Culture system for in-vitro culture of hedychium 'Woodlanders' and application of culture system

A technology of in vitro culture and culture system, which is applied in the field of plant tissue culture, can solve the problems of low transplant survival rate, high technical difficulty, and high cost, and achieve high transplant survival rate, improved survival rate, and simple method.

Active Publication Date: 2020-11-03
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the leaves of Zingiberaceae plants are longer and larger than other plant groups, and the water transpiration is very strong, there is a problem of low transplanting survival rate in the stage of transplanting sterile seedlings from the inside of the bottle to the outside of the bottle.
At this stage, very fine soil and air humidity management is often required to optimize the transplanting substrate, temperature, air humidity and light intensity, which is technically difficult and expensive

Method used

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  • Culture system for in-vitro culture of hedychium 'Woodlanders' and application of culture system
  • Culture system for in-vitro culture of hedychium 'Woodlanders' and application of culture system
  • Culture system for in-vitro culture of hedychium 'Woodlanders' and application of culture system

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Embodiment 1

[0044] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:

[0045] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize with 75% alcohol solution for 30 seconds on the ultra-clean workbench, then soak in 0.1% mercuric solution for 15 minutes. Rinse 6 times;

[0046]2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 2.0mg / L, the NAA of 0.1mg / L, the TDZ of 0.2mg / L, 25g sucrose and 8g / L agar;

[0047] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate the clustered buds for about 50 days; the clustered bud proliferation medium c...

Embodiment 2

[0054] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:

[0055] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize them with 75% alcohol solution for 60 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric solution for 10 minutes. Rinse 6 times;

[0056] 2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 4.0mg / L, the NAA of 0.05mg / L, the TDZ of 0.1mg / L, 30g sucrose and 7g / L agar;

[0057] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate for about 50 days to make the clustered buds proliferate; the clus...

Embodiment 3

[0063] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:

[0064] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize them with 75% alcohol solution for 45 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric solution for 12 minutes. Rinse 6 times;

[0065] 2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 3.0mg / L, the NAA of 0.7mg / L, the TDZ of 0.15mg / L, 28g sucrose and 7.5g / L agar;

[0066] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate for about 50 days to make the clustered buds proliferate; the cl...

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Abstract

The invention discloses a culture system for in-vitro culture of hedychium 'Woodlanders' and an application of the culture system, and belongs to the technical field of plant tissue culture. The culture system comprises a small rhizome induction culture medium, and the small rhizome induction culture medium is a sterile MS culture medium containing 1.0-3.0 mg / L of BA, 0.5-1.5 mg / L of NAA, 0.1-0.5mg / L of TDZ, 25-30 g of cane sugar and 7-8 g / L of agar. According to the culture system, base parts of cluster buds are induced to expand and proliferate into small rhizomes (aseptic seedlings are induced into the small rhizomes) in the previous stage of transplanting, so that nutrient and moisture storage capacity of the aseptic seedlings is improved, the adaptability of the aseptic seedlings isenhanced, and the survival rate of transplanting is increased. The aseptic seedlings obtained by the culture system have strong adaptability to seedling substrates and air humidity, the transplantingsurvival rate is 5-10% higher than that of conventional aseptic seedlings, and the survival rate after transplanting for 4 weeks can reach 96-100%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a culture system for in vitro culture of ginger flower, in particular to a culture system for in vitro culture of ginger flower and its application. Background technique [0002] Golden ginger flower (scientific name: Hedychium 'Woodlanders') is a perennial herbaceous plant of the Zingiberaceae genus Zingiberaceae. Because of its yellow-orange color, erect stalks and long flowering period, it has become the most popular variety in China besides the native traditional cultivar, Baijianghua. In addition, the flowers, roots, stems and fruits of Gingerflower have their special effects: certain ingredients can be extracted from the flowers and used in the blending of essences, which are fragrant and charming; Table, expelling wind and cold, warming meridians and relieving pain, expelling cold and other effects. [0003] Gingerflower seldom sets seeds and mainly reproduces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李永泉胡秀谭嘉川
Owner ZHONGKAI UNIV OF AGRI & ENG
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