Complex vitamin D for preventing and treating fungal infection and application of complex vitamin D

A multivitamin and fungal infection technology, applied in the field of medicine, can solve the problems of difficulty in fungal infection, fungal drug resistance, low price, etc., and achieve the effects of low cytotoxicity, thinning of bacterial fur, and reduced spore germination rate.

Active Publication Date: 2020-11-27
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional antifungal drugs are likely to make fungi resistant to drugs, which brings great difficulties to the clinical treatment of fungal infections.
In addition, traditional antifungal drugs have high liver and kidney toxic

Method used

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  • Complex vitamin D for preventing and treating fungal infection and application of complex vitamin D
  • Complex vitamin D for preventing and treating fungal infection and application of complex vitamin D
  • Complex vitamin D for preventing and treating fungal infection and application of complex vitamin D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 liquid-based diluent method drug susceptibility test (MIC 90 test)

[0032] Before the experiment, the three Aspergillus strains (A. fumigatus, Aspergillus flavus, and Aspergillus niger) were continuously replanted twice in YPD solid medium at 37°C, and Cryptococcus neoformans were cultured in YPD liquid medium at 37°C and 180rpm / min. 24h, to ensure the vigor and purity of the strains to ensure the vigor and purity of the strains. After the Aspergillus spores activated twice were eluted with sterile double distilled water containing 1% Tween 80, the Aspergillus spores and Cryptococcus neoformans were diluted with double distilled water, and then counted with a hemocytometer to determine the spore concentration. -1640 dilution to adjust the concentration of bacterial solution is 2 × 10 5 cfu / ml spare. Referring to the microdilution method of the CLSI M38-A protocol, add 100 μL of double-diluted multivitamin D to the 1st to 10th wells of the 96-well plate ...

Embodiment 2

[0036] Embodiment 2 in vitro antibacterial experiment

[0037] 1. Antibacterial experiment on Aspergillus

[0038] Prepare YPD solid medium containing different concentrations of complex vitamin D: mix vitamin D2 and vitamin D3 in different mass ratios, dissolve in DMSO to prepare a stock solution with a concentration of 50 mg / ml, and store at 4°C for later use.

[0039] Preparation of Aspergillus spore suspension: Before the experiment, the three Aspergillus strains were inoculated twice in YPD solid medium in a constant temperature incubator at 37°C to ensure the viability and purity of the strains. The twice-activated Aspergillus fumigatus spores were eluted with sterile double distilled water containing 1% Tween80, and Aspergillus spore suspensions of different concentrations were prepared with sterile double distilled water (10 5 cfu / ml, 10 4 cfu / ml, 10 3 cfu / ml) to carry out spot plate experiment, promptly take 3 μ l of spore suspension each time and spot on the prepa...

Embodiment 3

[0043] Example 3 Cytotoxicity Experiment

[0044] The cytotoxic effect of compound vitamin D (the mass ratio of vitamin D2 and 1,25-dihydroxyvitamin D3 is 1:2) on liver cancer cells (HepG2 cells) was detected by CCK-8 assay. Discard the culture medium for liver cancer cells with a growth confluence of about 90%, wash twice with PBS, add an appropriate amount of trypsin-EDTA, and store in 5% CO 2 , Incubate in a humid cell incubator at 37°C for 2 to 3 minutes. When the cells are plump and oval, add an appropriate amount of complete medium to stop the digestion, centrifuge, remove the supernatant, and collect the cells. Add an appropriate amount of regular cell complete medium, resuspend the cells, blow and mix gently with a pipette, count the cells, and use regular cell complete medium to adjust the cells to 5×10 4 / ml, and pipette evenly. The cell suspension was seeded into a 96-well plate at an amount of 100 μl / well, and 3 plates were planted (three replicates). Each 96-we...

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PUM

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Abstract

The invention discloses a complex vitamin D for preventing and treating fungal infection. The complex vitamin D comprises functional components of vitamin D2 and a vitamin D3 in the mass ratio of thevitamin D2 to the vitamin D3 being (1-3) to (1-5). The invention further discloses an application of the complex vitamin D to preparation of a medicine for preventing and treating fungal infection. The complex vitamin D has the effects of preventing and treating infection of aspergillus, cryptococcus neoformans and the like. The compound is low in cytotoxicity and can be used as a candidate for treatment of clinical anti-fungal infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a compound vitamin D capable of preventing and treating fungal infection and its application. Background technique [0002] Related studies have reported that vitamin D has a significant effect on the control of yeast fungi, but there are no reports on the control of Aspergillus fungi and Cryptococcus neoformans. Aspergillus, especially Aspergillus fumigatus, is an important opportunistic fungus capable of airborne transmission. In recent years, with the abuse of antibiotics, the treatment of immunosuppressants, the development of invasive equipment, the development of organ transplantation, etc., the infection rate of Aspergillus fumigatus has increased sharply, and the prognosis is poor. Two major fungal infectious diseases. What is more serious is the continuous emergence of drug-resistant Aspergillus fumigatus, which seriously threatens people's life and health. Cryptococ...

Claims

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Application Information

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IPC IPC(8): A61K31/593A61P31/10A61K31/592
CPCA61K31/592A61K31/593A61P31/10A61K2300/00
Inventor 宋章永张倩雷君文信彩岩毛樱逾王芬
Owner SOUTHWEST MEDICAL UNIVERISTY
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