Digestive tract submucosal injection short peptide hydrogel and application thereof

A hydrogel and digestive tract technology, which is applied to the submucosal injection of short peptide hydrogels in the digestive tract and the field of preparation thereof, can solve problems such as delayed gel formation, and achieves good biocompatibility, uniform and transparent appearance, and rapid temperature and the effect of ion response properties

Active Publication Date: 2020-12-01
CHINA JAPAN FRIENDSHIP HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liquid injection may also flow out to surrounding tissues and blood vessels, or cause unexpected results such as delayed gelation due to the influence of complex physiological environments such as body fluid dilution

Method used

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  • Digestive tract submucosal injection short peptide hydrogel and application thereof
  • Digestive tract submucosal injection short peptide hydrogel and application thereof
  • Digestive tract submucosal injection short peptide hydrogel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 14.23mg of Fmoc-YA powder and dissolve it in 1mL of PBS buffer (pH≈8), then vortex, sonicate and heat in a water bath at 70°C for 6 minutes to completely dissolve into a clear and transparent solution, and finally stand at room temperature for 10 minutes Serve as a gel, continue to stand overnight to get 30mM Fmoc-YA injectable hydrogel, the physical picture is as follows figure 1 As shown, the appearance is clear and transparent, and the texture is uniform.

Embodiment 2

[0031] Weigh 6.92mg of RGD and dissolve it in 1mL of PBS buffer, then weigh 20.66mg of Fmoc-YL powder and add it into 1mL of PBS buffer, vortex, sonicate and heat in a 75°C water bath for 5min to dissolve, then measure 0.5 mL of the above RGD solution and Fmoc-YL solution were mixed in equal proportions, and finally left at room temperature overnight to obtain a uniform and transparent injectable hydrogel co-assembled with Fmoc-YL (20 mM) and RGD (10 mM). Use the syringe filled with the above-mentioned hydrogel to inject regular geometric shapes on the surface of the glass slide, such as diagram 2-1 It is shown that the hydrogel has good injectability. In addition, in addition to the function of promoting cell adhesion, spreading and proliferation of RGD, the present invention unexpectedly found that RGD itself has multiple groups that can provide hydrogen bonding sites, and can be co-assembled with Fmoc-dipeptide molecules , thereby enhancing the hydrogen bonding inside the...

Embodiment 3

[0033] Weigh 5.68mg of Fmoc-RGD and dissolve it in 1mL of PBS buffer, then weigh 20.02mg of Fmoc-FL powder and add it into 1mL of PBS buffer, dissolve it by shaking, ultrasonication and heating in a water bath at 80°C for 4min, then measure Take 0.5 mL of the above two solutions and mix them in equal proportions, and finally stand at room temperature overnight to obtain a uniform and transparent injectable hydrogel co-assembled with Fmoc-FL (20 mM) and Fmoc-RGD (5 mM). Afterwards, samples were taken and their microscopic morphology was observed with an atomic force microscope, as shown in image 3 The nanofiber network shown.

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Abstract

The invention relates to a digestive tract submucosal injection short peptide hydrogel as well as a preparation method and application thereof. The hydrogel is formed by co-assembling amphiphilic dipeptide and RGD or Fmoc-RGD, the hydrogel has stimulation responsiveness, shear thinning and rapid self-repairing performance, is good in biocompatibility, safe, environmentally friendly, easy and convenient to operate, suitable for large-scale production, and good in upheaval effect and long in duration when applied to the aspect of ESD operation filler, so that the operation safety is improved, and the operation difficulty and time are reduced. In addition, the hydrogel can also be applied to the fields of biological nano materials, drug loading and controllable release, tissue repair and regeneration, cytoskeleton or tumor treatment and the like.

Description

technical field [0001] The invention relates to technical fields such as biomedical materials and injectable gels, and in particular to a short peptide hydrogel for submucosal injection of the digestive tract and its preparation method and application. The hydrogel has stimuli responsiveness, shear thinning and Fast self-healing performance. Background technique [0002] Endoscopic submucosal dissection (ESD), as an advanced treatment technique, is mainly used for the clinical diagnosis and treatment of early cancers and precancerous lesions of the digestive tract. Compared with traditional invasive surgery, it has a high one-time resection rate, low local recurrence rate, less trauma, and can effectively relieve patients' pain. However, due to the thin wall of the digestive tract, the difficulty of resection is high and the risk of perforation is likely to occur. Therefore, it is necessary to inject fillers into the submucosa (under the lesion) to assist the operation. At...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/18A61K31/704A61P35/00A61L27/22A61L27/52
CPCA61K9/0019A61K9/06A61K31/704A61K47/183A61L27/227A61L27/52A61L2400/06A61P35/00
Inventor 李靖涛白硕任朋赵洪川
Owner CHINA JAPAN FRIENDSHIP HOSPITAL
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