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Fermented feed rich in vitamin K2 and production method of fermented feed

A vitamin and feed technology, which is applied in the field of animal feed and bioengineering, can solve problems such as the difference in vitamin K2 content, and achieve the effect of rich content

Active Publication Date: 2020-11-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In solid-state fermentation, due to the difference in raw materials, strains and fermentation conditions, the content of vitamin K2 in the product will vary. In the feed industry, there is no way to prepare fermented feed with high vitamin K2 through biotechnology treatment for use in feed formula. Balance the vitamin K2 content of raw materials to improve animal growth performance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Inoculate Bacillus amyloliquefaciens CCTCC NO:M 2019206 (disclosed in the patent application number CN 201910695004.9) into LB medium, and cultivate it at 37°C and 200r / min until the bacterial concentration is 1.0×10 9 CFU / mL.

[0029] With 60% of bran and 38% of soybean meal as the main raw materials, dipotassium hydrogen phosphate (1.8g / 100g) and sodium glutamate (0.2g / 100g) are added, and the ratio of material to water is 1:1.2 (w / w). After the water is mixed and stirred evenly, sterilize at 121°C for 20 minutes, add 5% (5mL / 100g) culture solution of Bacillus amyloliquefaciens CCTCC NO:M 2019206 according to the weight of the solid matrix in the rotating shaft mechanical stirring Fermentation in the tank, stirring speed 10r / min, air flow 0.1VVM, fermentation at 37±5°C, spray water replenishment after 8 hours of fermentation, the water replenishment amount per hour is 1% of the material weight; after 36 hours of fermentation, carry out low-temperature drying to obtain...

Embodiment 2

[0030] Embodiment 2: do not add sodium glutamate in the raw material

[0031] With 60% of bran and 38% of soybean meal as the main raw materials, dipotassium hydrogen phosphate (1.8g / 100g) is added, mixed with water at a material-to-water ratio of 1:1.2 (w / w) and stirred evenly, at a temperature of 121 Sterilize under the condition of ℃ for 20 minutes, add 5% (5mL / 100g) of Bacillus amyloliquefaciens CCTCC NO:M 2019206 culture solution according to the weight of the solid substrate, and ferment in the rotating shaft mechanical stirring tank, stirring speed 10r / min, ventilation Amount of 0.1VVM, fermented at 37±5°C, after 8 hours of fermentation, water replenishment was sprayed, and the water replenishment amount per hour was 1mL / 100mL of material weight; after 36 hours of fermentation, low-temperature drying was carried out to prepare the feed. The vitamin K2 content of the final product is 88mg / kg.

Embodiment 3

[0032] Embodiment 3: do not add dipotassium hydrogen phosphate in the raw material

[0033]With 61.8% of bran and 38% of soybean meal as the main raw materials, add sodium glutamate (0.2g / 100g), mix with water according to the material-water ratio of 1:1.2 (w / w) and stir evenly, at a temperature of 121 Sterilize under the condition of ℃ for 20 minutes, add 5% (5mL / 100g) of Bacillus amyloliquefaciens CCTCC NO:M 2019206 culture solution according to the weight of the solid substrate, and ferment in the rotating shaft mechanical stirring tank, stirring speed 10r / min, ventilation Amount of 0.1VVM, ferment at 37±5°C, start spraying water replenishment after 8 hours of fermentation, the water replenishment amount per hour is 1% of the material weight; carry out low-temperature drying after 36 hours of fermentation. The vitamin K2 content of the final product was 72mg / kg.

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Abstract

The invention discloses a fermented feed rich in vitamin K2 and a production method of the fermented feed, and belongs to the technical field of animal feeds and bioengineering. The preparation methodcomprises the following steps of mixing bran and soybean meal which are used as main raw materials (98% or above) with dipotassium phosphate and sodium glutamate, and performing fermenting under theaction of microorganisms of bacillus amyloliquefaciens CCTCC NO: M 2019206 by adjusting the addition amount of water to obtain the feed. The content of the vitamin K2 in the fermented feed is increased by 200 times or above and reaches 100 mg / kg or above, and the number of spores in the fermented feed reaches 1.0 * 10 < 10 > CFU / g or above. The fermentation process is easy to control, the obtainedproduct is stable in quality, the prepared fermented feed is eaten by animals, the digestive absorption capacity of the animals can be improved, the microecological balance in the animals can be adjusted, and the disease prevention and resistance of the animals can be enhanced.

Description

technical field [0001] The invention relates to a fermented feed rich in vitamin K2 and a production method thereof, belonging to the technical fields of animal feed and bioengineering. Background technique [0002] Vitamin K2 is a fat-soluble vitamin mainly found in prokaryotes. During the growth of animals, vitamin K2 can increase the absorption and transport of calcium in the intestine, inhibit the excretion of calcium in urine and feces, reverse the loss of bone calcium, and significantly promote calcium balance. In addition, vitamin K2, as a coenzyme of glutamic acid γ-carboxylase, can also significantly increase the concentration of γ-carboxyglutamic acid (osteocalcin). Therefore, vitamin K2 is known as "uranium gold vitamin", which can prevent and treat osteoporosis and improve the growth performance of animals. [0003] Vitamin K2 is mainly synthesized by microorganisms. Although the intestinal tract of poultry can synthesize a small amount of vitamin K2, it is far...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/12A23K20/26A23K20/142
CPCA23K10/37A23K10/12A23K20/26A23K20/142Y02P60/87
Inventor 蔡国林陆健李存谢莹李晓敏吴殿辉
Owner JIANGNAN UNIV
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