PH-responsive adriamycin carrier-free nano-drug as well as preparation method and application thereof

A nano-drug and doxorubicin technology, applied in the field of biomedical nano-materials, can solve the problems of low drug loading and high toxicity of organic solvents, and achieve the effects of clear components, easy quality control, and green and simple preparation process.

Pending Publication Date: 2020-12-04
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, the carboxyl-rich citrate and the amine group on doxorubicin are hydrogen-bonded to form n

Method used

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  • PH-responsive adriamycin carrier-free nano-drug as well as preparation method and application thereof
  • PH-responsive adriamycin carrier-free nano-drug as well as preparation method and application thereof
  • PH-responsive adriamycin carrier-free nano-drug as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] Preparation of pH-responsive doxorubicin carrier-free nanomedicines

[0032] (1) Take a brown reaction bottle, first add 1.0 mL of 1.0 M sodium citrate solution, then add 200 μg DOX to it, and use an ultrasonic instrument to assist to fully dissolve and mix the material;

[0033] (2) place the above-mentioned mixed solution on an infrared heating electromagnetic stirrer to react in the dark at room temperature for 6h;

[0034] (3) High-speed centrifugation at 12,000 rpm after the reaction is completed, and washing with sodium citrate solution 4 times, after washing is completed, freeze-drying can obtain the target product. All washing solutions were collected in a brown reagent bottle and stored in the dark until the drug loading of doxorubicin was determined. The absorbance of doxorubicin at 486nm was detected by UV-Vis spectrometry, and the adsorption amount of DOX was calculated. The calculation showed that the loading rate of doxorubicin could b...

Example Embodiment

[0035] Example 2

[0036] Preparation of pH-responsive doxorubicin carrier-free nanomedicines

[0037] (1) Take a brown reaction bottle, first add 1.0 mL of 1.0 M sodium citrate solution, then add 600 μg DOX to it, and use an ultrasonic instrument to assist to fully dissolve and mix the material uniformly;

[0038] (2) place the above-mentioned mixed solution on an infrared heating electromagnetic stirrer to react in the dark at room temperature for 6h;

[0039] (3) High-speed centrifugation at 12,000 rpm after the reaction is completed, and washing with sodium citrate solution 4 times, after washing is completed, freeze-drying can obtain the target product. All washing solutions were collected in a brown reagent bottle and stored in the dark until the drug loading of doxorubicin was determined. The absorbance of doxorubicin at 486nm was detected by UV-Vis spectrometry, and the adsorption amount of DOX was calculated. The calculation showed that the loading rate of doxorubic...

Example Embodiment

[0040] Example 3

[0041] Preparation of pH-responsive doxorubicin carrier-free nanomedicines

[0042] (1) Take a brown reaction bottle, first add 1.0 mL of 1.0 M sodium citrate solution, then add 1000 μg DOX to it, and use an ultrasonic instrument to assist to fully dissolve and mix the material uniformly;

[0043] (2) place the above-mentioned mixed solution on an infrared heating electromagnetic stirrer to react in the dark at room temperature for 6h;

[0044] (3) High-speed centrifugation at 12,000 rpm after the reaction is completed, and washing with sodium citrate solution 4 times, after washing is completed, freeze-drying can obtain the target product. All washing solutions were collected in a brown reagent bottle and stored in the dark until the drug loading of doxorubicin was determined. The absorbance of doxorubicin at 486nm was detected by UV-Vis spectrometry, and the adsorption amount of DOX was calculated. The calculation showed that the loading rate of doxorubi...

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Abstract

The invention provides a pH-responsive doxorubicin carrier-free nano-drug as well as a preparation method and application thereof The preparation method of the pH-responsive doxorubicin carrier-free nano-drug is characterized in that citrate rich in carboxyl is combined with amido on doxorubicin through hydrogen bonds to prepare the pH-responsive doxorubicin carrier-free nano-drug. The pH-responsive doxorubicin carrier-free nano-drug avoids toxic and side effects caused by introduction of a nano-carrier, has the advantages of high safety, strong stability, strong drug release capability and the like, is mild in reaction conditions, green and simple in synthesis process, cheap and easily available in raw materials and easy for industrial large-scale production, and can be applied to preparation of antitumor drugs.

Description

technical field [0001] The invention relates to the technical field of biomedical nanomaterials, in particular to a pH-responsive doxorubicin carrier-free nanomedicine, a preparation method thereof, and an application of the medicine in the preparation of antitumor medicines. Background technique [0002] At present, the main treatment methods for cancer include surgery, chemotherapy, and radiotherapy, as well as molecular targeted therapy that has emerged in recent years. But in fact, these treatment methods all have certain disadvantages. They cannot eradicate all cancer cells, and will cause cancer recurrence, and at the same time, some normal cells will be damaged, causing toxic and side effects to the body. [0003] At present, the nanotechnology drug delivery system is a hot spot in medical research. It loads anti-tumor drugs with hydrogen bond force, accurately delivers the drugs to the lesion, reduces the toxicity of free doxorubicin, and realizes accurate diagnosis ...

Claims

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Application Information

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IPC IPC(8): A61K47/54A61K47/69A61K31/704A61P35/00
CPCA61K47/542A61K47/6929A61K31/704A61P35/00
Inventor 李林张彩凤宋吉祥
Owner TAIYUAN NORMAL UNIV
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