Rhodococcus pyridineophilus rp3 with deodorizing and growth-promoting functions and its application

A technology of Rhodococcus pyridineophilus and Rhodococcus pyridine, applied in the field of agricultural resources and environmental protection

Active Publication Date: 2022-04-08
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although previous studies have obtained strains that can degrade skatole, there is little research on how to maintain the activity of the degrading bacteria and its preparations. Therefore, research on the degrading bacteria and its fermentation process for composting odorous substances has been carried out, and the degrading bacteria with my country's independent intellectual property rights have been developed. It has important practical significance for the degradation of skatole, an odorous substance in livestock and poultry manure.

Method used

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  • Rhodococcus pyridineophilus rp3 with deodorizing and growth-promoting functions and its application
  • Rhodococcus pyridineophilus rp3 with deodorizing and growth-promoting functions and its application
  • Rhodococcus pyridineophilus rp3 with deodorizing and growth-promoting functions and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Isolation and Identification of Rhodococcus pyridinovorans Rp3

[0042] (1) Isolation of skatole-degrading bacteria Rp3 strain

[0043] The Rhodococcus pyridinovorans Rp3 of the present invention is obtained from sheep manure composted soil, after enrichment, with skatole as the only nutrient, and after dilution and coating, it is separated and obtained, specifically as follows:

[0044] (1) Isolation of skatole-degrading bacteria

[0045] Samples were taken from different sheep manure piles in Yingxian County, Shanxi Province, and samples were taken after multiple sampling points, each sample 500g. cryopreserved and brought back to the laboratory. Add the mother liquor of skatole into a 250mL Erlenmeyer flask, add 60mL of inorganic salt culture solution L2 after the ethanol volatilizes, shake and mix well, so that the final concentration of skatole is 10mg·L -1 . Add 0.1g of composted soil samples into the culture medium, take the culture medium without ...

Embodiment 2

[0059] Embodiment 2, the growth characteristic of Rhodococcus Rp3 bacterial strain

[0060] (1) Effect of temperature on the growth of Rhodococcus Rp3

[0061] Pick a few colonies and dilute to 10 -4 、10 -5 , coated on LB solid plates, placed in 28°C, 32°C, 35°C, 37°C incubators respectively, with 3 repetitions for each temperature gradient, and observed and measured the colony diameter at the 2nd and 7th maximum.

[0062] The results showed that: 28~32℃ was more suitable for the growth of the strain (Table 2).

[0063] Table 2 Effects of different temperatures on the colony expansion speed of Rhodococcus Rp3

[0064]

[0065] Note: Different letters in the table indicate significant difference (p≤0.05)

[0066] (2) Effect of NaCl content on the growth of Rhodococcus Rp3

[0067] The strains were inoculated in LB culture solution with NaCl contents of 0%, 2%, 4%, 6%, 8%, 10%, 12%, and 14%, respectively, at 28°C, 180r·min-1 Shake culture for 48h, measure OD 600 value. ...

Embodiment 3

[0078] Embodiment 3, Rhodococcus Rp3 bacterial strain can remove the inhibitory effect of skatole on the growth of cucumber seedlings

[0079] Cucumber seed germination treatment: bake cucumber seeds at 54°C for 3 hours, let them cool, then sterilize them with 1% NaClO for 1 minute, wash them with sterile water for 5 times, then transfer them to moist sterile filter paper, put them at 25°C to let the cucumbers The seeds germinate.

[0080] Pour L2 medium into a 9 cm two-part Petri dish. Accurately weigh 0.99g of silica and add 0.01g of skatole after high-temperature sterilization, mix evenly, weigh 25mg of the mixture and add it to one side of the plate, so that the content of skatole is 250μg, and put 3 Lubai on the other side of the plate Cucumber seeds of cucumber, CK is adding the same amount of silicon dioxide, treatment T1 is adding silicon dioxide, skatole mixture and the concentration is 2×10 7 Rp3 bacteria suspension, T2 is equal amount of silica and skatole mixture...

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Abstract

The invention relates to a strain of rhodococcus pyridineophilus which degrades the odorous substance skatole in compost and promotes plant growth and application thereof. The preservation number of the Rhodococcus pyridinovorans Rp3 provided by the present invention in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No. 20444. The strain was isolated from the soil under sheep manure compost. It has a high degradation rate for high-concentration compost odorous substance-skatole, and can relieve the inhibitory effect of high-concentration skatole on plant growth. The strain has simple nutritional requirements, low production cost, and high salt tolerance, which is beneficial for its application in the treatment and purification of livestock and poultry manure pollution environment, and has advantages over other degrading bacteria agents, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of agricultural resources and environmental protection, and in particular relates to a strain of rhodococcus pyridineophilus which can efficiently degrade compost odorous substance skatole and an application thereof. [0002] technical background [0003] Nitrogen heterocyclic compounds are a kind of refractory organic compounds with wide pollution and high toxicity. Most nitrogen heterocyclic compounds have foul or pungent odors, among which indole nitrogen heterocyclic organic compounds are the main components of organic pollutants in livestock compost, which cause the deterioration of the farm and its surrounding environment, causing a series of environmental pollution and public health problems. 3-Methylindole, commonly known as skatole, is a derivative of indole and one of the main metabolites of tryptophan. Although 3-methylindole can regulate the intestinal flora and inhibit the proliferation of vario...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01
CPCC12N1/20C02F11/02C12R2001/01C12N1/205
Inventor 郭荣君李世东吴玉洪张世昌
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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