] Rice (Oryza sativa L.) is an important staple food for billions of people (Zeng, et al., 2017). As a large agricultural country, my country is one of the earliest countries in the world to plant rice, and is currently one of the most important food crops in my country (Chen Ligang, 2019, Situ Song, et al., 2000). For a long time, in order to improve rice production, farmers have always regarded fertilization as the most important physicochemical technical measure for rice production. my country's traditional agriculture attaches great importance to the use of organic fertilizers, which can promote crop yield, improve quality, and improve soil quality. . Although inorganic fertilizers have the advantages of rapid yield increase, high nutrient content, and low dosage, the cur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/84A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 范晓荣赵利梅钱开芸
Owner NANJING AGRICULTURAL UNIVERSITY
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