Culture system for in-vitro culture of hedychium 'Woodlanders' and application of culture system
A technology of in vitro culture and culture system, which is applied in the field of plant tissue culture, can solve the problems of low transplant survival rate, high technical difficulty, and high cost, and achieve high transplant survival rate, improved survival rate, and simple method.
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Embodiment 1
[0044] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:
[0045] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize with 75% alcohol solution for 30 seconds on the ultra-clean workbench, then soak in 0.1% mercuric solution for 15 minutes. Rinse 6 times;
[0046]2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 2.0mg / L, the NAA of 0.1mg / L, the TDZ of 0.2mg / L, 25g sucrose and 8g / L agar;
[0047] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate the clustered buds for about 50 days; the clustered bud proliferation medium c...
Embodiment 2
[0054] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:
[0055] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize them with 75% alcohol solution for 60 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric solution for 10 minutes. Rinse 6 times;
[0056] 2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 4.0mg / L, the NAA of 0.05mg / L, the TDZ of 0.1mg / L, 30g sucrose and 7g / L agar;
[0057] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate for about 50 days to make the clustered buds proliferate; the clus...
Embodiment 3
[0063] The embodiment of a kind of golden ginger flower in vitro culture method of the present invention, comprises the steps:
[0064] 1) Select healthy and less polluted fresh rhizomes as explants. Under aseptic conditions, sterilize them with 75% alcohol solution for 45 seconds on the ultra-clean workbench, then soak them in 0.1% mercuric solution for 12 minutes. Rinse 6 times;
[0065] 2) cut off the leaf sheath wrapped on the tender stem with sterilized tweezers and blades, cut the tender stem into thin slices with one bud, inoculate it into the cluster bud induction medium, and cultivate for about 50 days to induce cluster buds; Bud induction medium is the sterile MS medium containing the BA of 3.0mg / L, the NAA of 0.7mg / L, the TDZ of 0.15mg / L, 28g sucrose and 7.5g / L agar;
[0066] 3) Cut the clustered buds obtained in step 2) into 1-2 units, transfer to the clustered bud proliferation medium, and cultivate for about 50 days to make the clustered buds proliferate; the cl...
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Abstract
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