Fermented feed rich in vitamin K2 and production method of fermented feed

A vitamin and feed technology, which is applied in the field of animal feed and bioengineering, can solve problems such as the difference in vitamin K2 content, and achieve the effect of rich content

Active Publication Date: 2020-11-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In solid-state fermentation, due to the difference in raw materials, strains and fermentation conditions, the content of vitamin K2 in the product will vary. In the feed industry, there is no

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] Bacillus amyloliquefaciens CCTCC NO:M 2019206 (disclosed in the patent application number CN 201910695004.9) was inoculated into LB medium, and cultivated at 37°C and 200r / min to a bacterial concentration of 1.0×10 9 CFU / mL.

[0029] Using 60% bran and 38% soybean meal as the main raw materials, adding dipotassium hydrogen phosphate (1.8g / 100g) and sodium glutamate (0.2g / 100g), according to the material-water ratio of 1:1.2 (w / w) After the water is mixed and stirred evenly, sterilize at 121℃ for 20 minutes, and insert 5% (5mL / 100g) of the Bacillus amyloliquefaciens CCTCC NO:M 2019206 culture solution according to the weight of the solid substrate and stir in the rotating shaft mechanically. Fermentation in the tank, stirring speed 10r / min, aeration rate of 0.1VVM, fermentation at 37±5℃, after 8 hours of fermentation, spraying and water replenishment, 1% of the material weight per hour of water replenishment; after 36 hours of fermentation, low-temperature ...

Example Embodiment

[0030] Example 2: No sodium glutamate added to the raw materials

[0031] With 60% bran and 38% soybean meal as the main raw materials, add dipotassium hydrogen phosphate (1.8g / 100g), mix with water at a material-to-water ratio of 1:1.2 (w / w) and stir evenly, at a temperature of 121 Sterilize under the condition of ℃ for 20 minutes, and insert 5% (5mL / 100g) of Bacillus amyloliquefaciens culture solution CCTCC NO:M 2019206 according to the weight of the solid substrate and ferment in a rotating shaft type mechanical stirring tank, stirring speed 10r / min, aeration The amount of 0.1VVM, fermentation at 37 ± 5 ℃, after 8 hours of fermentation, start spraying water supplement, the hourly water supplement is 1mL / 100mL material weight; after 36 hours of fermentation, low-temperature drying is performed to prepare feed. The final product vitamin K2 content is 88mg / kg.

Example Embodiment

[0032] Example 3: No dipotassium hydrogen phosphate added to the raw materials

[0033] With 61.8% bran and 38% soybean meal as the main raw materials, add sodium glutamate (0.2g / 100g), mix with water at a material-to-water ratio of 1:1.2 (w / w) and stir evenly, at a temperature of 121 Sterilize at ℃ for 20 minutes, insert 5% (5mL / 100g) of Bacillus amyloliquefaciens culture solution CCTCC NO:M 2019206 according to the weight of the solid substrate and ferment in a rotating shaft mechanical stirring tank, stirring at 10r / min, aeration Amount of 0.1VVM, fermentation at 37±5℃, after 8h of fermentation, start to spray and make up water, the hourly amount of water is 1% of the material weight; after 36h of fermentation, low-temperature drying is carried out. The final product vitamin K2 content is 72mg / kg.

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Abstract

The invention discloses a fermented feed rich in vitamin K2 and a production method of the fermented feed, and belongs to the technical field of animal feeds and bioengineering. The preparation methodcomprises the following steps of mixing bran and soybean meal which are used as main raw materials (98% or above) with dipotassium phosphate and sodium glutamate, and performing fermenting under theaction of microorganisms of bacillus amyloliquefaciens CCTCC NO: M 2019206 by adjusting the addition amount of water to obtain the feed. The content of the vitamin K2 in the fermented feed is increased by 200 times or above and reaches 100 mg/kg or above, and the number of spores in the fermented feed reaches 1.0 * 10 < 10 > CFU/g or above. The fermentation process is easy to control, the obtainedproduct is stable in quality, the prepared fermented feed is eaten by animals, the digestive absorption capacity of the animals can be improved, the microecological balance in the animals can be adjusted, and the disease prevention and resistance of the animals can be enhanced.

Description

technical field [0001] The invention relates to a fermented feed rich in vitamin K2 and a production method thereof, belonging to the technical fields of animal feed and bioengineering. Background technique [0002] Vitamin K2 is a fat-soluble vitamin mainly found in prokaryotes. During the growth of animals, vitamin K2 can increase the absorption and transport of calcium in the intestine, inhibit the excretion of calcium in urine and feces, reverse the loss of bone calcium, and significantly promote calcium balance. In addition, vitamin K2, as a coenzyme of glutamic acid γ-carboxylase, can also significantly increase the concentration of γ-carboxyglutamic acid (osteocalcin). Therefore, vitamin K2 is known as "uranium gold vitamin", which can prevent and treat osteoporosis and improve the growth performance of animals. [0003] Vitamin K2 is mainly synthesized by microorganisms. Although the intestinal tract of poultry can synthesize a small amount of vitamin K2, it is far...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/12A23K20/26A23K20/142
CPCA23K10/37A23K10/12A23K20/26A23K20/142Y02P60/87
Inventor 蔡国林陆健李存谢莹李晓敏吴殿辉
Owner JIANGNAN UNIV
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