Recycling of alkali sulfate rich waste water by biological pre-treatment with an extreme halophilic organism
A technology for alkali metal sulfates and halophilic microorganisms, which is applied to the treatment of wastewater containing alkali metal sulfates and the field of alkali metal sulfates, and can solve the problems of sodium ion migration hindrance and power consumption increase
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Embodiment 1
[0134] Example 1: Tolerance of Sodium Sulfate in Combination with Sodium Chloride
[0135] Halobacterium mediterranei (DSM 1411 ) is an extremely halophilic archaea that requires a minimum of 140 g / L NaCl for growth. Optimal growth was reported for a concentration of 170 g / L NaCl. The experiments described in this example should answer the following questions:
[0136] 1) Can HFX tolerate high concentrations of sodium sulfate?
[0137] 2) Can HFX degrade organic components when NaCl in the medium is partially replaced by Na2SO4
[0138] 3) Can HFX degrade components glycerol, phenol and formate in sulfate-rich wastewater
[0139] For part 1) of the experiment, HFX was cultured in a medium containing 150 g / L sodium chloride and sodium sulfate at a concentration of 0 to 100 g / L. In part 2), the sodium chloride in the medium was replaced with sodium sulfate. Because Na 2 SO 4 Contains two sodium molecules, so every mol of NaCl is replaced by 0.5 mol Na 2 SO 4 sub...
Embodiment 2
[0148] Embodiment 2: the change of the sodium sulfate concentration in the medium
[0149] 1) Can halobacteria mediterranean tolerate high concentrations of 50-400 g / L sodium sulfate without any NaCl
[0150] 2) Can HFX degrade organic components (e.g. glycerol, phenol) in sodium sulfate rich medium without any NaCl
[0151] For part 1) of the experiment, instead of sodium chloride, different concentrations of sodium sulfate were added to the synthetic HFX medium. Since the maximum solubility at 37°C is 480 g / L, sodium sulfate is added at a concentration of 50-400 g / L. Glycerol was used as a carbon source at a concentration of 2 g / L. Successful TOC reduction was shown when the metabolism of the cells reduced the glycerol concentration during the 5 days of culture.
[0152] When glycerol was fully degraded at 96 h, one shake flask per duplicate assay was pulsed with 0.1 g / L phenol and 2 g / L glycerol as the second substrate. The second shake flask was pulsed with 2 g / L...
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