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Method for obtaining molecular marker related to malformation trait of gynogenesis hypophthalmichthys nobilis and application of molecular marker

A technology related to molecular markers and gynogenesis, applied in the fields of aquatic animal genetics and molecular marker-assisted selection breeding, can solve the problems of no SNP molecular markers reported, and achieve accurate and reliable identification results, simple operation, and the effect of solving blindness.

Active Publication Date: 2020-10-27
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no reports on SNP molecular markers related to deformity traits of gynogenetic bighead carp and its application

Method used

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  • Method for obtaining molecular marker related to malformation trait of gynogenesis hypophthalmichthys nobilis and application of molecular marker
  • Method for obtaining molecular marker related to malformation trait of gynogenesis hypophthalmichthys nobilis and application of molecular marker
  • Method for obtaining molecular marker related to malformation trait of gynogenesis hypophthalmichthys nobilis and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The SNP markers of the present invention are derived from the simplified genome data constructed by the gynogenetic bighead carp 2b-rad method in our laboratory, and the loci significantly related to the deformity traits of bighead carp obtained through analysis.

[0039] (1) The gynogenetic bighead carp used in the experiment was collected from the Zhangdu Lake fishery in Xinzhou District, Wuhan. The bighead carp used was one month old. Six normal bighead carp and six deformed bighead carp were selected. Genomic DNA was extracted from the caudal fin of The 2b-rad method for library construction is mainly divided into enzyme digestion, ligation, amplification and recovery.

[0040] Digestion:

[0041] 2b-RAD bank building was carried out on 6 deformed and 6 normal bighead carps with known deformities and normal body sizes bred in this laboratory. The specific process is as follows (the following is the amount used for each individual):

[0042]

[0043] After mixing...

Embodiment 2

[0063] Design and verification of primers based on long sequences related to deformity traits of gynogenetic bighead carp

[0064] (1) Design a pair of primers according to SEQ ID NO:2, the sequence of which is:

[0065] Forward primer F: TGTTGTCAATGCCCAATACC, as shown in SEQ ID NO:3;

[0066] Reverse primer R: CACAATGTGCCTACGCTGTT, as shown in SEQ ID NO:4;

[0067] (2) Select 65 gynogenetic bighead carps from the same batch of 6-month-old gynogenetic bighead carp cultured in our laboratory, including 34 deformed individuals and 31 normal individuals, and perform PCR verification on their genomic DNA samples. The PCR reaction procedure is as follows :

[0068] PCR loading information

[0069]

[0070]

[0071] PCR program settings

[0072]

[0073] (3) The amplified product is detected by electrophoresis on agarose, and the results are as follows: figure 1 As shown, a 475bp band can be amplified, the amplified product is sequenced by Sanger method, the sequencin...

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Abstract

The invention discloses a method for obtaining a molecular marker related to a malformation trait of gynogenesis hypophthalmichthys nobilis and application of the molecular marker. The molecular marker is obtained from a 2b-rad simplified genome of the gynogenesis hypophthalmichthys nobilis, and is located in the 3'UTR of a rgs7a gene. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:2, and an allelic mutation (G / A) exists a 531-position base of the sequence. The screened molecular marker is used to carry out association analysis on the malformation trait of the gynogenesis hypophthalmichthys nobilis, and can be applied in the auxiliary selection of the malformation trait of the gynogenesis hypophthalmichthys nobilis and body type breeding of the hypophthalmichthys nobilis.

Description

technical field [0001] The invention relates to the technical field of aquatic animal genetics and molecular marker-assisted selection breeding, in particular to a method for obtaining molecular markers related to deformity traits of gynogenetic bighead carp and its application. Background technique [0002] With the vigorous development of intensive aquaculture, fish deformities have received more and more attention. During the growth process of fish, bone deformity will lead to the decline of fish grabbing ability and feeding ability, which will affect growth and even die. The deformed fish that grow up cannot be sold and effectively processed because of their poor appearance, so the appearance of deformed fish will greatly affect the breeding benefits. Types of skeletal deformities include scoliosis, lordosis, operculum curvature, caudal vertebral deformities, and jaw deformities, etc. (Zheng Ke et al. Skeletal development and malformation in mariculture fish larvae and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 罗微微童金苟俞小牧王俊如周颖
Owner INST OF AQUATIC LIFE ACAD SINICA
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