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A kind of macroporous hydrogel and its preparation method and application

A hydrogel and gelatin technology, which can be used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of difficult preparation of super-large pore hydrogels, unfavorable material biocompatibility, lack of uniform pore structure, etc., and achieve good Application prospect, excellent biocompatibility and degradation performance, good effect of biocompatibility

Active Publication Date: 2022-05-03
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the common methods for preparing biological macroporous hydrogels mainly include salt leaching method, phase separation method, freeze-drying method, gas foaming method, etc., but these methods often lack uniform and interconnected pore structure and pore size, making it difficult to prepare Hydrogels with ultra-large pore size, or the need to introduce additional chemicals during the preparation process to increase toxicity, which is not conducive to the biocompatibility of the material

Method used

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  • A kind of macroporous hydrogel and its preparation method and application
  • A kind of macroporous hydrogel and its preparation method and application
  • A kind of macroporous hydrogel and its preparation method and application

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Embodiment 1

[0049] Embodiment 1: a kind of hydrogel and preparation method thereof:

[0050] (1) Get 0.3g of gelatin microspheres with a particle size of about 230 μm and place them in a polytetrafluoroethylene mold, and gently tap the mold to make the gelatin microspheres tightly packed and smooth on the surface;

[0051] (2) Inject the ethanol solution into the stacked gelatin microspheres, and inject about 1 mL of ethanol solution with a volume concentration of 80% per gram of gelatin microspheres;

[0052] (3) Move the mold to a 70°C oven for 8 minutes;

[0053] (4) Take out the mold and freeze-dry to obtain the mutually bonded gelatin microsphere template, and its SEM image is as follows figure 2 As shown, the adjacent microspheres are slightly bonded at the interface, forming a mutually bonded gelatin microsphere template;

[0054] (5) Put the gelatin microsphere template of step (4) into 1 mL of 2% bis In the bonded hyaluronic acid solution, under the condition of -0.08MPa, the...

Embodiment 2

[0056] Embodiment 2: a kind of hydrogel and preparation method thereof:

[0057] (1) Sieve out gelatin microspheres with a particle size of about 100 μm, take 0.2 g of gelatin microspheres with a particle size of about 100 μm and place them in a polytetrafluoroethylene mold, tap the mold gently to make the gelatin microspheres tightly packed and the surface smooth ;

[0058] (2) Inject the ethanol solution into the stacked gelatin microspheres, and inject about 1 mL of ethanol solution with a volume concentration of 80% per gram of gelatin microspheres;

[0059] (3) Move the mold to an oven at 80°C for 5 minutes;

[0060] (4) Take out the mold and freeze-dry to obtain interconnected gelatin microsphere templates;

[0061] (5) Mix 1% double-bond hyaluronic acid solution and 10% double-bond gelatin solution at a volume ratio of 10:1, add 0.1% phenyl-2,4,6-trimethylbenzoylphosphinic acid Lithium, to obtain a mixed solution; the gelatin microsphere template of step (4) is put i...

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Abstract

The invention provides a macroporous hydrogel and its preparation method and application. In the preparation method, water-soluble gelatin microspheres are used as a porogen, and the interface of the gelatin microspheres is bonded to each other by heating to form a cross-linked gel. After removing the gelatin microsphere template, a macroporous hydrogel with controllable pore diameter and interconnectedness is finally formed. It is beneficial to cell adhesion, migration, proliferation, and transportation of oxygen and nutrients. It is expected to further develop pro-vascularization gel scaffolds and expand the application of hydrogels in tissue engineering and regenerative medicine. Compared with the macroporous hydrogel prepared by the traditional method, the macroporous hydrogel prepared by the present invention has a short gelation time and can be gelled in a few seconds. The pore structure is controllable, uniform and interconnected, and the pore size ranges from 30 μm to 600 μm. .

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a macroporous hydrogel and a preparation method and application thereof. Background technique [0002] Tissue engineering and regenerative medicine achieve tissue repair or regeneration by constructing tissue engineering scaffolds to simulate extracellular matrix and seed cells to form a complex. In order to better repair different damaged tissues, it is crucial to construct tissue engineering scaffolds with similar physical and chemical properties of extracellular matrix through biomimetic design. Hydrogel has been widely used in the field of tissue engineering and regenerative medicine because of its biomimetic structure highly similar to natural extracellular matrix, good mechanical properties, biocompatibility, and easy preparation. Three-dimensional culture and delivery system. [0003] However, the loaded cells are usually coated and confined in the submi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26A61L27/24A61L27/22A61L27/20A61L27/52A61L27/56A61L27/58C08L5/08C08L89/00C08L5/10C08L5/04
CPCC08J9/26A61L27/24A61L27/222A61L27/227A61L27/20A61L27/52A61L27/56A61L27/58C08J2207/10C08J2201/0464C08J2205/022C08J2305/08C08J2489/00C08J2389/00C08J2305/10C08J2305/04A61L2400/08C08L5/08
Inventor 鲁道欢于珊耿志杰裴大婷纪禹行国翠平刘群峰
Owner GUANGDONG PROV MEDICAL INSTR INST
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