Intestinal flora related to immune recovery and application thereof

A technology of intestinal flora and intestinal tract, which is applied in the field of microbiology and medical molecular biology, can solve the problems that the recovery process of immunocompromised patients is not clear and affects HIV patients, so as to improve the level of immune recovery, and the detection is convenient and fast , The effect of convenient sample collection

Inactive Publication Date: 2020-12-01
翊康生物科技发展(辽宁)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, which components of the gut microbiota and how they affect the recov

Method used

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  • Intestinal flora related to immune recovery and application thereof
  • Intestinal flora related to immune recovery and application thereof
  • Intestinal flora related to immune recovery and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0073] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular Cloning Experiment Manual (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions. Example 1 After ART treatment, the intestinal flora of patients with immune recovery is enriched with more lactic acid bacteria compared with patients with poor recovery

[0074] After 2 years of ART treatment, patients with immune recovery (IR) and patients with poor immune recovery (INR) were studied. Among them, IR and INR mean that after 2 years of ART treatment, the HIV virus is completely suppressed, and the CD4+ T cell count is greater than 350 / mm 3 and less than 350 / mm 3 of HIV patients.

[0075] Metagenomic da...

Embodiment 2

[0081] Example 2 The lactic acid production and metabolism of patients with immune recovery is significantly higher than that of patients with poor recovery

[0082] Using metagenomic sequencing data to quantitatively evaluate the abundance of key enzymes in the lactic acid production pathway of each patient's intestinal flora, the potential of lactic acid production in the intestinal flora of IR and INR patients in Example 1 was analyzed. Lactate dehydrogenase (LDH) is the key enzyme in the conversion of pyruvate to lactate during anaerobic glycolysis, and all lactate is produced from LDH. LDH has two forms L-LDH and D-LDH which produce L-lactic acid and D-lactic acid respectively. By annotating the abundance of each metabolic pathway and enzyme in the metagenomic data, such as Figure 2a As shown, the abundance (EC1.1.1.28) of D-LDH in the IR group was significantly higher than that in the INR group, with the abundance value >3.5×10 -6 As the threshold, IR and INR patients...

Embodiment 3

[0083] Example 3 Compared with patients with poor immune recovery, lactic acid bacteria functional groups are more enriched in the intestinal tract of patients with immune recovery

[0084] Interacting species in the microbiota are often organized into functional groups and perform metabolic functions as a whole. Using metagenomic sequencing data, the intestinal flora of IR and INR patients in Example 1 was analyzed. Such as Figure 3a As shown in , by evaluating the abundance associations between all species that appeared, a total of 9 species groups that were strictly correlated in abundance (rho>0.8) were found. Of these, the two largest species groups (clusters I and II) both contained large numbers of lactic acid bacteria: cluster I had 13 species, of which 9 were lactic acid bacteria, and cluster II had 5 species, of which 4 were lactic acid bacteria. These lactic acid bacteria are mainly from the genera Streptococcus, Gemella and Granulicatella, and were identified as...

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Abstract

The invention provides an intestinal flora related to immune recovery and application thereof. As found in the invention for the first time, specific intestinal bacteria species have the effect of promoting body immune recovery. A biomarker related to immune recovery provided by the invention comprises 32 intestinal bacteria species, dextral lactate dehydrogenase and 18 lactic acid bacteria species. By utilizing the biomarker, risk assessment can be carried out on immune recovery prognosis after immune impairment of a patient, accuracy, sensitivity and specificity are high, sample collection is convenient, no body injury is generated, detection is convenient and fast, regular detection can be carried out, and the intestinal species can be used to develop preventive and therapeutic productsand assist the treatment of patients with impaired immune functions in order to reduce the incidence rate of poor immune recovery.

Description

technical field [0001] The present invention relates to the technical fields of microbiome and medical molecular biology, in particular, relates to intestinal flora related to immune recovery and its application. Background technique [0002] CD4+ cells (CD4+T cells) are damaged, and the count decrease is the most important pathological change after HIV infection and the main reason leading to acquired immunodeficiency syndrome (AIDS). Therefore, the primary goal of treating HIV infection is to restore the patient's CD4+ cell count. Antiretroviral therapy (ART) can effectively suppress viral replication in HIV-infected patients, and normalize CD4+ cell counts (>500 / mm 3 ), known as immune recoverers (immune responders, immune responders, IRs). However, even after years of antiretroviral therapy, 15-20% of patients still have CD4+ cell counts below 500 / mm3, and these patients are called poor immune recovery (immune non-responders, immune non-responders, INRs ). In INRs...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/689C12N15/11A61K35/744A61P37/04A61P1/00
CPCC12Q1/6883C12Q1/689A61K35/744A61P37/04A61P1/00C12Q2600/118Y02A50/30
Inventor 宜丹周诗康康禹
Owner 翊康生物科技发展(辽宁)有限公司
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