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Serum carbon dioxide detection kit and preparation method thereof

A technology for detecting kits and carbon dioxide, which is applied in biochemical equipment and methods, measuring devices, determination/inspection of microorganisms, etc., can solve the problems of lower quality of reagents, poor stability of kits, etc., so as to increase the cost of reagents and simplify the preparation method. , the effect of improving stability

Pending Publication Date: 2020-11-27
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the NADH in the prior art carbon dioxide measurement kit degrades under the condition of pH7.5, causing the quality of the reagent to decline, and the stability of the kit is relatively poor

Method used

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  • Serum carbon dioxide detection kit and preparation method thereof
  • Serum carbon dioxide detection kit and preparation method thereof
  • Serum carbon dioxide detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Serum carbon dioxide detection kit and preparation method thereof

[0038] Reagent R:

[0039]

[0040]

[0041] The solvent is purified water, and finally, the pH value of the solution is adjusted within the range of 7.50±0.05 by using saturated Tris buffer and 1M HCl.

[0042] The preparation method of reagent R comprises the following steps: dissolving the Tris buffer salt in the above ratio in purified water, then adding the above ratio of EDTA disodium salt, magnesium acetate, sodium oxamate, and glucose, and stirring, Then add phosphoenolpyruvate and APADH, adjust the pH value of the solution to within the range of 7.50±0.05, finally add phosphoenolpyruvate carboxylase, malate dehydrogenase, glucose dehydrogenase, after mixing, the preparation Get reagent R, the reaction principle of kit in the present embodiment is as follows figure 2 shown.

Embodiment 2

[0044] Serum carbon dioxide detection kit and preparation method thereof

[0045] Reagent R:

[0046]

[0047]

[0048] The solvent is purified water, and finally, the pH value of the solution is adjusted within the range of 7.50±0.05 by using saturated Tris buffer and 1M HCl.

[0049] The preparation method of reagent R comprises the following steps: dissolving the Tris buffer salt in the above ratio in purified water, then adding the above ratio of EDTA disodium salt, magnesium acetate, sodium oxamate, and glucose, and stirring, Then add phosphoenolpyruvate and APADH, adjust the pH value of the solution to within the range of 7.50±0.05, finally add phosphoenolpyruvate carboxylase, malate dehydrogenase, glucose dehydrogenase, after mixing, the preparation Get reagent R, the reaction principle of kit in the present embodiment is as follows figure 2 shown.

Embodiment 3

[0051] Serum carbon dioxide detection kit and preparation method thereof

[0052]

[0053]

[0054] The solvent is purified water, and finally, the pH value of the solution is adjusted within the range of 7.50±0.05 by using saturated Tris buffer and 1M HCl.

[0055] The preparation method of reagent R comprises the following steps: dissolving the Tris buffer salt in the above ratio in purified water, then adding the above ratio of EDTA disodium salt, magnesium acetate, sodium oxamate, and glucose, and stirring, Then add phosphoenolpyruvate and APADH, adjust the pH value of the solution to within the range of 7.50±0.05, finally add phosphoenolpyruvate carboxylase, malate dehydrogenase, glucose dehydrogenase, after mixing, the preparation Get reagent R, the reaction principle of kit in the present embodiment is as follows figure 2 shown.

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Abstract

The invention discloses a serum carbon dioxide detection kit, and relates to the technical field of carbon dioxide detection. The kit comprises a reagent R: 50-150 mmol / L Tris buffer salt, 0.4-1.0 g / Lethylenediamine tetraacetic acid disodium salt, 1.0-3.0 g / L magnesium acetate, 1.0-3.0 g / L sodium oxamate, 10-20 g / L glucose, 0.3-1.7 g / L phosphoenolpyruvic acid, 0.2-2.2 g / L APADH, 1.2-3.8 KU / L malate dehydrogenase, 0.5-2.5 KU / L phosphoenolpyruvate carboxylase, and 1.0-2.0 KU / L glucose dehydrogenase. Purified water serves as a solvent. The invention also provides a preparation method of the kit.The method has the beneficial effects that: the problem of reagent quality reduction caused by APADH degradation under the condition that the pH value is 7.5 is solved, and meanwhile, the stability of the kit is improved.

Description

technical field [0001] The invention relates to the technical field of carbon dioxide detection, in particular to a serum carbon dioxide detection kit and a preparation method thereof. Background technique [0002] Carbon Dioxide and HCO in Serum 3 - In a state of balance, they together form a serum buffer system. The total concentration of carbon dioxide is the best indicator of serum electrolyte balance. Clinically, the total concentration of carbon dioxide is used to assist in the diagnosis of the acid-base balance of the respiratory system and metabolism. [0003] Serum CO 2 HCO 3 - The form exists, and its changes mainly reflect metabolic acid-base balance disorders. Serum CO 2 Increased, common in pyloric obstruction, Cushing's syndrome, taking too many alkaline drugs and so on. Respiratory acidosis: such as cor pulmonale, respiratory central depression, ventilator paralysis, emphysema, bronchiectasis, and pneumothorax. Serum CO 2 Reduced, common in severe dia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/527C12Q1/32
CPCC12Q1/527C12Q1/32G01N2333/904
Inventor 梁海港徐运李陈雨胡苗苗张金东吴泽东
Owner ANHUI IPROCOM BIOTECH CO LTD
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